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High Performance Liquid Chromatography

Author: Heinz Engelhardt
Publisher: Berlin, Heidelberg : Springer Berlin Heidelberg, 1979.
Series: Chemical Laboratory Practice; Anleitungen für die chemische Laboratoriumspraxis.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Modern liquid column chromatography (LC) has developed rapidly since 1969 to become a standard method of separation. If the statisticians are to be believed, the recent growth of LC has been the most specta­ cular development in analytical chemistry and has not yet abated be­ cause its vast potential for application remains to be fully exploit­ ed. Significant factors contributing to this continued rise are the  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Heinz Engelhardt
ISBN: 9783642670640 3642670644 9783642670664 3642670660
OCLC Number: 858929694
Description: 1 online resource.
Contents: I. Chromatographic Processes --
References Chapter I --
II. Fundamentals of Chromatography --
A. Retention --
B. Linear Flow Rate, Porosity, Permeability --
C. Band Broadening --
D. Resolution --
E. Dependence of Band Broadening on Flow Rate --
F. Band Broadening and Particle Size --
G. Extra-Column Band Broadening --
H. Optimum Analysis Conditions and Analysis Time --
I. Selection of a Suitable Column --
References Chapter II --
III. Equipment for HPLC --
A. Solvent Reservoir --
Degassing of the Eluent --
B. Pumps --
C. Damping of the Pulsations --
D. Sample Introduction --
E. The Column --
F. Thermostating --
G. Measurement of the Flow Rate --
H. Fraction Collectors --
I. Recorders --
J. Instrumentation for Gradient Elution --
K. Safety Measures --
References Chapter III --
IV. Detectors --
A. UV Detectors --
B. Differential Refractometer --
C. Microadsorption Detector --
D. Transport Detector (Flame Ionization Detector) --
E. Fluorescence Detector --
F. Other Detectors --
G. Comparison of the Important Detectors --
H. Reaction Detectors --
References Chapter IV --
V. Stationary Phases --
A. Packing Materials for Adsorption and Partition Chromatography --
B. Chemically Modified Supports --
C. Ion Exchangers --
D. Stationary Phases for Exclusion Chromatography --
References Chapter V --
VI. Adsorption Chromatography --
I. Polar Stationary Phases --
II. Nonpolar Stationary Phases --
III. The General Elution Problem --
IV. Applications of Adsorption Chromatography --
References Chapter VI --
VII. Partition Chromatography --
A. Introduction --
B. Supports and Liquid Phases --
C. Properties of the Column --
D. Applications --
E. Ion-pair Chromatography --
References Chapter VII --
VIII. Ion-Exchange Chromatography --
A. Principle --
B. Ion Exchange Materials --
C. Characterization of the Ion Exchangers --
D. Optimizing a Separation --
E. Applications --
References Chapter VIII --
IX. Exclusion Chromatography. Gel Permeation Chromatography --
A. Introduction --
B. Basics of Exclusion Chromatography --
C. Stationary Phases for Exclusion Chromatography --
D. Applications of Exclusion Chromatography --
References Chapter IX --
X. Selection of the Separation System --
References Chapter X --
XI. Special Techniques --
A. Preparative Chromatography --
B. Qualitative Analysis --
C. Quantitative Analysis --
D. Trace Analysis --
References Chapter XI --
XII. Purification of Solvents --
References Chapter XII.
Series Title: Chemical Laboratory Practice; Anleitungen für die chemische Laboratoriumspraxis.
Responsibility: by Heinz Engelhardt.

Abstract:

Modern liquid column chromatography (LC) has developed rapidly since 1969 to become a standard method of separation. Also, whether the capillary columns recent- ly advocated with great enthusiasm for  Read more...

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