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Expansive growth of two glioblastoma stem-like cell lines is mediated by bFGF and not by EGF
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Expansive growth of two glioblastoma stem-like cell lines is mediated by bFGF and not by EGF

Author: Neža PodergajsNarve BrekkaBernhard RadlwimmerChristel Herold-MendeKrishna M Talasila; et al; All authors
Edition/Format:   Article : English
Publication:Articles from 7th Conference of experimental and translational oncology, [April, 20-24, 2013, Portorož] Vol. 47, no. 4 (dec. 2013), str. 330-337, II
Summary:
Background. Patient-derived glioblastoma (GBM) stem-like cells (GSCs) represent a valuable model for basic and therapeutic research. GSCs are usually propagated in serum-free Neural Basal medium supplemented with bFGF and EGF. Yet, the exact influence of these growth factors on GSCs is still unclear. Recently it was suggested that GBM stemlike cells with amplified EGFR should be cultured in stem cell medium without  Read more...
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Material Type: Internet resource
Document Type: Article, Internet Resource
All Authors / Contributors: Neža Podergajs; Narve Brekka; Bernhard Radlwimmer; Christel Herold-Mende; Krishna M Talasila; Katja Tiemann; Uroš Rajčević; Tamara Lah Turnšek; Rolf Bjerkvig; Hrvoje Miletic; et al
ISSN:1318-2099
OCLC Number: 868256043
In: Conference of experimental and translational oncology (7 : 2013 : Portorož
Notes: Izvleček v slov. na str. II.
Description: str. 330-337, II : Ilustr.
Responsibility: Neza Podergajs ... [et al.].

Abstract:

Background. Patient-derived glioblastoma (GBM) stem-like cells (GSCs) represent a valuable model for basic and therapeutic research. GSCs are usually propagated in serum-free Neural Basal medium supplemented with bFGF and EGF. Yet, the exact influence of these growth factors on GSCs is still unclear. Recently it was suggested that GBM stemlike cells with amplified EGFR should be cultured in stem cell medium without EGF, as the presence of EGF induced rapid loss of EGFR amplification. However, patient biopsies are usually taken into culture before their genomic profiles are defined. Thus, an important question remains whether GBM cells without EGFR amplification also can be cultured in stem cell medium without EGF.Meterials and methods. To address this question, we used two heterogeneous glioblastoma GSC lines (NCH421k and NCH644) that lack EGFR amplification.Results. Although both cell lines showed very low EGFR expression under standard growth conditions, bFGF stimulation induced higher expression of EGFR in NCH644. In both cell lines, expression of the stem cell markers nestin and CD133 was higher upon stimulation with bFGF compared to EGF. Importantly, bFGF stimulated the growth of both cell lines, whereas EGF had no effect. We verified that the growth stimulation by bFGF was either mediated by proliferation (NCH421k) or resistance to apoptosis (NCH644).Conclusions. We demonstrate that GSC cultures without EGFR amplification can be maintained and expanded with bFGF, while the addition of EGF has no significant effect and therefore can be omitted.

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Primary Entity<\/h3>
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<http:\/\/experiment.worldcat.org\/entity\/work\/data\/1773823669#Person\/herold_mende_christel<\/a>> # Christel Herold-Mende<\/span>\u00A0\u00A0\u00A0\u00A0a schema:Person<\/a> ;\u00A0\u00A0\u00A0schema:familyName<\/a> \"Herold-Mende<\/span>\" ;\u00A0\u00A0\u00A0schema:givenName<\/a> \"Christel<\/span>\" ;\u00A0\u00A0\u00A0schema:name<\/a> \"Christel Herold-Mende<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.<\/div>
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<http:\/\/experiment.worldcat.org\/entity\/work\/data\/1773823669#Person\/rajcevic_uros<\/a>> # Uro\u0161 Raj\u010Devi\u0107<\/span>\u00A0\u00A0\u00A0\u00A0a schema:Person<\/a> ;\u00A0\u00A0\u00A0schema:familyName<\/a> \"Raj\u010Devi\u0107<\/span>\" ;\u00A0\u00A0\u00A0schema:givenName<\/a> \"Uro\u0161<\/span>\" ;\u00A0\u00A0\u00A0schema:name<\/a> \"Uro\u0161 Raj\u010Devi\u0107<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.<\/div>
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/1773823669#Person\/talasila_krishna_m<\/a>> # Krishna M. Talasila<\/span>\u00A0\u00A0\u00A0\u00A0a schema:Person<\/a> ;\u00A0\u00A0\u00A0schema:familyName<\/a> \"Talasila<\/span>\" ;\u00A0\u00A0\u00A0schema:givenName<\/a> \"Krishna M.<\/span>\" ;\u00A0\u00A0\u00A0schema:name<\/a> \"Krishna M. Talasila<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.<\/div>
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/1773823669#Person\/tiemann_katja<\/a>> # Katja Tiemann<\/span>\u00A0\u00A0\u00A0\u00A0a schema:Person<\/a> ;\u00A0\u00A0\u00A0schema:familyName<\/a> \"Tiemann<\/span>\" ;\u00A0\u00A0\u00A0schema:givenName<\/a> \"Katja<\/span>\" ;\u00A0\u00A0\u00A0schema:name<\/a> \"Katja Tiemann<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.<\/div>
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<http:\/\/viaf.org\/viaf\/12144813879027058705<\/a>> # Bernhard Radlwimmer<\/span>\u00A0\u00A0\u00A0\u00A0a schema:Person<\/a> ;\u00A0\u00A0\u00A0schema:familyName<\/a> \"Radlwimmer<\/span>\" ;\u00A0\u00A0\u00A0schema:givenName<\/a> \"Bernhard<\/span>\" ;\u00A0\u00A0\u00A0schema:name<\/a> \"Bernhard Radlwimmer<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.<\/div>
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<http:\/\/viaf.org\/viaf\/70491958<\/a>> # Rolf Bjerkvig<\/span>\u00A0\u00A0\u00A0\u00A0a schema:Person<\/a> ;\u00A0\u00A0\u00A0schema:familyName<\/a> \"Bjerkvig<\/span>\" ;\u00A0\u00A0\u00A0schema:givenName<\/a> \"Rolf<\/span>\" ;\u00A0\u00A0\u00A0schema:name<\/a> \"Rolf Bjerkvig<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.<\/div>
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