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Genre/Form: | Thèses et écrits académiques |
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Material Type: | Thesis/dissertation |
Document Type: | Book |
All Authors / Contributors: |
Carlos Alexander Montoya Palacios; Philippe Leterme; Jean-Paul Lallès; Agrocampus Rennes |
OCLC Number: | 834777628 |
Notes: | 2007NSAR. |
Description: | 1 vol. (175 p.) : ill. ; 30 cm. |
Responsibility: | Carlos Alexander Montoya Palacios ; sous la dir. de Lallès, Jean-Paul et Leterme, P. |
Abstract:
The aim of this PhD thesis was to investigate the effects of purified phaseolin on protein digestin and intestinalmorphlogy and enzyme activites in the rat, and the susceptiblity of large numbers (43) of purified phaseolin varieties to proteolysis in vitro. Unheated phaseolin displayed low faecal digestibility (19-20 %), irrespective of the type of phaseolin studied. The type (S, T or I) of phaseolin, when unheated influenced the ileal concentration of phaseolin and of some of its digestion products. An incorporation level of phaseolin superior to 50 % of dietary protein affected intestinal morphology and enzymes activities and increased ileal losses of endogenous protein (pancreatic amylase and typsin). Thermal treatment obrogated these effects while improving drastically intestinal digestion and faecal digestibility (77-80 %) of phaseolin. The susceptibility of the 43 purified phaseolin varieties varied widely both without (11-27 %) and after (57-96 %) thermal treatment. We have confirmed from amino acid composition and in vitro proteolysis that sulphur amino acids are the main limiting amino acids in phaseolins. In conclusion, this work highlights the specific effects of phaseolin on the gut in vivo and their susceptibility to proteolysis in vitro. This work opens news perspectives for improving the nutritional quality of common bean for humans, based on selecting varieties with higher contents of limiting amino acids and/or higher susceptibility of phaseolin to proteolysis in vitro.
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