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Histone variants : methods and protocols

Author: Genevieve Almouzni; Guillermo A Orsi
Publisher: New York, NY : Humana Press : Springer, [2018] ©2018
Series: Methods in molecular biology (Clifton, N.J.), v. 1832.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

This volume compiles methodologies used for detailed studies of histone variants, from their basic properties to their functional roles in chromatin and as vectors of epigenetic information.

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Genre/Form: Laboratory Manual
Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Genevieve Almouzni; Guillermo A Orsi
ISBN: 9781493986637 1493986635 9781493986620 1493986627
OCLC Number: 1047602677
Description: 1 online resource (xii, 359 pages) : illustrations (some color)
Contents: Methods for preparing nucleosomes containing histone variants / Tomoya Kujirai, Yasuhiro Arimura, Risa Fujita, Naoki Horikoshi, Shinichi Machida, and Hitoshi Kurumizaka --
Characterization of posttranslational modifications on histone variants / Francisco Saavedra, Sebastián Marty-Lombardi, and Alejandra Loyola --
Purification of histone variant-interacting chaperone complexes / Hideaki Tagami --
Detection of histone modification dynamics during the cell cycle by MS-based proteomics / Moritz Carl Völker-Albert, Andreas Schmidt, Teresa K. Barth, Ignasi Forne, and Axel Imhof --
Histone native chromatin immunoprecipitation / Alicia Alonso, Emily Bernstein, and Dan Hasson --
How to tackle challenging ChIP-seq, with long-range cross-linking, using ATRX as an example / Julia Truch, Jelena Telenius, Douglas R. Higgs, and Richard J. Gibbons --
time-ChIP : a method to determine long-term locus-specific nucleosome inheritance / Wojciech Siwek, Mariluz Gómez-Rodríguez, Daniel Sobral, Ivan R. Corrêa Jr, and Lars E.T. Jansen --
MINCE-seq : mapping in vivo nascent chromatin with EdU and sequencing / Srinivas Ramachandran and Steven Henikoff --
RChIP-seq : chromatin-associated RNA sequencing in developmentally staged mouse testes / Tatiana A. Soboleva and David J. Tremethick --
Bioinformatic analysis of nucleosome and histone variant positioning / Jakub Mieczkowski and Michael Y. Tolstorukov --
Imaging newly synthesized and old histone variant dynamics dependent on chaperones using the SNAP-tag system / Júlia Torné, Guillermo A. Orsi, Dominique Ray-Gallet, and Geneviève Almouzni --
Real-time de novo deposition of centromeric histone-associated proteins using the auxin-inducible degradation system / Sebastian Hoffmann and Daniele Fachinetti --
Live imaging of parental histone variant dynamics in UVC-damaged chromatin / Juliette Dabin, Anna Fortuny, Sandra Piquet, and Sophie E. Polo --
CRISPR/Cas9 gene editing of human gistone H2A variant H2AX and macroH2A / Justin W.C. Leung, Lara E. Emery, and Kyle M. Miller --
Studying the evolution of histone variants using phylogeny / Antoine Molaro and Ines A. Drinnenberg --
Characterization of post-meiotic male germ cell genome organizational states / Jérôme Govin, Sophie Barral, Yuichi Morozumi, Naghmeh Hoghoughi, Thierry Buchou, Sophie Rousseaux, and Saadi Khochbin --
Animal model for genetic analysis of multi-gene families : cloning and transgenesis of large tandemly repeated histone gene clusters / Michael P. Meers, Mary Leatham-Jensen, Taylor J.R. Penke, Daniel J. McKay, Robert J. Duronio, and A. Gregory Matera --
Imaging and quantitation of assembly dynamics of the centromeric histone H3 variant CENP-A in Drosophila melanogaster spermatocytes by immunofluorescence and fluorescence in-situ hybridization (Immuno-FISH) / Caitríona M. Collins and Elaine M. Dunleavy --
Probing the function of oncohistones using mutant transgenes and knock-in mutations / Dong Fang, Heping Wang, and Zhiguo Zhang.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1832.
Responsibility: edited by Guillermo A. Orsi, Geneviève Almouzni.

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