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Lipid signaling protocols

Author: Banafshé Larijani; Rudiger Woscholski; Colin A Rosser
Publisher: New York, N.Y. : Humana ; [London] : [Springer, distributor], ©2009.
Series: Methods in molecular biology (Clifton, N.J.), v. 462.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:
'Lipid Signaling Protocols' assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function.
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Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Banafshé Larijani; Rudiger Woscholski; Colin A Rosser
ISBN: 9781588297273 1588297276 9781603271158 1603271155
OCLC Number: 166358111
Description: xx, 429 pages [8] pages of plates : illustrations (some color) ; 25 cm.
Contents: Eicosanoids : generation and detection in mammalian cells / Valerie B. O'Donnell, Ben Maskrey, and Graham W. Taylor --
Lipidomic analysis of phospholipids and related structures by liquid chromatography-mass spectrometry / Trevor R. Pettitt --
Measurement of polyphosphoinositides in cultured mammalian cells / Frank T. Cooke --
Inositol lipid-dependent functions in Saccharomyces cerevisiae : analysis of phosphatidylinositol phosphates / Stephen K. Dove and Robert H. Michell --
Methods for the determination of the masss of nuclear PtdIns4P, PtdIns5P, and PtdIns(4,5)P<<SUBSCRIPT 2>> / David R. Jones [and others] --
Lipid quantification and structure determination of nuclear envelope precursor membranes in the sea urchin / Marie Garnier-Lhomme [and others] --
Solution and solid-state NMR of lipids / Axelle Grelard [and others] --
Spatial localization of PtdInsP<<SUBSCRIPT 2>> in phase-separated giant unilamellar vesicles with a fluorescent PLC-delta 1 PH domain / Xavier Mulet [and others] --
Studying cell-to-cell interactions : an easy method of tethering ligands on artificial membranes / Sebastian J. Fleire and Facundo D. Batista --
Ceramide-induced transbilayer (flip-flop) lipid movement in membranes / F.-Xabier Contreras [and others] --
Effects of sterols on the development and aging of Caenorhabditis elegans / Eun-Young Lee [and others] --
Proteomic analysis of the sterol-mediated signaling pathway in Caenorhabditis elegans / Byung-Kwon Choi [and others] --
Sudying the subcellular localization and DNA-binding activity of FoxO transcription factors, downstream effectors of P13K/Akt / Abdelkader Essafi [and others] --
Measurement of PTEN activity in vivo by imaging phosphorylated Akt / Erika Rosivatz and Rudiger Woscholski --
Analysis of phosphatidylinositol 3,4,5 trisphosphate 5-phosphatase activity by in vitro and in vivo assays / Lisa M. Ooms [and others] --
Measuring phospholipase D activity in insulin-secreting pancreatic [beta]-cells and insulin-responsive muscle cells and adipocytes / Rosanna Cazzolli [and others] --
Protein kinase C as an effector of lipid-derived second messengers / Marie-Hélène Paclet [and others] --
Detection of myotubularin phosphatases activity on phosphoinositides in vitro and ex vivo / Holger Maria Rohde [and others] --
Quantification of multiple phosphatidylinositol 4-kinase isozyme activities in cell extracts / Mark G. Waugh, Shane Minogue, and J. Justin Hsuan --
Phospholipid-interacting proteins by solution-state NMR spectroscopy / Keiichiro Kami, Sundaresan Rajesh, and Michael Overduin --
Revealing signaling in single cells by single- and two-photon fluorescence lifetime imaging microscopy / Damien Alcor, Veronique Calleja, and Banafshé Larijani --
Phosphoinositide (PI) 3-kinase assays / Michael J. Fry --
Measurement of phosphatidylinositol and phosphatidylcholine binding and transfer activity of the lipid transport Protein PITP / Shamshad Cockcroft --
In vitro reconstitution of activation of PLC[epsilon] by Ras and Rho GTPases / Natalia Lamuño Gandarillas [and others] --
Assaying endogenous phosphatidylinositol-4-phosphate 5-kinase (PIP5K) activities / Jonathon R. Halstead [and others] --
Preparation of membrane rafts / Mark G. Waugh and J. Justin Hsuan.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 462.
Responsibility: Banafshé Larijani, editor ; Rudiger Woscholski, editor ; Colin A. Rosser, editor.

Abstract:

In a single volume this book assembles the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. This is an essential resource  Read more...

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