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FOXO transcription factors : methods and protocols

Author: Wolfgang Link
Publisher: New York, N.Y. : Humana Press : Springer, [2018] ©2019
Series: Methods in molecular biology (Clifton, N.J.), v. 1890.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

Chapters guide readers through biochemical and molecular methods, imaging approaches to monitor the subcellular localization of FOXO factors, omics and bioinformatics approaches, different animal  Read more...

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Genre/Form: Laboratory Manual
Electronic books
Laboratory manuals
Additional Physical Format: Print version:
FOXO transcription factors.
New York, N.Y : Springer, ©2019
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Wolfgang Link
ISBN: 9781493989003 1493989006
OCLC Number: 1062398136
Description: 1 online resource (xii, 270 pages) : illustrations (some color)
Contents: Introduction to FOXO biology / Wolfgang Link --
Characterization of MicroRNAs regulating FOXO expression / Doug Hanniford and Eva Hernando --
Identification of transcription factor-binding sites in the mouse FOXO1 promoter / Christopher P. Cardozo --
Investigating mechanisms that control ubiquitin-mediated DAF-16/FOXO protein turnover / Thomas Heimbucher and Coleen T. Murphy --
Phosphorylation of FOXO proteins as a key mechanism to regulate their activity / Alba Orea-Soufi, David Davila, Maria Salazar-Roa, Maria de Mar Lorente, and Guillermo Velasco --
Methodological approach for the evaluation of FOXO as a positive regulator of antioxidant genes / Maria Monsalve, Ignacio Prieto, Andreza Fabro de Bem, and Yolanda Olmos --
Characterization of FOXO acetylation / Shang Yao, Zimam Mahmud, Nikoleta Sachini, Sathid Aimjongjun, Paula Saavedra-Garcia, and Eric W-F Lam --
Transcriptional activity of FOXO transcription factors measured by luciferase assays / Antonia Avila-Flores, Javier Arranz-Nicolas, and Isabel Merida --
Monitoring the transcriptional activity of FOXO transcription factors by analyzing their target genes / Vanessa Henriques, Susana Machado, Wolfgang Link, and Bibiana I. Ferreira --
Using ChIP-based approaches to characterize FOXO recruitment to its target promoters / Neeraj Kumar and Arnab Mukhopadhyay --
RNAi-mediated silencing of FOXO factors / Priyankar Sanphui, Akash Saha, and Subhas Chandra Biswas --
Immunofluorescence analysis by confocal microscopy for detecting endogenous FOXO / Francisco Castillo, Thomas A. Mackenzie, and Bastien Cautain --
High-throughput image-based screening to identify chemical compounds capable of activating FOXO / Marta Barradas, Wolfgang Link, Diego Megias, and Pablo J. Fernandez-Marcos --
Image-based identification of chemical compounds capable of trapping FOXO in the cell nucleus / Susana Machado, Catarina Raposo, Bibiana I. Ferreira, and Wolfgang Link --
Quantifying tissue-specific overexpression of FOXO in Drosophila via mRNA fluorescence in situ hybridization using branched DNA probe technology / Anna C. Blice-Baum, Georg Vogler, Meera C. Viswanathan, Bosco Trinh, Worawan B. Limpitikul, and Anthony Cammarato --
Genome-wide analysis for identifying FOXO protein-binding sites / Dong-Ju Shin, Pujan Joshi, Dong-Guk Shin, and Li Wang --
Mathematical modeling of nuclear trafficking of FOXO transcription factors / Bradford E. Peercy and Martin F. Schneider --
Following transcriptome to uncover FOXO biological functions / Raymond Liang, Vijay Menon, and Saghi Ghaffari --
Hydra as model to determine the role of FOXO in longevity / Thomas C.G. Bosch --
Genetic ablation of FOXO in mice to investigate its physiological role / Hwanhee Oh and Jihye Paik --
Analysis of FOXO3 gene polymorphisms associated with human longevity / Timothy A. Donlon, Philip M.C. Davy, and Bradley J. Willcox --
Analysis of FOXO3a gene polymorphism associated with asthma / Tejas Shah and Kalpana Joshi.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1890.
Responsibility: edited by Wolfgang Link.

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