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Chaperones : methods and protocols

Author: Stuart K Calderwood; Thomas L Prince
Publisher: New York, NY : Humana Press, [2018]
Series: Methods in molecular biology (Clifton, N.J.), v. 1709.
Edition/Format:   eBook : Document : EnglishView all editions and formats
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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Chaperones.
New York, NY : Humana Press, [2018]
(OCoLC)1000603649
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Stuart K Calderwood; Thomas L Prince
ISBN: 9781493974771 1493974777
OCLC Number: 1013877649
Description: 1 online resource (xix, 445 pages) : illustrations (some color)
Contents: 1. Targeted Deletion of Hsf1, 2 and 4 Genes in Mice Xiongjie Jin, Binnur Eroglu, Demetrius Moskophidis, and Nahid F. Mivechi 2. Role of Heat Shock Factors in Stress-induced Transcription Murshid, A., Prince T., Ben Lang, and Calderwood, S. K. 3. Monitoring of the Heat Shock Response with a Real-time Luciferase Reporter Toshiki Kijima, Takanori Eguchi, Len Neckers, and Thomas L. Prince 4. Quantitative Profiling of Chaperone/client Interactions with LUMIER Assay Mikko Taipale 5. Measurement of chaperone-mediated effects on Polyglutamine Protein Aggregation by the Filter Trap Assay Maria A.W.H. van Waarde-Verhagen and Harm H. Kampinga 6. Fluorescent-linked Enzyme Chemoproteomic Strategy (FLECS) for Identifying HSP70 Inhibitors Haystead, T.A.J. 7. A High-throughput Screen for Inhibitors of the Hsp90-Chaperone Machine Jason Davenport, Lakshmi Galam, and Robert L. Matts 8. Primary Colorectal Cells Culture as a Translation Research Model Sheah Lin Lee, Nina Claire Dempsey-Hibbert, Dale Vimalachandran, Terence David Wardle, Paul A Sutton, and John H. H. Williams 9. Cell Death and Survival Assays Alexander E. Kabakov and Vladimir L. Gabai 10. Detecting the Potential Pharmacological Synergy of Drug Combination by Viability Assays In Vitro Benjamin K. Gibbs and Carole Sourbier 11. Proteomic Profiling of Hsp90 Inhibitors Sudhakar Voruganti, Jake T. Kline, Maurie J. Balch, Janet Rogers, Robert L. Matts, and Steven D. Hartson 12. Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and its Interaction with Specific Client Polypeptides Andre-Patrick Arrigo 13. Nucleotide Exchange Factors for Hsp70 Chaperones Heike Rampelt, Matthias P. Mayer, and Bernd Bukau 14. Determination of Hsp90 Activity through Activation of Glucocorticoid Receptors in Yeast Michael Reidy 15. Bacterial Hsp90 ATPase Assays Joel R. Hoskins, Sue Wickner, and Shannon M. Doyle 16. Detecting Post-translational Modifications of Hsp90 Rebecca A. Sager, Mark R. Woodford, Len Neckers, and Mehdi Mollapour 17. Chromatin Immunoprecipitation (ChIP) of Heat Shock Protein 90 (Hsp90) Aneliya Yoveva and Ritwick Sawarkar 18. A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond Benjamin J. Lang, Kristina M. Holton, Jianlin Gong, and Stuart K. Calderwood 19. Computational Modeling of the Hsp90 Interactions with Cochaperones and Small Molecule Inhibitors Gennady M. Verkhivker 20. Computational Analysis of the Chaperone Interaction Networks Shwani Kumar, Kamran Rizzolo, Sandra Zilles, Mohan Babu, and Walid A. Houry 21. Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer Francesco Cappello, Everly Conway de Macario, Francesca Rappa, Giovanni Zummo, and Alberto J. L. Macario 22. Immunohistochemical and Flow Cytometric Analysis of Intracellular and Membrane-bound Hsp70, as a Putative Biomarker of Glioblastoma Multiforme, using the cmHsp70.1 Monoclonal Antibody Stefan Stangl, Gemma A. Foulds, Helena Fellinger, Geoffrey J. Pilkington, A. Graham Pockley, and Gabriele Multhoff 23. Detection and Analysis of Extracellular Hsp90 (eHsp90) Stephanie Cortes, Alexander Baker-Williams, Mehdi Mollapour, and Dimitra Bourboulia 24. Molecular Chaperone Receptors Ayesha Murshid, Jimmy Theriault, Jianlin Gong, and Stuart K. Calderwood 25. Creation of Recombinant Chaperone Vaccine using Large Heat Shock Protein for Antigen-targeted Cancer Immunotherapy Chunqing Guo, John R. Subjeck, and Xiang-Yang Wang 26. A Novel Heat Shock Protein 70-based Vaccine Prepared from DC-Tumor Fusion Cells Desheng Weng, Stuart K. Calderwood, and Jianlin Gong 27. Hsp70: A Cancer Target Inside and Outside the Cell Christophe Boudesco, Sebastien Cause, Gaetan Jego, and Carmen Garrido 28. Evidence for Hsp90 Cochaperones in Regulating Hsp90 Function and Promoting Client Protein Folding Marc B. Cox and Jill L. Johnson 29. Clinical Evaluation and Biomarker Profiling of Hsp90 Inhibitors Akira Yuno, Min-Jung Lee, Sunmin Lee, Yusuke Tomita, David Rekhtman, Brittni Moore, and Jane B. Trepel
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1709.
Responsibility: edited by Stuart K. Calderwood, Thomas L. Prince.

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