skip to content
High-throughput protein production and purification : methods and protocols Preview this item
ClosePreview this item
Checking...

High-throughput protein production and purification : methods and protocols

Author: Renaud Vincentelli
Publisher: New York, NY : Humana Press, 2019.
Series: Methods in molecular biology (Clifton, N.J.), v. 2025.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein  Read more...

Rating:

(not yet rated) 0 with reviews - Be the first.

Subjects
More like this

Find a copy online

Links to this item

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: (OCoLC)1091844519
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Renaud Vincentelli
ISBN: 9781493996247 149399624X
OCLC Number: 1110728028
Description: 1 online resource (xvi, 537 pages) : illustrations (some color).
Contents: Part 1 General Procedures1) HTP cloning 2) HTP generation of truncation and mutation libraries3) HTP protein production in Escherichia coli 4) HTP Production of Protein Complexes by Co-expression in Escherichia coli 5) HTP protein complex reconstitution in Escherichia coli6) HTP protein production in alternative bacterial system7) HTP protein production in Yeast8) HTP protein production and protein complexes in Baculovirus9) HTP protein production and protein complexes in Mammalian cells10) HTP protein production in plants11) HTP protein expression in bacterial Cell Free system12) HTP protein expression in eukaryotic Cell Free system13) HTP refolding of proteins14) HTP production of binders15) HTP production of antibodies16) HTP Modification of strains for improved protein expression (CHO, HEK, E. coli)Part 2 Case studies17) HTP production of membrane proteins 18) HTP membrane protein expression in bacterial Cell Free system19) HTP production of kinases20) HTP production of small cysteine-rich proteins (VENOMICS)21) HTP production of transcription factors22) HTP Protein production for drug discoveryPart 3 HTP micro-characterizations of proteins23) Analytical SEC- MALLS24) Differential Scanning Fluorimetry25) Native mass spectrometry26) Protein-protein interaction (Pull-down/Hold-up)27) Protein-DNA (SELEX)28) Analytical Electron microscopy
Series Title: Methods in molecular biology (Clifton, N.J.), v. 2025.
Responsibility: edited by Renaud Vincentelli.

Reviews

User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.
Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


Primary Entity

<http://www.worldcat.org/oclc/1110728028> # High-throughput protein production and purification : methods and protocols
    a schema:CreativeWork, schema:Book, schema:MediaObject ;
    library:oclcnum "1110728028" ;
    library:placeOfPublication <http://id.loc.gov/vocabulary/countries/nyu> ;
    schema:about <http://experiment.worldcat.org/entity/work/data/9377823383#Topic/proteins_synthesis> ; # Proteins--Synthesis
    schema:about <http://experiment.worldcat.org/entity/work/data/9377823383#Topic/combinatorial_chemistry> ; # Combinatorial chemistry
    schema:about <http://dewey.info/class/572.645/e23/> ;
    schema:bookFormat schema:EBook ;
    schema:datePublished "2019" ;
    schema:editor <http://experiment.worldcat.org/entity/work/data/9377823383#Person/vincentelli_renaud> ; # Renaud Vincentelli
    schema:exampleOfWork <http://worldcat.org/entity/work/id/9377823383> ;
    schema:genre "Laboratory manuals"@en ;
    schema:genre "Electronic books"@en ;
    schema:inLanguage "en" ;
    schema:isPartOf <http://worldcat.org/issn/1064-3745> ; # Methods in molecular biology (Clifton, N.J.) ;
    schema:isPartOf <http://worldcat.org/issn/1940-6029> ; # Methods in molecular biology,
    schema:isSimilarTo <http://www.worldcat.org/oclc/1091844519> ;
    schema:name "High-throughput protein production and purification : methods and protocols"@en ;
    schema:productID "1110728028" ;
    schema:url <https://doi.org/10.1007/978-1-4939-9624-7> ;
    schema:workExample <http://dx.doi.org/10.1007/978-1-4939-9624-7> ;
    schema:workExample <http://worldcat.org/isbn/9781493996247> ;
    umbel:isLike <http://bnb.data.bl.uk/id/resource/GBB9D8276> ;
    wdrs:describedby <http://www.worldcat.org/title/-/oclc/1110728028> ;
    .


Related Entities

<http://experiment.worldcat.org/entity/work/data/9377823383#Person/vincentelli_renaud> # Renaud Vincentelli
    a schema:Person ;
    schema:familyName "Vincentelli" ;
    schema:givenName "Renaud" ;
    schema:name "Renaud Vincentelli" ;
    .

<http://experiment.worldcat.org/entity/work/data/9377823383#Topic/combinatorial_chemistry> # Combinatorial chemistry
    a schema:Intangible ;
    schema:name "Combinatorial chemistry"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/9377823383#Topic/proteins_synthesis> # Proteins--Synthesis
    a schema:Intangible ;
    schema:name "Proteins--Synthesis"@en ;
    .

<http://worldcat.org/isbn/9781493996247>
    a schema:ProductModel ;
    schema:isbn "149399624X" ;
    schema:isbn "9781493996247" ;
    .

<http://worldcat.org/issn/1064-3745> # Methods in molecular biology (Clifton, N.J.) ;
    a bgn:PublicationSeries ;
    schema:hasPart <http://www.worldcat.org/oclc/1110728028> ; # High-throughput protein production and purification : methods and protocols
    schema:issn "1064-3745" ;
    schema:name "Methods in molecular biology (Clifton, N.J.) ;" ;
    .

<http://worldcat.org/issn/1940-6029> # Methods in molecular biology,
    a bgn:PublicationSeries ;
    schema:hasPart <http://www.worldcat.org/oclc/1110728028> ; # High-throughput protein production and purification : methods and protocols
    schema:issn "1940-6029" ;
    schema:name "Methods in molecular biology," ;
    .

<http://www.worldcat.org/oclc/1091844519>
    a schema:CreativeWork ;
    schema:isSimilarTo <http://www.worldcat.org/oclc/1110728028> ; # High-throughput protein production and purification : methods and protocols
    .


Content-negotiable representations

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.