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Fundamental laboratory approaches for biochemistry and biotechnology

Author: Alexander J Ninfa; David P Ballou; Marilee Benore
Publisher: Hoboken, NJ : John Wiley, ©2010.
Edition/Format:   Print book : English : 2nd edView all editions and formats
Summary:
This book was written as a biochemistry laboratory text for advanced students in biochemistry and other life sciences. It provides a logical framework on how to approach research problems and how to conduct and evaluate scientific research.
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Genre/Form: Laboratory manuals
Laboratory Manual
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Alexander J Ninfa; David P Ballou; Marilee Benore
ISBN: 9780470087664 0470087668
OCLC Number: 420027217
Description: xix, 458 pages : illustrations
Contents: Getting started in scientific research --
Basic procedures in the biochemistry laboratory --
Spectroscopic methods --
Quantification of protein concentration --
Chromatography --
Gel electrophoresis of proteins --
Overview of protein purification --
Subcellular fractionation --
Isolation and characterization of an enzyme-alkaline phosphatase from escherichia coli --
Enzyme kinetics --
Ligand binding --
Enzymatic methods of analysis --
Recombinant DNA techniques --
Polymerase chain reaction (PCR) technology --
Using the computer and the internet for biochemical research and communication.
Responsibility: Alexander J. Ninfa, David P. Ballou, Marilee Benore.
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Abstract:

Suitable for lower-level and upper-level courses and can also be used for some first-year graduate work. Dedicated to developing research skills in students, allowing them to learn  Read more...

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