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Label-free and multi-parametric monitoring of cell-based assays with substrate-embedded sensors

Author: Maximilian Oberleitner
Publisher: Cham : Springer, [2018]
Series: Springer theses.
Edition/Format:   eBook : Document : English : 1st ed. 2017View all editions and formats
Summary:
This thesis describes novel substrate embedded physical sensors that can be used to monitor different types of cell-based assays non-invasively and label-free. The sensors described provide integrative information of the cells under study with an adaptable time resolution (ranging from milliseconds to days). This information about the dynamic cell response to chemical, physical or biological stimuli defines a new  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Printed edition:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Maximilian Oberleitner
ISBN: 9783319453842 331945384X 3319832794 9783319832791
OCLC Number: 1005200910
Description: 1 online resource
Contents: Introduction --
Objectives --
Methods & Instrumentation --
MQCM: Multiple Cytomechanic Sensing --
QCM-ECIS: Combined Viscoelastic and Dielectric Sensing of Cells --
QCM-OCS: Optochemical Sensing of Temperature and pO2 in the Cell Surface Junction --
Summary --
Appendix.
Series Title: Springer theses.
Responsibility: Maximilian Oberleitner.

Abstract:

This thesis describes novel substrate embedded physical sensors that can be used to monitor different types of cell-based assays non-invasively and label-free. The sensors described provide integrative information of the cells under study with an adaptable time resolution (ranging from milliseconds to days). This information about the dynamic cell response to chemical, physical or biological stimuli defines a new paradigm in fundamental biomedical research. The author, Maximilian Oberleitner, describes approaches in which the cells are directly grown on different sensor surfaces (gold-film electrodes, shear wave resonators or dye-doped polymer films). This approach, with the reacting cells in particularly close proximity and contact with the sensor surface, is key to a remarkable sensitivity, opening the way for a variety of new applications. This thesis not only introduces the fundamentals of each approach, but it also describes in great detail the design principles and elucidates the boundary conditions of the new sensors.

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