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Lipid-activated nuclear receptors : methods and protocols

Author: Matthew C Gage; Inés Pineda-Torra
Publisher: New York, N.Y. : Humana Press : Springer, [2019] ©2019
Series: Methods in molecular biology (Clifton, N.J.), v. 1951.
Edition/Format:   eBook : Document : EnglishView all editions and formats

This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in  Read more...


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Genre/Form: Electronic books
Laboratory Manual
Laboratory manuals
Additional Physical Format: Print version:
Lipid-activated nuclear receptors.
New York, N.Y : Springer, ©2019
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Matthew C Gage; Inés Pineda-Torra
ISBN: 9781493991303 1493991302
OCLC Number: 1089150466
Description: 1 online resource (xi, 220 pages) : illustrations (some color)
Contents: Quantification of oxysterol nuclear receptor ligands by LC/MS/MS / Lilia Magomedova and Carolyn L. Cummins --
Stable luciferase reporter system to characterize LXR regulation by oxysterols and novel ligands / Samantha A. Hutchinson and James L. Thorne --
Isolation and analysis of human monocytes and adipose tissue macrophages / Jean-Baptiste Julla, Raphaelle Ballaire, Tina Ejlalmanesh, Jean-Francois Gautier, Nicolas Venteclef, and Fawaz Alzaid --
Glucan-encapsulated siRNA particles (GeRPs) for specific gene silencing in adipose tissue macrophages / Emelie Barreby, Andre´ Sulen, and Myriam Aouadi --
Isolation and purification of tissue resident macrophages for the analysis of nuclear receptor activity / Laura Alonso-Herranz, Jesus Porcuna, and Mercedes Ricote --
Bone marrow-derived macrophage immortalization of LXR nuclear receptor-deficient cells / Ana Ramon-Vazquez, Juan Vladimir de la Rosa, Carlos Tabraue, and Antonio Castrillo --
Dual cross-linking chromatin immunoprecipitation protocol for next-generation sequencing (ChIPseq) in macrophages / David A. Rollins and Inez Rogatsky --
Analysis of LXR nuclear receptor cistrome through ChIP-seq data bioinformatics / Juan Vladimir de la Rosa, Ana Ramon-Vazquez, Carlos Tabraue, and Antonio Castrillo --
Quantifying cellular cholesterol efflux / Sabrina Robichaud and Mireille Ouimet --
Methods for assessing the effects of LXR agonists on macrophage bacterial infection / Estibaliz Glarıa, Jonathan Matalonga, and Annabel F. Valledor --
Measuring apoptotic cell engulfment (efferocytosis) efficiency / Matthew C. Gage --
Methods to study monocyte and macrophage trafficking in atherosclerosis progression and resolution / Ada Weinstock and Edward A. Fisher --
Preparation of frozen liver tissues for integrated omics analysis / Ning Liang, Rongrong Fan, Saioa Gon˜i, and Eckardt Treuter --
Identification of nuclear receptor targets by chromatin immunoprecipitation in fatty liver / Natalia Becares and Ines Pineda-Torra --
LPS/D-galactosamine-induced fulminant hepatitis model to assess the role of ligand-activated nuclear receptors on the NLRP3 inflammasome pathway in vivo / Yasmine Sebti, Lise Ferri, Mathilde Zecchin, Justine Beauchamp, Denis Mogilenko, Bart Staels, Helene Duez, and Benoit Pourcet --
Analyzing T-cell plasma membrane lipids by flow cytometry / Kirsty E. Waddington, Ines Pineda-Torra, and Elizabeth C. Jury.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1951.
Responsibility: edited by Matthew C. Gage and Inés Pineda-Torra.


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