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A laboratory course in tissue engineering.

Author: Melissa Kurtis Micou; Dawn M Kilkenny
Publisher: Boca Raton : CRC Press, 2013.
Edition/Format:   eBook : Document : EnglishView all editions and formats
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Genre/Form: Electronic books
Laboratory manuals
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Melissa Kurtis Micou; Dawn M Kilkenny
ISBN: 9781466575233 1466575239
OCLC Number: 908077866
Description: 1 online resource (287 pages)
Contents: Getting Started in the LabLab SafetyEssential Lab Skills for Tissue EngineersIsolation of Primary Chondrocytes from Bovine Articular CartilageMeasuring and Modeling Growth of a Cell PopulationPurification of a Cell Population Using Magnetic Cell SortingDecellularized Matrices for Tissue EngineeringEffect of Plating Density on Cell Adhesion to Varied Culture MatricesDynamic versus Static Seeding of Cells onto Biomaterial ScaffoldsCell Patterning Using Microcontact PrintingMeasuring and Modeling the Motility of a Cell Population Using an Under-Agarose AssayCharacterizing Matrix Remodeling through Collagen Gel ContractionEffect of Substrate Stiffness on Cell DifferentiationEffect of Culture Configuration (Two versus Three Dimensions) on Matrix AccumulationCombining In Silico and In Vitro Techniques to Engineer Pluripotent Stem Cell FateThe FahraeusLindqvist Effect: Using Microchannels to Observe Small Vessel HemodynamicsExamining Single-Cell Mechanics Using a Microfluidic Micropipette Aspiration SystemContribution of Tissue Composition to Bone Material PropertiesTechnical Communication: Presenting Your FindingsAppendix 1: Trypsinizing a Cell MonolayerAppendix 2: Counting Cells with a HemacytometerAppendix 3: PicoGreen (R) DNA AssayAppendix 4: Dimethylmethylene Blue (DMMB) Assay for Sulfated GlycosaminoglycansAppendix 5: Microfluidic Device Design and FabricationIndex

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"The book is well organized to teach cell culture and tissue engineering experiments to novice and experienced students. There is a quantitative emphasis in the book that strengthens the Read more...

 
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