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Imaging, manipulation and optogenetics in zebrafish

Author: Itia Amandine Favre-Bulle
Publisher: Cham : Springer, [2018] ©2018
Series: Springer theses.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
The work described here investigates the advantages and limitations of using laser light for the deep in-vivo illumination and micromanipulation of the neuronal system in zebrafish. To do so, it combines and develops novel optical methods such as optogenetics, light sheet microscopy and optical micromanipulation. It also demonstrates, for the first time, that directional and focused laser beams can successfully be  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Favre-Bulle, Itia Amandine.
Imaging, manipulation and optogenetics in zebrafish.
Cham : Springer, [2018]
(OCoLC)1041498373
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Itia Amandine Favre-Bulle
ISBN: 9783319962504 3319962507 9783319962511 3319962515
OCLC Number: 1048428935
Notes: "Doctoral thesis accepted by the University of Queensland, Brisbane, Australia.
Description: 1 online resource : illustrations (some color)
Contents: Intro; Supervisor's Foreword; Reference; Abstract; Publications Related to this Thesis; Acknowledgements; Contents; Abbreviations; 1 Introduction; References; 2 Light Scattering in Brain Tissue Using Monte Carlo Method; 2.1 Monte Carlo Method and Our Model; 2.1.1 Introduction to Monte Carlo; 2.1.2 Complexity of Models and the Monte Carlo Solution; 2.2 Details of Monte Carlo Method Code Writing for Scattering in Brain Tissue; 2.3 Results of Monte Carlo Method; 2.4 Conclusion; References; 3 Scattering in Zebrafish Brain for Optogenetics. 3.1 Description of Set-Up to Study Light Scattering in Brain Tissue3.1.1 Introduction to Spatial Light Modulators (SLM); 3.1.2 Experimental Set-Up for Studies of Scattering; 3.2 Experimental Measurements; 3.2.1 Experimental Procedures; 3.2.2 Measurements; 3.3 Comparing Monte Carlo Method with Experimental Measurements; 3.4 Conclusions; References; 4 Optical Systems to Decode Brain Activity; 4.1 Fluorescent Proteins and the Development of GECIs; 4.2 Imaging Neuronal Activity; 4.3 The Advantages of Combining Optical Systems; 4.4 Building of the Optogenetics System. 4.4.1 Presentation of the Optogenetics System4.4.2 Set-Up Improvements Using SLM Flexibility; 4.4.3 Stimulation of Specific Brain Regions and Deduction of Brain Circuitry; 4.5 Conclusions; References; 5 Investigation of Optical Properties of Otoliths with Optical Trapping; 5.1 Description of the Vestibular System; 5.1.1 Necessity of a Non-invasive System; 5.1.2 Structure of the Anterior Otolith; 5.2 Optical Trapping for Otolith Manipulation; 5.2.1 Introduction to Optical Trapping; 5.2.2 Investigation of the Possibility of Otolith Manipulation; 5.2.3 Manipulation of Free Otolith. 5.3 Force Measurement with Optical Trapping5.3.1 Introduction to Light Deflection Method; 5.3.2 Experimental Otolith Scanning and Force Measurements; 5.4 Discussion; References; 6 Optical Manipulation of Otoliths In-Vivo; 6.1 Experimental Set-Up; 6.2 Measuring Behavioural Responses as a Result of Otolith Manipulation; 6.2.1 Experimental Procedures; 6.2.2 Behavioural Responses to Otolith Trapping; 6.2.3 Tail and Eyes Movements in the Literature; 6.3 Set Up Improvements for Calcium Imaging; 6.3.1 Details of ETL Functionning and Performances. 6.4 Calcium Imaging: Mapping Out Responses to Acceleration6.4.1 Past Relevant Studies on Brain Activity in Zebrafish; 6.4.2 Experimental Procedure; 6.4.3 Preliminary Results; 6.5 Discussion; References; 7 Conclusion.
Series Title: Springer theses.
Responsibility: Itia Amandine Favre-Bulle.

Abstract:

It also demonstrates, for the first time, that directional and focused laser beams can successfully be used to target large objects at considerable depth in a living organism to exert purely optical  Read more...

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