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Enzymes : catalysis, kinetics and mechanisms

Author: N S Punekar
Publisher: Singapore : Springer, 2018.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to design enzyme experiments and  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Punekar, N. S.
Enzymes.
Singapore : Springer, 2018
(OCoLC)1033509252
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: N S Punekar
ISBN: 9789811307850 9811307857
OCLC Number: 1066238724
Description: 1 online resource (xxv, 562 pages) : illustrations (some color)
Contents: Part I: Enzyme catalysis --
a perspective --
Enzymes -Their place in Biology --
Enzymes --
Historical aspects --
Exploiting enzymes --
Technology --
On enzyme nomenclature and classification --
Hallmarks of an enzyme catalyst --
Origins of enzyme catalytic power --
Origins of enzyme catalytic power --
Which enzyme uses what trick? Some remarks --
Structure and catalysis: Conformational flexibility and protein motion --
Part II: Enzyme kinetic practice and measurements --
Chemical kinetics --
Fundamentals --
Concepts of equilibrium and steady-state --
ES complex and pre-steady-state kinetics --
Principles of enzyme assays --
Good kinetic practices --
Quantification of catalysis and measures of enzyme purity --
Henri-Michaelis-Menten equation --
More complex rate expressions --
Enzyme kinetic data --
Collection and analysis --
Part III: Elucidation of Kinetic mechanisms --
Approaches to kinetic mechanism --
Overview --
Analysis of initial velocity patterns --
Enzyme inhibition analyses --
Irreversible inhibitions --
Reversible inhibitions --
Alternate substrate (product) interactions --
pH Studies with enzymes --
Isotopes in enzymology --
Isotope exchanges at equilibrium --
Isotope effects in enzymology --
From kinetic data to mechanism and back --
Part IV: Chemical mechanisms and catalysis --
Chemical reactivity and molecular interactions --
Acid-base chemistry and catalysis --
Nucleophilic catalysis and covalent reaction intermediates --
Phosphoryl group chemistry and importance of ATP --
Enzymatic oxidation-reduction reactions --
Carboxylations and decarboxylations --
Electrophilic catalysis and amino acid transformations --
Integrating kinetic and chemical mechanisms --
A synthesis --
Part V: Frontiers in enzymology --
Regulation of enzyme activity --
In vitro versus in vivo --
Concept and consequences --
Future of enzymology --
An appraisal --
Closure --
Whither Enzymology.
Responsibility: N.S. Punekar.

Abstract:

The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers.  Read more...

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