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Chromatin and chromosomal protein research : II

Author: Gary Stein; Janet L Stein; Lewis J Kleinsmith
Publisher: New York : Academic Press, 1978.
Series: Methods in cell biology, v. 17.
Edition/Format:   Print book : EnglishView all editions and formats
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Document Type: Book
All Authors / Contributors: Gary Stein; Janet L Stein; Lewis J Kleinsmith
ISBN: 0125641176 9780125641173
OCLC Number: 15116597
Description: xix, 434 pages : illustrations ; 24 cm.
Contents: Part A. Isolation of nuclei and preparation of chromatin --
1. Procedures for minimizing protease activity during isolation of nuclei, chromatin, and the histones --
2. Isolation of nuclei from animal cells in culture --
3. Preparation of chromatin from animal tissues and cultured cells --
4. Methods for isolation of nuclei from cultured mammalian cells; conditional for preferential retention of selected histones --
5. Methods for isolation of nuclei from spermatozoa --
6. Manual enucleation of Xenopus oocytes --
7. Macro and micro methods for isolation and injection of cell components from polytene tissues --
8. Isolation of nuclei and chromatin from Phycomyces blakesleeanus --
Part B. Chromosomes --
9. Mammalian metaphase chromosomes --
10. Analytical techniques for isolated metaphase chromosome fractions --
11. Methods and mechanisms of chromosome banding --
12. cytological analysis of Drosophila polytene chromosomes --
13. Isolation and purification of nucleoli and nucleolar chromatin from mammalian cells --
14. Nucleolar proteins --
Part c. Fractionation and characterization of histones --
15. Electrophoretic fractionation of histones utilizing starch gels and sodium dodecyl sulfate-urea gels --
16. Resolution of histones by polyacrylamide gel electrophoresis in presence of nonionic detergents --
17. Polyacrylamide gel electrophoretic fractionation of histones --
Part D. Fractionation and characterization of nonhistone chromosomal proteins --
18. Affinity chromatography of DNA-binding proteins on DNA covalently attached to solid supports --
19. DNA-binding proteins --
20. Fractionation of nonhistone proteins by histone-affinity chromatography --
21. Fractionation of nonhistone chromosomal proteins --
22. Fractionation of chromosomal nonhistone proteins using chromatin-cellulose resins: purification of steroid hormone acceptor proteins --
23. Methods for assessing the binding of steroid hormones in nuclei --
24. Methods for assessing the binding of steroid hormones in nuclei and chromatin --
25. Proteins of nuclear ribonucleoprotein subcomplexes --
26. Isolation and characterization of ribonucleoprotein particles containing heterogeneous nuclear RNA --
27. Chromosomal association of an Epstein-Barr virus-associated nuclear antigen.
Series Title: Methods in cell biology, v. 17.
Responsibility: edited by Gary Stein and Janet Stein, Lewis J. Kleinsmith.

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