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Introduction to light microscopy : tips and tricks for beginners

Author: Dee Lawlor
Publisher: Cham, Switzerland : Springer, [2019]
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This book offers a beginners guide to using light microscopes. It begins with a brief introduction to the physics of optics, which will give the reader a basic grasp of the behaviors of light. In turn, each part of the microscope is explained using clear and simple English, together withdetailed photographs and diagrams. The reader will learn the function, care and correct use of each part. A troubleshooting section  Read more...
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Genre/Form: Electronic books
Textbooks
Additional Physical Format: (OCoLC)1061816189
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Dee Lawlor
ISBN: 9783030053932 3030053938
OCLC Number: 1103467645
Description: 1 online resource
Contents: Intro; Dedication; Preface; Acknowledgements; About the Book; Contents; About the Author; 1: Introduction; 1.1 History; 1.2 The Importance of Glass in Microscopy; 1.3 What Classifies as a Microscope?; 1.4 Why We Find Microscopes Appealing; References; 2: The Advantages of Learning Traditional Microscopy; 2.1 Skill Acquisition; 2.2 Enjoying Knowledge; 2.3 Budget-Friendly; 2.4 Low Training Requirement; 2.5 Robust; 2.6 Reliable; 2.7 Easy Troubleshooting; 2.8 The Technology Doesn't Age; 2.9 Easy to Move; References; 3: Buying a Microscope; 3.1 Decide What Is Needed 3.1.1 Who Is Going to Be Using the Microscope?3.1.2 What Type of Samples Are the Users Working with?; 3.1.3 How Often Is the Microscope Going to Be Used?; 3.1.4 Where Is the Microscope Going to Be Used?; 3.1.5 Will It Stay Set Up or Will It Need to Be Packed Up After Each Use?; 3.1.6 Does the User Require Image Capture?; 3.2 Budget and Where to Invest; 3.3 Microscopes for Children; 3.4 Buying a Second-Hand Microscope; 4: The Science of Light; 4.1 What Is Light?; 4.2 The Basics of Light; 4.3 Colours of Light; 4.4 Movement of Light; 4.4.1 Refraction; 4.4.2 Reflection 4.4.3 Absorption and Emission4.4.4 Scatter; 4.5 Photosensitivity: How We Perceive Light; 4.5.1 The Evolution of Vision; 4.5.2 Photosensitive Cells; 4.5.3 Human Vision; 4.5.4 How We See; 4.5.5 Colour Vision; 4.5.6 Variation of Vision Between Individuals; 4.5.7 Colour-Blindness; 4.5.8 Variation in Physiology; References; 5: Introduction to Lenses; 5.1 Lens Shape and Function; 5.2 Convex Lenses; 5.3 Concave Lens; 5.4 Lens Coating; 5.5 Anti-reflective Coating; 5.6 Prisms; 5.7 Magnification; 5.8 Numerical Aperture; 5.9 Resolution; 5.10 Focus; 5.11 Aberrations; References 6: The Anatomy of the Microscope6.1 Styles of Microscope; 6.2 The Upright Microscope; 6.3 The Inverted Microscope; 6.4 The Stereomicroscope; 6.4.1 Types of Stereomicroscope; 6.5 Petrographic Microscope; 7: The Eyepiece; 7.1 Compensating Eyepieces; 7.2 Eyepoint; 7.3 Interpupillary Distance; 7.4 Using Eyepieces as a Glasses Wearer; 7.5 Diopter Eyepieces; 7.5.1 How to Adjust a Diopter Eyepiece; 7.6 Reticle; 7.6.1 How to Calibrate a Reticle; 7.7 Cleaning an Eyepiece; 7.8 Scale Bars on Digital Systems; References; 8: The Objective; 8.1 How to Read an Objective 8.1.1 Achromatic and Apochromatic8.1.2 Infinity Correction; 8.1.3 Magnification; 8.1.4 Numerical Aperture (NA); 8.1.5 Planar; 8.1.6 Refractive Index (RI); 8.1.7 Thread Depth and Brand Compatibility; 8.1.8 Working Distance (WD); 8.2 Air Objective; 8.3 Oil Immersion Objective; 8.3.1 How to Use an Air Objective; 8.3.2 How to Use an Oil Immersion Objective; 8.4 Cleaning an Objective; 8.4.1 Cleaning an Air Objective; 8.4.2 Cleaning an Oil Objective; 8.5 Summary of Objective Codes; Reference; 9: The Stage; 9.1 Focusing the Sample; 9.2 Slide Navigation Controls
Responsibility: Dee Lawlor.

Abstract:

This book offers a beginner's guide to using light microscopes. This book is intended for anyone using a light microscope, such as university students, people in lab environments, hobbyists,  Read more...

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