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Chemistry of protein conjugation and cross-linking

Author: Shan S Wong
Publisher: Boca Raton : CRC Press, ©1993.
Edition/Format:   Print book : EnglishView all editions and formats

Chemical cross-linking reagents have attained great practical use in industry as well as in basic research. This book offers an explanation of the underlying mechanism of chemical modification,  Read more...


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Genre/Form: Aufsatzsammlung
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Shan S Wong
ISBN: 0849358868 9780849358869
OCLC Number: 48130492
Description: 340 pages : illustrations ; 27 cm
Contents: INTRODUCTIONREACTIVE GROUPS OF PROTEINS AND THEIR MODIFYING AGENTSCompositions of ProteinsFunctional Groups of ProteinsGroup Directed ReagentsCHOICE AND DESIGN OF CROSS-LINKING REAGENTSNucleophilic ReactionsElectrophilic ReactionsGroup Directed ReactionsNonspecific ReactionsCleavable ReagentsLabeled Cross-Linking ReagentsHydrophobicity and Hydrophilicity of ReagentsHOMOBIFUNCTIONAL CROSS-LINKING REAGENTSAmino Group Directed Cross-LinkersSulfhydryl Group Directed Cross-LinkersCarboxyl Group Directed Cross-Linking ReagentsPhenolate and Imidazolyl Group Directed Cross-Linking ReagentsArginine Residue Directed Cross-LinkerNondiscriminatory Photoactivatable Cross-LinkersNoncovalent Homobifunctional Cross-Linking ReagentsMiscellaneous Bifunctional ReagentsHETEROBIFUNCTIONAL CROSS-LINKERSGroup Selective Heterobifunctional Cross-Linking ReagentsPhotosensitive Heterobifunctional Cross-Linking ReagentsDisguised or Masked ReagentsZERO-LENGTH CROSS-LINKING REAGENTSCarboxyl Group Activating ReagentsReagents for Carbohydrate ActivationReagents for Disulfide FormationEnzymes as Zero-Length Cross-LinkersPROCEDURES, ANALYSIS, AND COMPLICATIONSProcedures for Cross-LinkingAnalysis of Cross-Linked ProductsReaction ComplicationsAPPLICATION OF CHEMICAL CROSS-LINKING TO SOLUBLE PROTEINSInterresidue Distance DeterminationsNearest Neighbor AnalysisConformational AnalysisStructural StabilityCROSS-LINKING OF MEMBRANE PROTEINSGeneral Methodology of Membrane Cross-LinkingPossible Artifacts of Membrane Cross-LinkingIdentification of Membrane ReceptorsNearest-Neighbor Analysis of Membrane ComponentsMembrane Protein Conformational AnalysisStabilization of Membrane ProteinsPREPARATION OF ENZYME IMMUNOCONJUGATES AND OTHER IMMUNOASSAY COMPONENTSComponents of Enzyme ImmunoassaysConjugation of Enzymes to Antibodies and their FragmentsCoupling Proteins to Antibodies and their FragmentsCoupling Enzymes to Proteins and AntigensCharacterization of Conjugation MethodsPREPARATION OF IMMUNOTOXINS AND OTHER DRUG CONJUGATES FOR TARGETING THERAPEUTICSSelection of Cross-Linking Reagents and ToxinsConjugation through Disulfide BondConjugation through Thioether LinkageConjugation with Actuvated ChlorambucilConjugation with Acid-Labile and Photocleaveable Cross-LinkersConjugation through Carbohydrate ResiduesPreparation of Immunotoxins Using Avidin-Biotin LinkageMiscellaneous Coupling MethodsCONJUGATION OF PROTEINS TO SOLID MATRICESFunctionalities of MatricesProtein Immobilization by Matrix ActivationCross-Linking Reagents Used in Protein ImmobilizationCross-Linking through Carbohydrate ChainsOTHER APPLICATIONS AND FUTURE PROSPECTSApplication to Nucleic AcidsCross-Linking Small Molecules to ProteinsSynopsisINDEXNote: Each chapter also contains an Introduction and References
Responsibility: Shan S. Wong.


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