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Green fluorescent protein : properties, applications, and protocols

Author: Martin Chalfie; Steven Kain
Publisher: Hoboken, N.J : Wiley-Interscience, 2011.
Series: Methods of biochemical analysis, v. 47
Edition/Format:   eBook : Document : English : 2nd edView all editions and formats
Summary:
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species.
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Genre/Form: Laboratory manuals
Additional Physical Format: Erscheint auch als:
Druck-Ausgabe
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Martin Chalfie; Steven Kain
ISBN: 0471739499 9780471739494 0471739480 9780471739487 0471736821 9780471736820 9780471453284 0471453285
OCLC Number: 1039170208
Description: Online Ressource (xv, 443 Seiten, 24 unnumbered Seiten of plates) : Illustrationen (some color).
Contents: Discovery of green fluorescent protein / Osamu Shimomura. Photons for reporting molecular events : green fluorescent protein and four luciferase systems / J. Woodland Hastings and James G. Morin. Biochemical and physical properties of green fluorescent protein / William W. Ward. The three-dimensional structure of green fluorescent protein and its implications for function and design / George N. Phillips, Jr. Molecular biology and mutation of green fluorescent protein / David A. Zacharias, Roger Y. Tsien. Discovery and properties of GFP-like proteins from nonbioluminescent anthozoa / Konstantin A. Lukyanov [and others]. Evolution of function and color in GFP-like proteins / Mikhail V. Matz, Yulii A. Labas, and Juan Ugalde. The uses of green fluorescent protein in prokaryotes / Raphael H. Valdivia, Brendan P. Cormack, and Stanley Falkow. The uses of green fluorescent protein in yeasts / Amy L. Hitchcock, Jason A. Kahana, and Pamela A. Silver. Uses of GFP in Caenorhabditis elegans / Oliver Hobert and Paula Loria. Green fluorescent protein applications in Drosophila / Tulle Hazelrigg and Jennifer H. Mansfield. The uses of green fluorescent protein in plants / Jim Haseloff and Kirby R. Siemering. Uses of GFP in transgenic vertebrates / Sean Magason [and others]. The uses of green fluorescent protein in mammalian cells / Theresa H. Ward and Jennifer Lippincott-Schwartz. Practical considerations for use of reef coral fluorescent proteins in mammalian cells : applications in fluorescent microscopy and flow cytometry / Yu Fang [and others]. Pharmaceutical applications of GFP and RCFP / Nicola Bevan and Stephen Rees. Reassembled GFP : detecting protein-protein interactions and protein expression patterns / Thomas J. Magliery and Lynne Regan.
Series Title: Methods of biochemical analysis, v. 47
Responsibility: edited by Martin Chalfie, Steven R. Kain.

Abstract:

Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species.

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"...a magnificent text that will immensely benefit students and scientists from many biomedical areas." (Annals of Biomedical Engineering, August 2006) "Any researcher, graduate student or educator Read more...

 
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