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Cellulases : methods and protocols

Author: Mette Lübeck
Publisher: New York, NY : Humana Press : Springer, [2018] ©2018
Series: Methods in molecular biology (Clifton, N.J.), v. 1796
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This volume discusses the latest research and techniques used to study novel cellulases. The chapters in this book explore topics such as the role of cellulases in lignocellulose deconstruction for generation of a sugar platform for biofuel; screening for novel fungal b-glucosidases in a variety of fungal strains; discovery of novel enzymes in bacteria or fungi; protocols for isolating cellulolytic rumen bacteria;  Read more...
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Genre/Form: Electronic books
Laboratory Manual
Laboratory manuals
Additional Physical Format: Print version:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Mette Lübeck
ISBN: 9781493978779 1493978772 9781493978762 1493978764
OCLC Number: 1038534937
Description: 1 online resource (xiii, 324 pages) : illustrations (some color).
Contents: Preface...Table of Contents...Contributing Authors...Part I Introduction Chapter and a Case Example1. Cellulases: Role in Lignocellulosic Biomass UtilizationSanjeev Kumar Soni, Amita Sharma, and Raman Soni2. Discovery of a Novel Fungus with an Extraordinary b-GlucosidasePeter Stephensen Lubeck and Mette LubeckPart II Protocols Discovery of New Cellulases Using Classical or Molecular Methods3. Isolation and Screening of Cellulolytic Filamentous FungiMette Lubeck and Peter Stephensen Lubeck4. Isolation and Screening of Cellulose Degrading Microorganisms from Different Ecological NichesAyyappa Kumar Sista Kameshwar and Wensheng Qin5. Isolation of Cellulolytic Bacteria from the RumenMakoto Mitsumori6. Methods for Discovery of Novel Cellulosomal Cellulases using Genomics and Biochemical ToolsYonit Ben-David, Bareket Dassa, Lizi Bensoussan, Edward A. Bayer, and Sarah Morais7. Metatranscriptomic Techniques for Identifying Cellulases in Termites and Their SymbiontsBrittany F. Peterson and Michael E. Scharf8. Discovery of Novel Cellulases Using Proteomic StrategiesMarta Zoglowek, Heather Brewer, and Angela Norbeck9. Identification of Key Components for the Optimization of Cellulase Mixtures Using a Proteomic StrategyJingyao Qu, Jing Zhu, Guodong Liu, and Yinbo Qu10. Cloning and Expression of Heterologous Cellulases and Enzymes in Aspergillus nigerMorgann C. Reilly, Saori Amaike Campen, Scott E. Baker, John M. Gladden, and Jon K. Magnuson11. Advanced Cloning Tools for Construction of Designer CellulosomesAmaranta Kahn, Edward A. Bayer, and Sarah Morais12. Evaluation of Thermal Stability of Cellulosomal Hydrolases and Their complex FormationAmaranta Kahn, Anastasia P. Galanopoulou, Dimitris G. Hatzinikolaou, Sarah Morais, and Edward A. BayerAssays for Activity Testing13. Endoglucanase (EG) Activity AssaysM. Shafiqur Rahman, Sheran Fernando, Brian Ross, Jiangning Wu, and Wenshing Qin14. Cellobiohydolase (CBH) Activity AssaysHem Kanta Sharma, Wensheng Qin, and Chunbao (Charles) Xu15. Two-Dimensional High-Throughput Endo-Enzyme Screening Assays Based on Chromogenic Polysaccharide Hydrogel and Complex Biomass SubstratesJulia Schuckel and Stjepan Kresimir Kracun16. Analytical Tools for Characterizing Cellulose-Active Lytic Polysaccharide Monooxygenases (LPMOs)Bjorge Westereng, Jennifer S.M. Loose, Gustav Vaaje-Kolstad, Finn L. Aachmann, Morten Sorlie, and Vincent G.H. Eijsink17. Light Induced Electron Transfer Protocol for Enzymatic Oxidation of PolysaccharideDavid Cannella18. Purification of Cellulase from Cellulolytic Microorganisms and Characterization of the Total Cellulase Activities (TCA)Ayyappa Kumar Sista Kameshwar and Wensheng QinApplication of Cellulases for Lignocellulose Degradation19. Fungal On-Site Production of Cellulolytic Enzymes by the Sequential Cultivation MethodCristiane S. Farinas, Camila Florencio, and Alberto C. Badino20. Test of Efficacy of Cellulases for Biomass DegradationHenning JoergensenBioinformatics for Improving Cellulases21. Homology Modeling for Enzyme DesignWimal Ubhayasekera
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1796
Responsibility: edited by Mette Lübeck.

Abstract:

This volume discusses the latest research and techniques used to study novel cellulases. The chapters in this book explore topics such as the role of cellulases in lignocellulose deconstruction for generation of a sugar platform for biofuel; screening for novel fungal b-glucosidases in a variety of fungal strains; discovery of novel enzymes in bacteria or fungi; protocols for isolating cellulolytic rumen bacteria; analysis of enzyme mixtures produced by ligocellulolytic fungi; and homology modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Cellulases: Methods and Protocols is a valuable resource for biochemists, molecular biologists, chemical engineers, and industrial researchers interested in this field.

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