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An introduction to single molecule biophysics

Author: Yuri L Lyubchenko
Publisher: Boca Raton : CRC Press, Taylor & Francis Group, [2018]
Series: Foundations of biochemistry and biophysics.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
"This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and  Read more...
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Genre/Form: Electronic books
Electronic book
Additional Physical Format: Print version:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Yuri L Lyubchenko
ISBN: 1351333127 9781351333122 9781351333092 1351333097 9780203702338 0203702336 9781439806951 1439806950
OCLC Number: 1024173975
Description: 1 online resource (xv, 234 pages) : color illustrations.
Contents: Single molecule fluorescence / Ashok A. Deniz --
Atomic force microscope for topographic studies / Yuri L. Lyubchenko --
AFM force spectroscopy / Eric A. Josephs, Zackary N. Scholl, and Piotr E. Marszalek --
Magnetic tweezers / Yuri L. Lyubchenko, Zhiqiang Sun, and Piero R. Bianco --
Optical tweezers / Marco Capitanio --
Nanofluidic transport and sensing in biological and artificial nanopores / Aleksandr Noy and Meni Wanunu.
Series Title: Foundations of biochemistry and biophysics.
Responsibility: edited by Yuri L. Lyubchenko, College of Pharmacy, Unversity of Nebraska Medical Center.

Abstract:

"This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications. Covers the most widely used single molecule biophysics techniquesProvides numerous examples of current biological applicationsIncludes practical tips on measurement optimization, resolution limits, etc. Yuri L. Lyubchenko is Professor of Pharmaceutical Sciences at the University of Nebraska Medical Center."--Provided by publisher.

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