skip to content
Covid-19 virus
COVID-19 Resources

Reliable information about the coronavirus (COVID-19) is available from the World Health Organization (current situation, international travel). Numerous and frequently-updated resource results are available from this WorldCat.org search. OCLC’s WebJunction has pulled together information and resources to assist library staff as they consider how to handle coronavirus issues in their communities.

Image provided by: CDC/ Alissa Eckert, MS; Dan Higgins, MAM
High throughput protein expression and purification : methods and protocols Preview this item
ClosePreview this item
Checking...

High throughput protein expression and purification : methods and protocols

Author: Sharon A Doyle
Publisher: Totowa, N.J. : Humana Press ; [London] : [Springer, distributor], ©2009.
Series: Methods in molecular biology (Clifton, N.J.), 498.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:
Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In 'High Throughput Protein Expression and Purification', leading scientists detail the most successful protocols currently in use, including high throughput cloning schemes.
Rating:

(not yet rated) 0 with reviews - Be the first.

Subjects
More like this

Find a copy online

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Sharon A Doyle
ISBN: 9781588298799 1588298795 9781597451963 1597451967
OCLC Number: 241042860
Description: xii, 322 pages : illustrations (some color) ; 26 cm.
Contents: High-throughput protein production (HTTP) : a review of enabling technologies to expedite protein production / Jim Koehn and Ian Hunt --
Designing experiments for high-throughput protein expression / Stephen P. Chambers and Susanne E. Swalley --
Gateway cloning for protein expression / Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath --
Flexi vector cloning / Paul G. Blommel [and others] --
The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning / Nick S. Berrow, David Alderton, and Raymond J. Owens --
The polymerase incomplete primer extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis / Heath E. Klock and Scott A. Lesley --
A family of LIC vectors for high-throughput cloning and purification of proteins / William H. Eschenfeldt [and others] --
"System 48" high-throughput cloning and protein expression analysis / James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart --
Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni²-particles / Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery --
E. coli and insect cell expression, automated purification and quantitative analysis / StephenP. Chambers [and others] --
Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli / Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh --
PHB-intein-mediated protein purification strategy / Alison R. Gillies, Mahmoud Reza Banki, and David W. Wood --
High-throughput biotinylation of proteins / Brian K. Kay, Sang Thai, and Veronica V. Volgina --
High-throughput insect cell protein expression applications / Mirjam Buchs [and others] --
High-throughput protein expression using cell-free system / Kalavathy Sitaraman and Deb K. Chatterjee --
The production of glycoproteins by transient expression in mammalian cells / Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens --
High-throughput expression and detergent screening of integral membrane proteins / Said Eshaghi --
Cell-free expression for nanolipoprotein particles : building a high-throughput membrane protein solubility platform / Jenny A. Cappuccio [and others] --
Expression and purification of soluble His₆-tagged TEV protease / Joseph E. Tropea, Scott Cherry, and David S. Waugh --
High-throughput protein concentration and buffer exchange : comparison of ultrafiltration and ammonium sulfate precipitation / Priscilla A. Moore and Vladimir Kery.
Series Title: Methods in molecular biology (Clifton, N.J.), 498.
Responsibility: edited by Sharon A. Doyle.
More information:

Abstract:

In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning  Read more...

Reviews

Editorial reviews

Publisher Synopsis

From the reviews:"The book consists 20 chapters, which cover methods in protein expression and purification summarized by leading experts in this area from USA, UK, and Switzerland. ... Numerous Read more...

 
User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.
Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


\n\n

Primary Entity<\/h3>\n
<http:\/\/www.worldcat.org\/oclc\/241042860<\/a>> # High throughput protein expression and purification : methods and protocols<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Book<\/a>, schema:CreativeWork<\/a> ;\u00A0\u00A0\u00A0\nlibrary:oclcnum<\/a> \"241042860<\/span>\" ;\u00A0\u00A0\u00A0\nlibrary:placeOfPublication<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Place\/totowa_n_j<\/a>> ; # Totowa, N.J.<\/span>\n\u00A0\u00A0\u00A0\nlibrary:placeOfPublication<\/a> <http:\/\/id.loc.gov\/vocabulary\/countries\/nju<\/a>> ;\u00A0\u00A0\u00A0\nlibrary:placeOfPublication<\/a> <http:\/\/dbpedia.org\/resource\/London<\/a>> ; # London<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/dewey.info\/class\/572.636\/e22\/<\/a>> ;\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteomik<\/a>> ; # Proteomik<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteine<\/a>> ; # Proteine<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/reinigungsverfahren<\/a>> ; # Reinigungsverfahren<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteins_isolation_&_purification<\/a>> ; # Proteins--isolation & purification<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteines_separation<\/a>> ; # Prot\u00E9ines--S\u00E9paration<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/biokemi<\/a>> ; # Biokemi<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteins_genetics<\/a>> ; # Proteins--genetics<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteiner<\/a>> ; # Proteiner<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteins_separation_methodology<\/a>> ; # Proteins--Separation--Methodology<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteomics_methods<\/a>> ; # Proteomics--methods<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/gene_expression<\/a>> ; # Gene Expression<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/genexpression<\/a>> ; # Genexpression<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/trennverfahren<\/a>> ; # Trennverfahren<\/span>\n\u00A0\u00A0\u00A0\nschema:bookFormat<\/a> bgn:PrintBook<\/a> ;\u00A0\u00A0\u00A0\nschema:contributor<\/a> <http:\/\/viaf.org\/viaf\/58910071<\/a>> ; # Sharon Ann Doyle<\/span>\n\u00A0\u00A0\u00A0\nschema:copyrightYear<\/a> \"2009<\/span>\" ;\u00A0\u00A0\u00A0\nschema:datePublished<\/a> \"2009<\/span>\" ;\u00A0\u00A0\u00A0\nschema:description<\/a> \"Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In \'High Throughput Protein Expression and Purification\', leading scientists detail the most successful protocols currently in use, including high throughput cloning schemes.<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\nschema:description<\/a> \"High-throughput protein production (HTTP) : a review of enabling technologies to expedite protein production \/ Jim Koehn and Ian Hunt -- Designing experiments for high-throughput protein expression \/ Stephen P. Chambers and Susanne E. Swalley -- Gateway cloning for protein expression \/ Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath -- Flexi vector cloning \/ Paul G. Blommel [and others] -- The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning \/ Nick S. Berrow, David Alderton, and Raymond J. Owens -- The polymerase incomplete primer extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis \/ Heath E. Klock and Scott A. Lesley -- A family of LIC vectors for high-throughput cloning and purification of proteins \/ William H. Eschenfeldt [and others] -- \"System 48\" high-throughput cloning and protein expression analysis \/ James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart -- Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni\u00B2-particles \/ Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery -- E. coli and insect cell expression, automated purification and quantitative analysis \/ StephenP. Chambers [and others] -- Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli \/ Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh -- PHB-intein-mediated protein purification strategy \/ Alison R. Gillies, Mahmoud Reza Banki, and David W. Wood -- High-throughput biotinylation of proteins \/ Brian K. Kay, Sang Thai, and Veronica V. Volgina -- High-throughput insect cell protein expression applications \/ Mirjam Buchs [and others] -- High-throughput protein expression using cell-free system \/ Kalavathy Sitaraman and Deb K. Chatterjee -- The production of glycoproteins by transient expression in mammalian cells \/ Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens -- High-throughput expression and detergent screening of integral membrane proteins \/ Said Eshaghi -- Cell-free expression for nanolipoprotein particles : building a high-throughput membrane protein solubility platform \/ Jenny A. Cappuccio [and others] -- Expression and purification of soluble His\u2086-tagged TEV protease \/ Joseph E. Tropea, Scott Cherry, and David S. Waugh -- High-throughput protein concentration and buffer exchange : comparison of ultrafiltration and ammonium sulfate precipitation \/ Priscilla A. Moore and Vladimir Kery.<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\nschema:exampleOfWork<\/a> <http:\/\/worldcat.org\/entity\/work\/id\/810213746<\/a>> ;\u00A0\u00A0\u00A0\nschema:inLanguage<\/a> \"en<\/span>\" ;\u00A0\u00A0\u00A0\nschema:isPartOf<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Series\/methods_in_molecular_biology_clifton_n_j<\/a>> ; # Methods in molecular biology (Clifton, N.J.) ;<\/span>\n\u00A0\u00A0\u00A0\nschema:isPartOf<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Series\/methods_in_molecular_biology<\/a>> ; # Methods in molecular biology ;<\/span>\n\u00A0\u00A0\u00A0\nschema:name<\/a> \"High throughput protein expression and purification : methods and protocols<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\nschema:productID<\/a> \"241042860<\/span>\" ;\u00A0\u00A0\u00A0\nschema:publication<\/a> <http:\/\/www.worldcat.org\/title\/-\/oclc\/241042860#PublicationEvent\/totowa_n_j_humana_press_london_springer_distributor_2009<\/a>> ;\u00A0\u00A0\u00A0\nschema:publisher<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Agent\/springer_distributor<\/a>> ; # [Springer, distributor]<\/span>\n\u00A0\u00A0\u00A0\nschema:publisher<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Agent\/humana_press<\/a>> ; # Humana Press<\/span>\n\u00A0\u00A0\u00A0\nschema:url<\/a> <http:\/\/catdir.loc.gov\/catdir\/toc\/fy0906\/2008931181.html<\/a>> ;\u00A0\u00A0\u00A0\nschema:url<\/a> <http:\/\/bvbr.bib-bvb.de:8991\/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016790949&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA<\/a>> ;\u00A0\u00A0\u00A0\nschema:url<\/a> <http:\/\/www.springerprotocols.com\/BookToc\/doi\/10.1007\/978-1-59745-196-3<\/a>> ;\u00A0\u00A0\u00A0\nschema:workExample<\/a> <http:\/\/worldcat.org\/isbn\/9781588298799<\/a>> ;\u00A0\u00A0\u00A0\nschema:workExample<\/a> <http:\/\/worldcat.org\/isbn\/9781597451963<\/a>> ;\u00A0\u00A0\u00A0\numbel:isLike<\/a> <http:\/\/bnb.data.bl.uk\/id\/resource\/GBA896549<\/a>> ;\u00A0\u00A0\u00A0\nwdrs:describedby<\/a> <http:\/\/www.worldcat.org\/title\/-\/oclc\/241042860<\/a>> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n\n

Related Entities<\/h3>\n
<http:\/\/dbpedia.org\/resource\/London<\/a>> # London<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Place<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"London<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/dewey.info\/class\/572.636\/e22\/<\/a>>\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Agent\/humana_press<\/a>> # Humana Press<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nbgn:Agent<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Humana Press<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Agent\/springer_distributor<\/a>> # [Springer, distributor]<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nbgn:Agent<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"[Springer, distributor]<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Place\/totowa_n_j<\/a>> # Totowa, N.J.<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Place<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Totowa, N.J.<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Series\/methods_in_molecular_biology<\/a>> # Methods in molecular biology ;<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nbgn:PublicationSeries<\/a> ;\u00A0\u00A0\u00A0\nschema:hasPart<\/a> <http:\/\/www.worldcat.org\/oclc\/241042860<\/a>> ; # High throughput protein expression and purification : methods and protocols<\/span>\n\u00A0\u00A0\u00A0\nschema:name<\/a> \"Methods in molecular biology ;<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Series\/methods_in_molecular_biology_clifton_n_j<\/a>> # Methods in molecular biology (Clifton, N.J.) ;<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nbgn:PublicationSeries<\/a> ;\u00A0\u00A0\u00A0\nschema:hasPart<\/a> <http:\/\/www.worldcat.org\/oclc\/241042860<\/a>> ; # High throughput protein expression and purification : methods and protocols<\/span>\n\u00A0\u00A0\u00A0\nschema:name<\/a> \"Methods in molecular biology (Clifton, N.J.) ;<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/biokemi<\/a>> # Biokemi<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Biokemi<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/gene_expression<\/a>> # Gene Expression<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Gene Expression<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/genexpression<\/a>> # Genexpression<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Genexpression<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteine<\/a>> # Proteine<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Proteine<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteiner<\/a>> # Proteiner<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Proteiner<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteines_separation<\/a>> # Prot\u00E9ines--S\u00E9paration<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Prot\u00E9ines--S\u00E9paration<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteins_genetics<\/a>> # Proteins--genetics<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Proteins--genetics<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteins_isolation_&_purification<\/a>> # Proteins--isolation & purification<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Proteins--isolation & purification<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteins_separation_methodology<\/a>> # Proteins--Separation--Methodology<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:hasPart<\/a> <http:\/\/id.loc.gov\/authorities\/subjects\/sh85107676<\/a>> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Proteins--Separation--Methodology<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteomics_methods<\/a>> # Proteomics--methods<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Proteomics--methods<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/proteomik<\/a>> # Proteomik<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Proteomik<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/reinigungsverfahren<\/a>> # Reinigungsverfahren<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Reinigungsverfahren<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Topic\/trennverfahren<\/a>> # Trennverfahren<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Trennverfahren<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/id.loc.gov\/vocabulary\/countries\/nju<\/a>>\u00A0\u00A0\u00A0\u00A0a \nschema:Place<\/a> ;\u00A0\u00A0\u00A0\ndcterms:identifier<\/a> \"nju<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/viaf.org\/viaf\/58910071<\/a>> # Sharon Ann Doyle<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Person<\/a> ;\u00A0\u00A0\u00A0\nschema:familyName<\/a> \"Doyle<\/span>\" ;\u00A0\u00A0\u00A0\nschema:givenName<\/a> \"Sharon Ann<\/span>\" ;\u00A0\u00A0\u00A0\nschema:givenName<\/a> \"Sharon A.<\/span>\" ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Sharon Ann Doyle<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/worldcat.org\/isbn\/9781588298799<\/a>>\u00A0\u00A0\u00A0\u00A0a \nschema:ProductModel<\/a> ;\u00A0\u00A0\u00A0\nschema:isbn<\/a> \"1588298795<\/span>\" ;\u00A0\u00A0\u00A0\nschema:isbn<\/a> \"9781588298799<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/worldcat.org\/isbn\/9781597451963<\/a>>\u00A0\u00A0\u00A0\u00A0a \nschema:ProductModel<\/a> ;\u00A0\u00A0\u00A0\nschema:isbn<\/a> \"1597451967<\/span>\" ;\u00A0\u00A0\u00A0\nschema:isbn<\/a> \"9781597451963<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/www.worldcat.org\/title\/-\/oclc\/241042860<\/a>>\u00A0\u00A0\u00A0\u00A0a \ngenont:InformationResource<\/a>, genont:ContentTypeGenericResource<\/a> ;\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/www.worldcat.org\/oclc\/241042860<\/a>> ; # High throughput protein expression and purification : methods and protocols<\/span>\n\u00A0\u00A0\u00A0\nschema:dateModified<\/a> \"2019-03-18<\/span>\" ;\u00A0\u00A0\u00A0\nvoid:inDataset<\/a> <http:\/\/purl.oclc.org\/dataset\/WorldCat<\/a>> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/www.worldcat.org\/title\/-\/oclc\/241042860#PublicationEvent\/totowa_n_j_humana_press_london_springer_distributor_2009<\/a>>\u00A0\u00A0\u00A0\u00A0a \nschema:PublicationEvent<\/a> ;\u00A0\u00A0\u00A0\nschema:location<\/a> <http:\/\/dbpedia.org\/resource\/London<\/a>> ; # London<\/span>\n\u00A0\u00A0\u00A0\nschema:location<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Place\/totowa_n_j<\/a>> ; # Totowa, N.J.<\/span>\n\u00A0\u00A0\u00A0\nschema:organizer<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Agent\/springer_distributor<\/a>> ; # [Springer, distributor]<\/span>\n\u00A0\u00A0\u00A0\nschema:organizer<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/810213746#Agent\/humana_press<\/a>> ; # Humana Press<\/span>\n\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n\n

Content-negotiable representations<\/p>\n