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Immune mediators in cancer : methods and protocols

Author: Ivana Vancurova; Yan Zhu
Publisher: New York : Humana Press, 2020.
Series: Methods in molecular biology (Clifton, N.J.), v. 2108.
Edition/Format:   eBook : Document : English

This book provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches that are used to quantify immune mediators and analyze  Read more...


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Genre/Form: Electronic books
Additional Physical Format: Print version:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Ivana Vancurova; Yan Zhu
ISBN: 9781071602478 1071602470
OCLC Number: 1137547340
Contents: Part I: Detection of Immune Mediators in Cancer Cells and Tissues 1. A Useful Guide for Analysis of Immune Mediators in Cancer by Fluorochrome (Luminex) Technique Maria Faresjoe 2. Analysis of Circulating HMGB1 in Human Serum Weiqiang Chen, Guoqiang Bao, Lin Zhao, and Haichao Wang 3. Analysis of IL-22 and Th22 Cells by Flow Cytometry in Systemic Lupus Erythematosus Zhuang Ye, Ling Zhao, Qi Gao, Yanfang Jiang, Zhenyu Jiang, and Cong-Qiu Chu 4. Application of Immunohistochemistry in Basic and Clinical Studies Aihua Li and Dong-Hua Yang 5. Interleukin-1 (IL-1) Immunohistochemistry Assay in Oral Squamous Cell Carcinoma Takaaki Kamatani 6. Luminex xMAP Assay to Quantify Cytokines in Cancer Patient Serum Helena Kupcova Skalnikova, Katerina Vodickova Kepkova, and Petr Vodicka 7. Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence Herui Wang, Ryan L. Pangilinan, and Yan Zhu Part II: Cytokine Bioassays 8. Analysis of IFNg-Induced Migration of Ovarian Cancer Cells Bijaya Gaire, Mohammad M. Uddin, Yue Zou, and Ivana Vancurova 9. Interleukin-8-Induced Invasion Assay in Triple Negative Breast Cancer Cells Mohammad M. Uddin, Bijaya Gaire, Betsy Deza, and Ivana Vancurova 10. Interleukin-8 Induces Proliferation of Ovarian Cancer Cells in 3D Spheroids Mohammad M. Uddin, Bijaya Gaire, and Ivana Vancurova 11. Detection of Ferroptosis by BODIPY (TM) 581/591 C11 Alejandra M. Martinez, Ahryun Kim, and Wan Seok Yang 12. Methods for Studying TNF -Induced Autophagy Sheyda Najafi, Ehab M. Abo-Ali, and Vikas V. Dukhande 13. Isolation of Antibody Binders to MISIIR from a Phage Display Library by Sorting Andy Qingan Yuan 14. Measuring Chimeric Antigen Receptor T Cells (CAR T Cells) Activation by Coupling Intracellular Cytokine Staining with Flow Cytometry Chong Xu and Yibo Yin 15. Analysis of Interleukin-4-Induced Class Switch Recombination in Mouse Myeloma CH12F3-2 Cells Wenjun Wu, Zhihui Xiao, Deon Buritis, and Vladimir Poltoratsky 16. Single-Stranded DNA Aptamers against TNF and Their Potential Applications Shao Tao, Song Pingfang, Xiaowei Zhang, Lingshu Zhang, and Cong-Qiu Chu 17. Probing Metabolic Changes in IFN -Treated Ovarian Cancer Cells Pritpal Kaur, Shreya Nagar, Madhura Bhagwat, Mohammad Uddin, Yan Zhu, and Ales Vancura Part III: Expression and Regulation of Immune Mediators in Cancer Cells 18. Immunoblotting Analysis of Intracellular PD-L1 Levels in Interferon-g-Treated Ovarian Cancer Cells Stably Transfected with Bcl3 shRNA Sveta Padmanabhan, Yue Zou, and Ivana Vancurova 19. Flow Cytometry Analysis of Surface PD-L1 Expression Induced by IFNg and Romidepsin in Ovarian Cancer Cells Sveta Padmanabhan, Yue Zou, and Ivana Vancurova 20. Analysis of PD-L1 Transcriptional Regulation in Ovarian Cancer Cells by Chromatin Immunoprecipitation Yue Zou, Sveta Padmanabhan, and Ivana Vancurova 21. Real-Time PCR Assay for the Analysis of Alternative Splicing of Immune Mediators in Cancer Ruizhi Wang, Md. Faruk Hossain, Jovan Mirkovic, Samuel Sabzanov, and Matteo Ruggiu 22. Combined Single-Cell Measurement of Cytokine mRNA and Protein in Immune Cells Julian J. Freen-van Heeren, Benoit P. Nicolet, and Monika C. Wolkers 23. Microscopic Methods for Analysis of Macrophage-Induced Tunneling Nanotubes Kiersten P. Carter, Jeffrey E. Segall, and Dianne Cox 24. Optogenetics: Rho GTPases Activated by Light in Living Macrophages Maren Hulsemann, Polina V. Verkhusha, Peng Guo, Veronika Miskolci, Dianne Cox, and Louis Hodgson Part IV: Bioinformatics and Genome Editing Approaches 25. High-Throughput RNA interference Screen Targeting Synthetic-Lethal Gain-of-Function of Oncogenic Mutant TP53 in Triple Negative Breast Cancer Susumu Rokudai 26. Discovering Transcription Factor Non-Coding RNA Targets Using ChIP-Seq Analysis Vitalay Fomin and Carol Prives 27. Phylogenetic Analyses of Chemokine Receptors from Sequence Retrieval to Phylogenetic Trees Juan C. Santos 28. Generation of IL17RB Knockout Cell Lines Using CRISPR/Cas9-Based Genome Editing Olivia Hu, Alessandro Provvido, and Yan Zhu 29. Engineering Mutation Clones in Mammalian Cells with CRISPR/Cas9 Zijun Huo, Jian Tu, Dung-Fang Lee, and Ruiying Zhao
Series Title: Methods in molecular biology (Clifton, N.J.), v. 2108.
Responsibility: edited by Ivana Vancurova and Yan Zhu.


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