skip to content
Contributions of Intrinsically Disordered Regions of Proteins to the Assembly of Ribonucleoprotein Granules Preview this item
ClosePreview this item
Checking...

Contributions of Intrinsically Disordered Regions of Proteins to the Assembly of Ribonucleoprotein Granules

Author: David S W Protter; Roy Parker
Publisher: Ann Arbor : ProQuest Dissertations & Theses, 2017.
Dissertation: Ph. D. University of Colorado at Boulder 2017
Edition/Format:   Thesis/dissertation : Document : Thesis/dissertation : eBook   Computer File : English
Publication:Dissertation Abstracts International, 79-04B(E)
Summary:
Cells assemble large, non-membrane bound granules of protein and RNA, termed Ri- bonucleoprotein granules (RNP granules), often in response to a wide variety of cellular stresses. This behavior is conserved from yeast to mammals. Some RNP granules ap- pear important in the stress response, while others are important for proper organismal development, and still others for control of RNA degradation and transport.
Rating:

(not yet rated) 0 with reviews - Be the first.

Find a copy online

Links to this item

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Genre/Form: Theses
Academic theses
Material Type: Document, Thesis/dissertation, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: David S W Protter; Roy Parker
ISBN: 9780355542882 0355542889
OCLC Number: 1047524739
Language Note: English.
Notes: Source: Dissertation Abstracts International, Volume: 79-04(E), Section: B.
Advisors: Roy Parker Committee members: Loren Hough; Christopher Link; Amy Palmer; Marcelo Sousa.
Description: 1 electronic resource (112 pages)
Responsibility: David S.W Protter.

Abstract:

Cells assemble large, non-membrane bound granules of protein and RNA, termed Ri- bonucleoprotein granules (RNP granules), often in response to a wide variety of cellular stresses. This behavior is conserved from yeast to mammals. Some RNP granules ap- pear important in the stress response, while others are important for proper organismal development, and still others for control of RNA degradation and transport. Curiously, proteins found within granules are disproportionately likey to contain Intrinsically Dis- ordered Regions. Here, I show that those disordered regions can often drive higher order assembly in vitro and contribute to granule assembly in vivo. I found that these domains can make it easier for proteins to undergo a process known as Liquid-Liquid Phase Separa- tion in response to changes in ionic strength, wherein the protein of interest self-partitions into a concentrated liquid phase. The droplets that form mimic many of the behaviors of RNP granules in cells, such as recruitment of other IDR-containing proteins, assembly in response to RNA, and rapid exchange of contents with the surrounding medium. I also found that proteins that form these droplets tend to aggregate over time, turning the dynamic droplets into static structures.

Further, I identified several limitations to my in vitro model, most importantly the impairment of IDR-based phase separation in the presence of other proteins or cellular lysates. However, I also helped uncover the synergistic relationship between IDRs and the more well studied protein-protein and protein-RNA interactions that are important for granule assembly. I therefore propose an inclusive model of granule assembly which asserts that a wide variety of types of interactions are important, and that it is the sum-total of these interactions that determines whether or not a granule assembles.

Reviews

User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.
Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


Primary Entity

<http://www.worldcat.org/oclc/1047524739> # Contributions of Intrinsically Disordered Regions of Proteins to the Assembly of Ribonucleoprotein Granules
    a bgn:Thesis, pto:Web_document, schema:CreativeWork, schema:Book, schema:MediaObject ;
    bgn:inSupportOf "" ;
    library:oclcnum "1047524739" ;
    library:placeOfPublication <http://id.loc.gov/vocabulary/countries/miu> ;
    schema:author <http://experiment.worldcat.org/entity/work/data/5367807263#Person/protter_david_s_w> ; # David S. W. Protter
    schema:contributor <http://experiment.worldcat.org/entity/work/data/5367807263#Person/parker_roy> ; # Roy Parker
    schema:datePublished "2017" ;
    schema:description "Further, I identified several limitations to my in vitro model, most importantly the impairment of IDR-based phase separation in the presence of other proteins or cellular lysates. However, I also helped uncover the synergistic relationship between IDRs and the more well studied protein-protein and protein-RNA interactions that are important for granule assembly. I therefore propose an inclusive model of granule assembly which asserts that a wide variety of types of interactions are important, and that it is the sum-total of these interactions that determines whether or not a granule assembles."@en ;
    schema:description "Cells assemble large, non-membrane bound granules of protein and RNA, termed Ri- bonucleoprotein granules (RNP granules), often in response to a wide variety of cellular stresses. This behavior is conserved from yeast to mammals. Some RNP granules ap- pear important in the stress response, while others are important for proper organismal development, and still others for control of RNA degradation and transport. Curiously, proteins found within granules are disproportionately likey to contain Intrinsically Dis- ordered Regions. Here, I show that those disordered regions can often drive higher order assembly in vitro and contribute to granule assembly in vivo. I found that these domains can make it easier for proteins to undergo a process known as Liquid-Liquid Phase Separa- tion in response to changes in ionic strength, wherein the protein of interest self-partitions into a concentrated liquid phase. The droplets that form mimic many of the behaviors of RNP granules in cells, such as recruitment of other IDR-containing proteins, assembly in response to RNA, and rapid exchange of contents with the surrounding medium. I also found that proteins that form these droplets tend to aggregate over time, turning the dynamic droplets into static structures."@en ;
    schema:exampleOfWork <http://worldcat.org/entity/work/id/5367807263> ;
    schema:genre "Theses"@en ;
    schema:genre "Academic theses"@en ;
    schema:inLanguage "en" ;
    schema:name "Contributions of Intrinsically Disordered Regions of Proteins to the Assembly of Ribonucleoprotein Granules"@en ;
    schema:productID "1047524739" ;
    schema:url <http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqm&rft_dat=xri:pqdiss:10635323> ;
    schema:workExample <http://worldcat.org/isbn/9780355542882> ;
    wdrs:describedby <http://www.worldcat.org/title/-/oclc/1047524739> ;
    .


Related Entities

<http://experiment.worldcat.org/entity/work/data/5367807263#Person/parker_roy> # Roy Parker
    a schema:Person ;
    schema:familyName "Parker" ;
    schema:givenName "Roy" ;
    schema:name "Roy Parker" ;
    .

<http://experiment.worldcat.org/entity/work/data/5367807263#Person/protter_david_s_w> # David S. W. Protter
    a schema:Person ;
    schema:familyName "Protter" ;
    schema:givenName "David S. W." ;
    schema:name "David S. W. Protter" ;
    .

<http://worldcat.org/isbn/9780355542882>
    a schema:ProductModel ;
    schema:isbn "0355542889" ;
    schema:isbn "9780355542882" ;
    .

<http://www.worldcat.org/title/-/oclc/1047524739>
    a genont:InformationResource, genont:ContentTypeGenericResource ;
    schema:about <http://www.worldcat.org/oclc/1047524739> ; # Contributions of Intrinsically Disordered Regions of Proteins to the Assembly of Ribonucleoprotein Granules
    schema:dateModified "2019-06-14" ;
    void:inDataset <http://purl.oclc.org/dataset/WorldCat> ;
    .


Content-negotiable representations

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.