skip to content
 The FhaB Prodomain of Bordetella Affects the Biogenesis... Preview this item
ClosePreview this item
Checking...

The FhaB Prodomain of Bordetella Affects the Biogenesis of Filamentous Hemaggluttinin, Influencing Bordetella Virulence

Author: School of Medicine, Department of Microbiology and Immunology; Cotter, Peggy; Noel, Christopher Robert
Publisher: University of North Carolina at Chapel Hill University of North Carolina at Chapel Hill 2012-12 2012
Dissertation: Thesis / Dissertation ETD
Edition/Format:   Thesis/dissertation : Thesis/dissertation : eBook : English
Summary:
Two-partner secretion (TPS) systems use beta-barrel proteins of the Omp85-TpsB superfamily to transport large exoproteins across the outer membranes of Gram-negative bacteria. The Bordetella FHA/FhaC proteins are prototypical of TPS systems in which the exoprotein contains a large C-terminal prodomain that is removed during translocation. Although it is known that the FhaB prodomain is required for FHA function in
Rating:

(not yet rated) 0 with reviews - Be the first.

Find a copy online

Links to this item

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Genre/Form: Dissertation
Material Type: Thesis/dissertation, Internet resource
Document Type: Internet Resource
All Authors / Contributors: School of Medicine, Department of Microbiology and Immunology; Cotter, Peggy; Noel, Christopher Robert
OCLC Number: 1105761964
Language Note: English

Abstract:

Two-partner secretion (TPS) systems use beta-barrel proteins of the Omp85-TpsB superfamily to transport large exoproteins across the outer membranes of Gram-negative bacteria. The Bordetella FHA/FhaC proteins are prototypical of TPS systems in which the exoprotein contains a large C-terminal prodomain that is removed during translocation. Although it is known that the FhaB prodomain is required for FHA function in vivo, its role in FHA maturation has remained mysterious. We show here that the FhaB prodomain is required for the extracellularly located mature C-terminal domain (MCD) of FHA to achieve its proper conformation. We show that the C-terminus of the prodomain is retained intracellularly and that sequences within the N-terminus of the prodomain are required for this intracellular localization. We also identify the MCD and prodomain N-terminus (PNT) as determinants of mature FHA release efficiency. Finally, we characterize subdomains at the C-terminus of the prodomain and their effects on FHA biogenesis and Bordetella virulence. Our data support a model in which the intracellularly located prodomain affects the final conformation of the extracellularly located MCD.

Doctor of Philosophy

Reviews

User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.
Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


Primary Entity

<http://www.worldcat.org/oclc/1105761964> # The FhaB Prodomain of Bordetella Affects the Biogenesis of Filamentous Hemaggluttinin, Influencing Bordetella Virulence
    a schema:Book, schema:CreativeWork, bgn:Thesis ;
    bgn:inSupportOf "Thesis / Dissertation ETD" ;
    library:oclcnum "1105761964" ;
    schema:contributor <http://experiment.worldcat.org/entity/work/data/9356703102#Agent/cotter_peggy> ; # Cotter, Peggy
    schema:contributor <http://experiment.worldcat.org/entity/work/data/9356703102#Agent/school_of_medicine_department_of_microbiology_and_immunology> ; # School of Medicine, Department of Microbiology and Immunology
    schema:creator <http://experiment.worldcat.org/entity/work/data/9356703102#Agent/noel_christopher_robert> ; # Noel, Christopher Robert
    schema:datePublished "2012" ;
    schema:datePublished "2012/12" ;
    schema:description "Doctor of Philosophy" ;
    schema:description "Two-partner secretion (TPS) systems use beta-barrel proteins of the Omp85-TpsB superfamily to transport large exoproteins across the outer membranes of Gram-negative bacteria. The Bordetella FHA/FhaC proteins are prototypical of TPS systems in which the exoprotein contains a large C-terminal prodomain that is removed during translocation. Although it is known that the FhaB prodomain is required for FHA function in vivo, its role in FHA maturation has remained mysterious. We show here that the FhaB prodomain is required for the extracellularly located mature C-terminal domain (MCD) of FHA to achieve its proper conformation. We show that the C-terminus of the prodomain is retained intracellularly and that sequences within the N-terminus of the prodomain are required for this intracellular localization. We also identify the MCD and prodomain N-terminus (PNT) as determinants of mature FHA release efficiency. Finally, we characterize subdomains at the C-terminus of the prodomain and their effects on FHA biogenesis and Bordetella virulence. Our data support a model in which the intracellularly located prodomain affects the final conformation of the extracellularly located MCD." ;
    schema:exampleOfWork <http://worldcat.org/entity/work/id/9356703102> ;
    schema:genre "Dissertation" ;
    schema:inLanguage "en" ;
    schema:name "The FhaB Prodomain of Bordetella Affects the Biogenesis of Filamentous Hemaggluttinin, Influencing Bordetella Virulence" ;
    schema:productID "1105761964" ;
    schema:publication <http://www.worldcat.org/title/-/oclc/1105761964#PublicationEvent/university_of_north_carolina_at_chapel_hilluniversity_of_north_carolina_at_chapel_hill2012_122012> ;
    schema:publisher <http://experiment.worldcat.org/entity/work/data/9356703102#Agent/university_of_north_carolina_at_chapel_hill> ; # University of North Carolina at Chapel Hill
    schema:url <https://cdr.lib.unc.edu/concern/dissertations/7h149q30j> ;
    wdrs:describedby <http://www.worldcat.org/title/-/oclc/1105761964> ;
    .


Related Entities

<http://experiment.worldcat.org/entity/work/data/9356703102#Agent/cotter_peggy> # Cotter, Peggy
    a bgn:Agent ;
    schema:name "Cotter, Peggy" ;
    .

<http://experiment.worldcat.org/entity/work/data/9356703102#Agent/noel_christopher_robert> # Noel, Christopher Robert
    a bgn:Agent ;
    schema:name "Noel, Christopher Robert" ;
    .

<http://experiment.worldcat.org/entity/work/data/9356703102#Agent/school_of_medicine_department_of_microbiology_and_immunology> # School of Medicine, Department of Microbiology and Immunology
    a bgn:Agent ;
    schema:name "School of Medicine, Department of Microbiology and Immunology" ;
    .

<http://experiment.worldcat.org/entity/work/data/9356703102#Agent/university_of_north_carolina_at_chapel_hill> # University of North Carolina at Chapel Hill
    a bgn:Agent ;
    schema:name "University of North Carolina at Chapel Hill" ;
    .

<http://www.worldcat.org/title/-/oclc/1105761964>
    a genont:InformationResource, genont:ContentTypeGenericResource ;
    schema:about <http://www.worldcat.org/oclc/1105761964> ; # The FhaB Prodomain of Bordetella Affects the Biogenesis of Filamentous Hemaggluttinin, Influencing Bordetella Virulence
    schema:dateModified "2019-07-02" ;
    void:inDataset <http://purl.oclc.org/dataset/WorldCat> ;
    void:inDataset <http://purl.oclc.org/dataset/WorldCat/DigitalCollectionGateway> ;
    .


Content-negotiable representations

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.