skip to content
The ELISA guidebook Preview this item
ClosePreview this item
Checking...

The ELISA guidebook

Author: J R Crowther
Publisher: Totowa, NJ : Humana Press, 2001.
Series: Methods in molecular biology (Clifton, N.J.), v. 149.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Highly sensitive, precise, and versatile, Enzyme Linked Immunosorbent Assay (ELISA) is now the predominant assay used in biomedical and agricultural research, as well as in applied science. In The ELISA Guidebook, John R. Crowther provides todayís premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and  Read more...
Rating:

(not yet rated) 0 with reviews - Be the first.

Subjects
More like this

Find a copy online

Links to this item

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Genre/Form: Electronic books
Additional Physical Format: Print version:
ELISA guidebook.
Totowa, NJ : Humana Press, 2000
(DLC) 99087692
(OCoLC)43060501
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: J R Crowther
ISBN: 9781592590490 0585365067 9780585365060 9780896037281 0896037282 0896039501 9780896039506 1592590497
OCLC Number: 1041031137
Notes: Previously published: 1995.
Description: 1 online resource (xi, 421 pages) : illustrations.
Contents: 1. Overview of ELISA in relation to other disciplines --
2. Systems in ELISA --
3. Stages in ELISA --
4. Titration of reagents --
5. Theoretical considerations --
6. Practical exercises --
7. Monoclonal antibodies --
8. Validation of diagnostic tests for infectious diseases --
9. Charting methods for internal quality control --
10. Immunochemical techniques --
11. Test questions.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 149.
Other Titles: Enzyme Linked Immunosorbent Assay
Responsibility: by John R. Crowther.

Abstract:

Highly sensitive, precise, and versatile, Enzyme Linked Immunosorbent Assay (ELISA) is now the predominant assay used in biomedical and agricultural research, as well as in applied science. In The ELISA Guidebook, John R. Crowther provides todayís premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. The author describes each method in great detail to ensure experimental success and includes advice on equipment choice, maintenance, and calibration. Wherever possible, helpful written explanations are provided along with copious diagrams. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research. --

Reviews

User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.
Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


Primary Entity

<http://www.worldcat.org/oclc/1041031137> # The ELISA guidebook
    a schema:MediaObject, schema:CreativeWork, schema:Book ;
    library:oclcnum "1041031137" ;
    library:placeOfPublication <http://id.loc.gov/vocabulary/countries/nju> ;
    library:placeOfPublication <http://experiment.worldcat.org/entity/work/data/5610599135#Place/totowa_nj> ; # Totowa, NJ
    schema:about <http://experiment.worldcat.org/entity/work/data/5610599135#Topic/diagnostic_parasitology> ; # Diagnostic parasitology
    schema:about <http://experiment.worldcat.org/entity/work/data/5610599135#Topic/enzyme_linked_immunosorbant_assay_methods> ; # Enzyme-Linked Immunosorbant Assay--methods
    schema:about <http://dewey.info/class/616.0756/e21/> ;
    schema:about <http://experiment.worldcat.org/entity/work/data/5610599135#Topic/enzyme_linked_immunosorbent_assay> ; # Enzyme-linked immunosorbent assay
    schema:alternateName "Enzyme Linked Immunosorbent Assay" ;
    schema:bookFormat schema:EBook ;
    schema:creator <http://experiment.worldcat.org/entity/work/data/5610599135#Person/crowther_j_r> ; # J. R. Crowther
    schema:datePublished "2001" ;
    schema:description "Highly sensitive, precise, and versatile, Enzyme Linked Immunosorbent Assay (ELISA) is now the predominant assay used in biomedical and agricultural research, as well as in applied science. In The ELISA Guidebook, John R. Crowther provides todayís premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. The author describes each method in great detail to ensure experimental success and includes advice on equipment choice, maintenance, and calibration. Wherever possible, helpful written explanations are provided along with copious diagrams. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research. --"@en ;
    schema:description "1. Overview of ELISA in relation to other disciplines -- 2. Systems in ELISA -- 3. Stages in ELISA -- 4. Titration of reagents -- 5. Theoretical considerations -- 6. Practical exercises -- 7. Monoclonal antibodies -- 8. Validation of diagnostic tests for infectious diseases -- 9. Charting methods for internal quality control -- 10. Immunochemical techniques -- 11. Test questions."@en ;
    schema:exampleOfWork <http://worldcat.org/entity/work/id/5610599135> ;
    schema:genre "Electronic books"@en ;
    schema:inLanguage "en" ;
    schema:isPartOf <http://experiment.worldcat.org/entity/work/data/5610599135#Series/methods_in_molecular_biology> ; # Methods in molecular biology ;
    schema:isPartOf <http://experiment.worldcat.org/entity/work/data/5610599135#Series/methods_in_molecular_biology_clifton_n_j> ; # Methods in molecular biology (Clifton, N.J.) ;
    schema:isSimilarTo <http://www.worldcat.org/oclc/43060501> ;
    schema:name "The ELISA guidebook"@en ;
    schema:productID "1041031137" ;
    schema:publication <http://www.worldcat.org/title/-/oclc/1041031137#PublicationEvent/totowa_nj_humana_press_2001> ;
    schema:publisher <http://experiment.worldcat.org/entity/work/data/5610599135#Agent/humana_press> ; # Humana Press
    schema:url <https://link.springer.com/10.1385/1592590497> ;
    schema:url <https://doi.org/10.1385/1592590497> ;
    schema:url <http://public.ebookcentral.proquest.com/choice/publicfullrecord.aspx?p=3036807> ;
    schema:url <http://web2.trentu.ca:2048/login?url=http://site.ebrary.com/lib/ocultrent/Doc?id=10181270> ;
    schema:url <http://www.library.yorku.ca/e/resolver/id/2527843> ;
    schema:url <http://www.library.yorku.ca/e/resolver/id/2527842> ;
    schema:url <https://ezproxy.library.dal.ca/login?url=http://public.ebookcentral.proquest.com/choice/publicfullrecord.aspx?p=3036807> ;
    schema:url <https://link.springer.com/openurl?genre=book&isbn=978-0-89603-728-1> ;
    schema:workExample <http://worldcat.org/isbn/9781592590490> ;
    schema:workExample <http://worldcat.org/isbn/9780896039506> ;
    schema:workExample <http://worldcat.org/isbn/9780896037281> ;
    schema:workExample <http://worldcat.org/isbn/9780585365060> ;
    wdrs:describedby <http://www.worldcat.org/title/-/oclc/1041031137> ;
    .


Related Entities

<http://experiment.worldcat.org/entity/work/data/5610599135#Person/crowther_j_r> # J. R. Crowther
    a schema:Person ;
    schema:familyName "Crowther" ;
    schema:givenName "J. R." ;
    schema:name "J. R. Crowther" ;
    .

<http://experiment.worldcat.org/entity/work/data/5610599135#Series/methods_in_molecular_biology> # Methods in molecular biology ;
    a bgn:PublicationSeries ;
    schema:hasPart <http://www.worldcat.org/oclc/1041031137> ; # The ELISA guidebook
    schema:name "Methods in molecular biology ;" ;
    .

<http://experiment.worldcat.org/entity/work/data/5610599135#Series/methods_in_molecular_biology_clifton_n_j> # Methods in molecular biology (Clifton, N.J.) ;
    a bgn:PublicationSeries ;
    schema:hasPart <http://www.worldcat.org/oclc/1041031137> ; # The ELISA guidebook
    schema:name "Methods in molecular biology (Clifton, N.J.) ;" ;
    .

<http://experiment.worldcat.org/entity/work/data/5610599135#Topic/diagnostic_parasitology> # Diagnostic parasitology
    a schema:Intangible ;
    schema:name "Diagnostic parasitology"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/5610599135#Topic/enzyme_linked_immunosorbant_assay_methods> # Enzyme-Linked Immunosorbant Assay--methods
    a schema:Intangible ;
    schema:name "Enzyme-Linked Immunosorbant Assay--methods"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/5610599135#Topic/enzyme_linked_immunosorbent_assay> # Enzyme-linked immunosorbent assay
    a schema:Intangible ;
    schema:name "Enzyme-linked immunosorbent assay"@en ;
    .

<http://web2.trentu.ca:2048/login?url=http://site.ebrary.com/lib/ocultrent/Doc?id=10181270>
    rdfs:comment "An electronic book accessible through the World Wide Web; click to view" ;
    .

<http://worldcat.org/isbn/9780585365060>
    a schema:ProductModel ;
    schema:isbn "0585365067" ;
    schema:isbn "9780585365060" ;
    .

<http://worldcat.org/isbn/9780896037281>
    a schema:ProductModel ;
    schema:isbn "0896037282" ;
    schema:isbn "9780896037281" ;
    .

<http://worldcat.org/isbn/9780896039506>
    a schema:ProductModel ;
    schema:isbn "0896039501" ;
    schema:isbn "9780896039506" ;
    .

<http://worldcat.org/isbn/9781592590490>
    a schema:ProductModel ;
    schema:isbn "1592590497" ;
    schema:isbn "9781592590490" ;
    .

<http://www.worldcat.org/oclc/43060501>
    a schema:CreativeWork ;
    rdfs:label "ELISA guidebook." ;
    schema:description "Print version:" ;
    schema:isSimilarTo <http://www.worldcat.org/oclc/1041031137> ; # The ELISA guidebook
    .


Content-negotiable representations

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.