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E-mail Message: I thought you might be interested in this item at http://www.worldcat.org/oclc/33838261 Title: Clinical gene analysis and manipulation : tools, techniques, and troubleshooting Author: Janusz A Z Jankowski; Julia M Polak; Royal Postgraduate Medical School Publisher: Cambridge ; New York, NY, USA : Published in association with the Royal Postgraduate Medical School, University of London by Cambridge University Press, 1996. ISBN/ISSN: 0521473268 9780521473262 0521478960 9780521478960 OCLC:33838261

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Clinical gene analysis and manipulation : tools, techniques, and troubleshooting

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Clinical gene analysis and manipulation : tools, techniques, and troubleshooting

Author:	Janusz A Z Jankowski; Julia M Polak; Royal Postgraduate Medical School.
Publisher:	Cambridge ; New York, NY, USA : Published in association with the Royal Postgraduate Medical School, University of London by Cambridge University Press, 1996.
Series:	Postgraduate medical science.
Edition/Format:	Print book : EnglishView all editions and formats
Summary:	This practical compendium provides clinical scientists with an essential guide to the basic techniques of molecular medicine. It serves as a laboratory manual and a source of reference. It is suitable for those wishing to perform basic semi-quantitative experiments such as Northern or Southern blots and also those wishing to undertake more specialised genetic manipulations such as gene cloning, expression and Read more...
Rating:	(not yet rated) 0 with reviews - Be the first.
Subjects	Genetic engineering -- Laboratory manuals. Molecular genetics -- Laboratory manuals. Genetic engineering. View all subjects
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Genre/Form:	Laboratory manuals
Material Type:	Internet resource
Document Type:	Book, Internet Resource
All Authors / Contributors:	Janusz A Z Jankowski; Julia M Polak; Royal Postgraduate Medical School. Find more information about:
ISBN:	0521473268 9780521473262 0521478960 9780521478960
OCLC Number:	33838261
Description:	xviii, 459 pages : illustrations ; 26 cm.
Contents:	Part I. Introduction, Background and Essential Theory: 1. Introduction and applications J. Jankowski -- 2. History of molecular genetics R. Chinery -- 3. Molecules of gene expression (protein, RNA and DNA) R. Chinery -- Part II. Molecular Cell Tools: 4. Tissue analysis and morphological characterisation -- 5. Cellular analysis and cell culture -- 6. Chromosomal and molecular analysis -- 7. Gene manipulation F. Bedford and J. Jankowski -- Part III. Molecular Cell Techniques: 8. Cellular analysis -- Initiation and propagation of cultured cell lines I. Freshney -- Characterisation of cultured cell cines S. Kirkland and K. Henderson -- Cytoxicity assays in vitro R. Phillips and B. Boullier -- The production of monoclonal and polyclonal antibodies using synthetic peptides as immunogens T. RajKumar and B. Gullick -- 9. Tissue analysis -- Modern morphological techniques in molecular cell analysis (Immunocytochemistry autoradiography in situ hybridisation) L. Gordon and A. Bishop -- An overview of methodologies for assessing proliferation and cell death R. Dover -- 10. Chromosomal and molecular assays. 11. Assessing proteins and nucleic acids quantitatively and qualitatively by gel fractionation and related techniques -- 12. Extraction of protein, western blotting, special protein assays -- RIA and ELISA -- 13. Extraction of RNA and northern blot and RT PCR -- 14. Extraction of mammalian DNA, PCR, Southern blot and dot blot -- 15. Production of primers/probes J. Jankowski and F. Bedford -- 16. Molecular cytogenetic analysis of tumours and cell lines and its application in biology and medicine -- 17. Cytogenetics, FISH, allelle loss S. Jhanwar -- Analysis of DNA sequence polymorphisms and screening for point mutations A. Wright and J. Prosser -- 18. D. Gene manipulation -- 19. Molecular level -- 20. Basic cloning techniques -- 21. Purification of bacterial, plasmid or phage DNA cloning in prokaryotes and construction of recombinant DNA molecules S. Legon -- Creation and screening of cDNA libraries -- 22. A practical approach to cloning J. Goldenring and I. Modlin -- 23. Identification and isolation of novel DNA and cDna species F. Bedford and J. Jankowski -- 24. Manual and automated DNA and RNA sequencing M. Jones -- 25. Assessment of gene expression N. Bates and H. Hurst -- 26. Cellular level -- 27. Delivery of DNA into cells -- 28. Production of competent cells and cell transfection S. Castledon and R. Vile -- 29. Antisense RNA technology -- (Oligodeoxyribonucleotides, ribozymes and retroviruses) P.L. Iverson and I. Baysever -- 30. IV. Trouble-shooting: (i) Microbial contamination during cell culture K. Henderson. 31.(ii) Safety aspects of genetic manipulation work S. Legon -- 32. (iii) tissue storage and preparation J. Jankowski -- 33. (iv) Failed experiments -- general guidance J. Jankowski -- 34. (v) Selection of adequate controls J. Jankowski -- 35. (vi) Quality control J. Jankowski -- 36. V. Gene manipulation and applications to molecular medicine: 37. Inherited genetic disease and developmental disorders P. Buss -- 38. Oncology of solid tumours J. Jankowski -- 39. Oncology of haematogenous cancers S. Ridge -- 40. genetic therapies K. Sikora -- VI. Appendices: A. Selected bibliography -- B. Commercial suppliers -- 41. Equipment (quantitative gel analysis and gene manipulation) -- 42. Consumables -- 43. Solutions.
Series Title:	Postgraduate medical science.
Responsibility:	by Janusz A.Z. Jankowski, editor ; Julia M. Polak, deputy editor ; foreword, Sir David Weatherall.
More information:	Table of contents Publisher description

Abstract:

This helpful and practical compendium provides clinical scientists with an essential guide to the basic techniques of molecular medicine. It serves as a laboratory manual and a source of reference, Read more...

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'A copy of this book should be found in every biomedial science laboratory wishing to become involved in this highly relevant developing field.' J. Vaughan, British Journal of Biomedical Science

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