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IVF Techniques for the Beginners

Author: Talwar Pankaj Jain Kuldeep; Jaypee Brothers (Jaypeedigital)
Publisher: Jaypee Brothers Medical Publisher (P) Ltd., 2013.
Edition/Format:   Print book : English : 1/e
Publication:Jaypee eBooks.
Summary:
This book, IVF Techniques for the Beginners, deals with in vitro fertilization (IVF) techniques. In the past few decades, there has been continuous research in the field of in vitro fertilization and related technology. There has been immense growth in understanding the human gamete behavior and fertilization process leading to increase in the pregnancy rates following IVF. Newer and better infrastructure and  Read more...
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Additional Physical Format: Printed edition:
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Talwar Pankaj Jain Kuldeep; Jaypee Brothers (Jaypeedigital)
ISBN: 9789350903599 9350903598
OCLC Number: 1097203644
Description: 316 : digital
Contents: Section 1 IVF Simplified --
1 Ovum Pick-up / S Shamila Shamila, S 3 --
Egg retrieval process 3 --
What happens in the Lab After the Eggs are Aspirated? 8 --
2 Embryo Transfer / Gautam N Allahbadia Allahbadia, Gautam N, Rubina Merchant Merchant, Rubina, Gorat Gandhi Gandhi, Gorat 12 --
Clinical Discussion 14 --
Recent Advances 30 --
3 Media and Quality Control in IVF Laboratory / Jayant G Mehta Mehta, Jayant G 36 --
Quality Control 37 --
Quality Assurance 39 --
Quality Improvement (Quality Audit) 40 --
Internal Quality Control 40 --
Quality Management System 40 --
ART Laboratory 40 --
4 Sperm Preparation for in Vitro Fertilization and Intracytoplasmic Sperm Injection / Kuldeep Jain Jain, Kuldeep 65 --
Method 66 --
5 Sperm Retrieval Techniques (PESA, TESA, MESA, MICRO-TESE, TESE) / RK Sharma Sharma, RK 72 --
Etiopathogenesis of Azoospermia 72 --
Diagnosis 73 --
Management 74 --
6 Surgically Retrieved Sperm Samples for ICSI / Ethiraj Balaji Prasath Prasath, Ethiraj Balaji 81 --
Background 81 --
Retrieval Procedures 81 --
Patients indicated for MESA/TESE/PESA/TESA 82 --
Surgical Retrieval of Sperm 83 --
Cryopreservation of Surgically Retrieved Sperm 84 --
Sperm Preparation on the Day of ICSI 87 --
Safety of Surgically Retrieved Sperm 88 --
7 Intracytoplasmic Morphologically Selected Sperm Injection / Rajvi H Mehta Mehta, Rajvi H 90 --
Selection of Sperms-Natural Conception and Art 91 --
Sperm Morphology and Pregnancy Outcome 91 --
Vacuoles-DNA Fragmentation and Abnormal Chromatin Packaging 92 --
Abnormal DNA Packaging and its Impact on Fertilization, Embryonic Development and Pregnancy 93 --
Selection of sperms for ICSI 94 --
Steps for IMSI 95 --
Preparation of IMSI Dish 95 --
8 Vitrification / Gaurav Majumdar Majumdar, Gaurav 103 --
Principle of Vitrification 104 --
Various Factors Determine Ultimate Survival 105 --
Cryoprotectants 106 --
Vitrification Carrier Devices 107 --
Vitrification-Closed Systems 110 --
Elements of a Typical Vitrification Procedure 111 --
9 Ovarian Cortex Freezing / Pankaj Talwar Talwar, Pankaj 114 --
Introduction 114 --
Indications for Ovarian Cryopreservation 117 --
Assessment of Ovarian Reserve 119 --
Current Techniques of Fertility Preservation 120 --
Ovarian cortex Cryopreservation 121 --
Limitations for Ovarian Tissue Cryopreservation 122 --
Collection of Ovarian Tissue 123 --
Cryoprotectanl Preparation 123 --
Tissue Preparation 126 --
Histological Analysis 130 --
Steps of Ovarian Cortex Freezing 130 --
Peculiarities of Ovarian Tissue Freezing 137 --
Methods of Thawing 137 --
Transplant 139 --
Ethical Concerns in Ovarian Tissue Cryopreservation 139 --
10 Preimplantation Genetic Diagnosis: An Emergent Technology / Shailaja Gada Saxena Saxena, Shailaja Gada, Prabhat Ranjan Ranjan, Prabhat 143 --
Indications for Preimplantation Genetic Diagnosis 144 --
Preimplantation Genetic Diagnosis-Historical Perspective 144 --
Steps Involved in Preimplantation Genetic Diagnosis 145 --
Evolution of PGD for Aneuploidies, Structural Chromosomal Rearrangements and Preimplantation Genetic Screening 150 --
Evolution of Preimplantation Genetic Diagnosis for Monogenic Disorders 152 --
Preimplantation Genetic Diagnosis for Human Leukocyte Antigen Matching: Saviour Sibling 153 --
Preimplantation Genetic Diagnosis for Cancer Predisposition 154 --
Preimplantation Genetic Diagnosis-Current Global Status 154 --
Preimplantation Genetic Diagnosis-An Indian Perspective 156 --
Challenges Faced with Preimplantation Genetic Diagnosis 157 --
Ethical Legal and Social Issues 158 --
Conclusion 159 --
11 Assisted Hatching / Natachandra Chimote Chimote, Natachandra, Nishad Chimote Chimote, Nishad 166 --
Background 166 --
General Principles for AH 168 --
Excessive Fragmentation 169 --
Poor Embryo Growth Rate/Morphology 172 --
Assisted Hatching using Acidic Tyrode's Solution 174 --
12 Overview of Cryobiology in Assisted Reproductive Technology / Pankaj Talwar Talwar, Pankaj 182 --
Basic Definitions 183 --
Thermodynamics 184 --
Physics 184 --
Concept of Latent Heat 185 --
Biology of Cryofreezing 186 --
Cryoprotectants 187 --
Principles of Cryobiology 188 --
Steps of Cryopreservation 189 --
Events During Freezing 193 --
Freeze Injuries 193 --
Events During Thawing 194 --
Thaw Injuries 194 --
13 Simplifying IVF Program: Satellite Module / Kuldeep Jain Jain, Kuldeep 197 --
14 Fertility Preservation in Female Cancer Patients / Pankaj Talwar Talwar, Pankaj 199 --
Indications of Offering Fertility Preservation 200 --
Vulnerability of the Reproductive System to Cancer Treatment 201 --
Mode of Action of Gonadotoxic Agents 201 --
Assessment of Ovarian Reserve 203 --
Fertility Preservation Options 203 --
Ethical Concerns in Ovarian Tissue Cryopreservation 213 --
Novel Options of Fertility Preservation 213 --
15 ICSI-An Introduction / Ethiraj Balaji Prasath Prasath, Ethiraj Balaji 218 --
Instrumentation for ICSI 219 --
Microtools 222 --
Preparation of Culture Dishes 223 --
Set-up of Dishes 224 --
Sperm Preparation 225 --
Denuding of Oocytes for ICSI 226 --
Setting-up Microscope 228 --
Narishige 228 --
Research Instruments 229 --
Eppendorf 229 --
Sperm Injection 230 --
Special Circumstances of Applying ICSI 231 --
Rescue ICSI 233 --
Fertilization in ICSI 233 --
Personal Care of ICSI Practitioner 234 --
16 Guidelines and Legislation on Assisted Reproduction Technology in India / RS Sharma Sharma, RS 236 --
Section 2 Essential Protocols in ART --
1 Sperm Freezing / Pankaj Talwar Talwar, Pankaj, Kuldeep Jain Jain, Kuldeep 255 --
2 Embryo Transfer / Sonia Malik Malik, Sonia 259 --
Disposables and Equipment Required 259 --
Reagents 259 --
Embryo Transfer-Procedure 259 --
3 Intracytoplasmic Sperm Injection Protocols / Rishma D Pai Pai, Rishma D, Nandita P Palshetkar Palshetkar, Nandita P, Hrishikesh D Pai Pai, Hrishikesh D 263 --
Intracytoplasmic Sperm Injection Media and Pipettes 263 --
Preparation of Intracytoplasmic Sperm Injection Dishes 264 --
Denudation 265 --
Manipulation of Spermatozoa 266 --
Manipulation of Oocytes 267 --
Microinjection of Oocytes with Mature Spermatozoa 269 --
4 Oocyte Cryopreservation / Jaidcep Malhotra Malhotra, Jaidcep, Narendra Malhotra Malhotra, Narendra 271 --
Oocyte Cryopreservation-Slow Cooling 271 --
Oocyte Cryopreservation-Vitrification 274 --
Oocyte warming protocol 277 --
5 Testicular Biopsy Banking / Jaideep Malhotra Malhotra, Jaideep, Narendra Malhotra Malhotra, Narendra, Pankaj Talwar Talwar, Pankaj 278 --
Technique for Cryopreservation of Testicular Sperm 278 --
6 Ovum Pick-up / Sohani Verma Verma, Sohani 281 --
Equipment Required 281 --
Reagents Required 281 --
Procedure: OPU Step-by-Step 281 --
7 Follicular Trekking and Use of Color Doppler in ART Cycles / Bharti Jain Jain, Bharti 284 --
Equipment Required 284.
Responsibility: Jain Kuldeep, Talwar Pankaj.

Abstract:

IVF Techniques for Beginners brings clinicians fully up to date with the latest advances in equipment and techniques for In-Vitro Fertilisation (IVF).  Read more...

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