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Germline stem cells

Author: Steven X Hou; Shree Ram Singh
Publisher: Totowa, NJ : Humana Press, ©2008.
Series: Methods in molecular biology (Clifton, N.J.), v. 450.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Publication:Springer Protocols
Summary:
The knowledge gained by studying germline stem cells, the source of human and animal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors. In Germline Stem Cells, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell  Read more...
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Genre/Form: Electronic books
Laboratory Manuals
Additional Physical Format: Print version:
Germline stem cells.
Totowa, N.J. : Humana Press, ©2008
(DLC) 2007938052
(OCoLC)191760107
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Steven X Hou; Shree Ram Singh
ISBN: 1603272143 9781603272148
OCLC Number: 226379635
Language Note: English.
Description: 1 online resource (xii, 273 pages) : illustrations (some color).
Contents: pt. I. Identification and regulation of germline stem cells in model organisms. 1. The development of germline stem cells in Drosophila / David A. Dansereau and Paul Lasko. 2. Analysis of the C. elegans germline stem cell region / Sarah L. Crittenden and Judith Kimble . 3 Immunohistological techniques for studying the Drosophila male germline stem cell / Shree Ram Singh and Steven X. Hou. 4 Genetic tools used for cell lineage tracing and gene manipulation in Drosophila germline stem cells / Wei Liu and Steven X. Hou. 5. Structural polarity and dynamics of male germline stem cells in an insect (milkweed bug Oncopeltus fasciatus) / David C. Dorn and August Dorn. 6. High-resolution light microscopic characterization of spermatogonia / Hélio Chiarini-Garcia and Marvin L. Meistrich. 7. Identification and characterization of spermatogonial subtypes and their expansion in whole mounts and tissue sections from primate testes / Jens Ehmcke and Stefan Schlatt. 8. Epigenetic control in male germ cells : a transillumination-assisted microdissection method for the analysis of developmentally regulated events / Durga Prasad Mishra and / Paolo Sassone-Corsi. 9. GDNF maintains mouse spermatogonial stem cells in vivo and in vitro / Hannu Sariola and Tiina Immonen --
pt. II. In vitro culture and application of germline stem cells. 10 Ectopic grafting of mammalian testis tissue into mouse hosts / Ina Dobrinski and Rahul Rathi. 11. Spermatogonial stem cell transplantation, testicular function, and restoration of male fertility in mice / Derek J. McLean. 12. Isolating highly pure rat spermatogonial stem cells in culture / F. Kent Hamra [and others]. 13. Deriving mouse spermatogonial stem cell lines / Ilaria Falciatori [and others]. 14. Production of knockdown rats by lentiviral transduction of embryos with short hairpin RNA transgenes / Christina Tenenhaus Dann and David L. Garbers. .15. Testicular germ cell tumors in mice : new ways to study a genetically complex trait / Jason D. Heaney and Joseph H. Nadeau. 16. Study origin of germ cells and formation of new primary follicles in adult human and rat ovaries / Antonin Bukovsky [and others].
Series Title: Methods in molecular biology (Clifton, N.J.), v. 450.
Responsibility: edited by Steven X. Hou, Shree Ram Singh.
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Abstract:

In this comprehensive and cutting-edge book, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate,  Read more...

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From the reviews:"This book provides background information and protocols for identifying, isolating, characterizing, and manipulating ... germline stem cells. ... This book is written for basic Read more...

 
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