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Genotype phenotype coupling : methods and protocols

Author: Stefan Zielonka; Simon Krah
Publisher: New York : Humana Press, [2020] ©2010
Series: Methods in molecular biology (Clifton, N.J.), v. 2070.
Edition/Format:   eBook : Document : EnglishView all editions and formats

This volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display.


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Genre/Form: Electronic books
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Stefan Zielonka; Simon Krah
ISBN: 9781493998531 1493998536
OCLC Number: 1124958004
Description: 1 online resource : illustrations (some color).
Contents: 1. Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for TobramycinNandi Zhou, Rongfeng Cai, and Xuyan Han2. Affitins: Ribosome Display for Selection of Aho7c-based Affinity ProteinsValentina Kalichuk, Stanimir Kambarev, Ghislaine Behar, Benjamin Chalopin, Axelle Renodon-Corniere, Barbara Mouratou, and Frederic Pecorari3. cDNA Display: A Stable and Simple Genotype-phenotype Coupling Using a Cell-free Translation SystemHidenao Arai, Shigefumi Kumachi, and Naoto Nemoto4. cDNA Display of Disulfide-containing Peptide Library and in vitro EvolutionTai Kubo and Mohammed Naimuddin 5. Rapid Antigen and Antibody-like Molecule Discovery by Staphylococcal Surface DisplayMarco Cavallari6. Restriction-free Construction of a Phage-presented Very Short Macrocyclic Peptide LibraryValentin Jakob, Saskia Helmsing, Michael Hust, and Martin Empting7. In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical PropertiesJohn Steven, Obinna C. Ubah, Magdalena Buschhaus, Marina Kovaleva, Laura Ferguson, Andrew J. Porter, and Caroline J. Barelle8. Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens Esther V. Wenzel, Kristian D. R. Roth, Giulio Russo, Viola Fuhner, Saskia Helmsing, Andre Frenzel, and Michael Hust9. Assessing Antibody Specificity in Human Serum Using Deep Sequence-coupled BiopanningKathryn M. Frietze, Susan B. Core, Alexandria Linville, Bryce Chackerian, and David S. Peabody10. Isolation of Antigen-specific VHH Single Domain Antibodies by Combining Animal Immunization with Yeast Surface DisplayLukas Roth, Simon Krah, Janina Klemm, Ralf Guenther, Lars Toleikis, Michael Busch, Stefan Becker, and Stefan Zielonka 11. Selection and Characterization of Anti-idiotypic Shark Antibody Domains Doreen Koenning, Stefan Zielonka, Anna Kaempffe, Sebastian Jager, Harald Kolmar, and Christian Schroeter12. Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP ExpressionSteffen C. Hinz, Adrian Elter, Julius Grzeschik, Jan Habermann, Bastian Becker, and Harald Kolmar13. Methods for Construction of Yeast Display Libraries of Four-Domain T-cell ReceptorsFlavio Sadio, Gerhard Stadlmayr, Katja Eibensteiner, Katharina Stadlbauer, Florian Ruker, and Gordana Wozniak-Knopp14. Isolation of Tailor-made Antibody Fragments from Yeast-displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell SortingAnna Kaempffe, Sebastian Jager, Doreen Koenning, Harald Kolmar, and Christian Schroeter15. Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene RepertoiresSebastian Jager, Simon Krah, Doreen Koenning, Janis Rosskopf, Stephan Dickgiesser, Nicolas Rasche, Harald Kolmar, Stefan Hecht, and Christian Schroeter16. Rapid Generation of Chicken Immune Libraries for Yeast Surface DisplayJan P. Bogen, Julius Grzeschik, Simon Krah, Stefan Zielonka, and Harald Kolmar17. Ligand Engineering via Yeast Surface Display and Adherent Cell PanningLawrence A. Stern, Patrick S. Lown, and Benjamin J. Hackel18. Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces Cerevisiae Using Inefficient Ribosomal SkippingCarlos A. Cruz-Teran, Kaitlyn Bacon, and Balaji M. Rao19. Chemical Modification of the Yeast Cell Surface Allows the Switch between Display and Soluble Secretion of Full-length AntibodiesSimon Krah, Ralf Gunther, Stefan Becker, Stefan Zielonka, and Laura Rhiel20. Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Geno-Phenotype Coupling Transposon VectorsKaren Berg, Vanessa Nicole Schafer, Natalie Bartnickiand, and Joern Stitz 21. Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult to Drug TargetsZachary R. Crook, Gregory P. Sevilla, Andrew J. Mhyre, and James M. Olson22. Engineering Antibodies on the Surface of CHO CellsAnnalee W. Nguyen, Kevin Le, and Jennifer A. Maynard23. Single B Cell Cloning and Production of Rabbit Monoclonal AntibodiesJuliet Rashidian and Joshua Lloyd
Series Title: Methods in molecular biology (Clifton, N.J.), v. 2070.
Responsibility: edited by Stefan Zielonka and Simon Krah.


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