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Luminescence de complexes de lanthanide par effet d'antenne à deux photons : vers l'imagerie fonctionnelle

Author: Ngoc Anh Thy BuiOlivier MauryBruno AndriolettiLoïc CharbonnièreLudovic JullienAll authors
Publisher: 2016.
Dissertation: Thèse de doctorat : Chimie : Lyon : 2016.
Edition/Format:   Computer file : Document : Thesis/dissertation : French
Summary:
Les ions lanthanides possèdent des propriétés photophysiques particulières, qui ont suscité une forte attention dans le développement de sondes biologiques luminescentes. En effet, les avantages que présentent leurs émissions en raies fines et caractéristiques, ainsi que leurs temps de vie de luminescence longs, permettent diverses applications en imagerie. Cependant, le faible coefficient d'absorption
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Details

Genre/Form: Thèses et écrits académiques
Material Type: Document, Thesis/dissertation
Document Type: Computer File
All Authors / Contributors: Ngoc Anh Thy Bui; Olivier Maury; Bruno Andrioletti; Loïc Charbonnière; Ludovic Jullien; Chantal Andraud; Université de Lyon (2015-....).; École Doctorale de Chimie (Lyon).; École normale supérieure de Lyon.; Laboratoire de Chimie (UMR 5182).
OCLC Number: 960197142
Notes: Titre provenant de l'écran-titre.
Description: 1 online resource
Responsibility: Ngoc Anh Thy Bui ; sous la direction de Olivier Maury.

Abstract:

Les ions lanthanides possèdent des propriétés photophysiques particulières, qui ont suscité une forte attention dans le développement de sondes biologiques luminescentes. En effet, les avantages que présentent leurs émissions en raies fines et caractéristiques, ainsi que leurs temps de vie de luminescence longs, permettent diverses applications en imagerie. Cependant, le faible coefficient d'absorption molaire de ces éléments conduit à préférer une excitation par le biais d'une antenne capable de transférer l'énergie au métal.Par ailleurs, l'utilisation de l'absorption biphotonique a montré de nombreux avantages, puisqu'elle permet une excitation de la molécule à une longueur d'onde deux fois plus grande que l'excitation à un photon : cette longueur d'onde peut alors se situer dans la fenêtre de transparence biologique (650 - 1100 nm).Les travaux de cette thèse visent à combiner les avantages des lanthanides avec ceux de l'excitation biphotonique. Les complexes synthétisés sont basés sur une plateforme triazacyclononane substituée par des antennes à deux-photons. Leurs propriétés spectroscopiques à un photon ont été étudiées, et ont alors permis de réaliser des images cellulaires en multiplexing à deux photons utilisant Eu3+ et Tb3+. Des sondes biphotoniques de brillance optimisée ont également été obtenues pour Sm3+, Tb3+ et Dy3+, et appliquées à l'imagerie. L'étude approfondie d'un des complexes de terbium(III) a de plus mis en évidence sa sensibilité à la viscosité. L'analyse des propriétés spectroscopiques de cette sonde a permis d'en rationaliser le comportement photophysique, ouvrant ainsi la voie à des applications originales en imagerie fonctionnelle. Enfin, nous avons montré une internalisation rapide de complexes de lanthanide cationiques de type cyclen ou cyclam par des cellules vivantes, et l'étude de ces nouvelles structures offre de nouvelles perspectives dans le développement de bio-sondes de lanthanides.

Lanthanide ions demonstrate peculiar photophysical properties that has attracted a substantial attention in the development of luminescent bioprobes. Indeed, the advantages stemming from their sharp and characteristic emissions, as well as their long luminescence lifetimes, enable various imaging applications. However, the weak molar extinction coefficient of these elements results in a preferential excitation through an antenna, which transfers its energy to the metal.On the other hand, biphotonic absorption showed numerous advantages, since excitation of a molecule can be achieved at a wavelength twice as high as using one-photon excitation. This wavelength may therefore reach the optical transparency window (650 - 1100 nm).The work carried out during this PhD thesis aims at combining the advantages of lanthanides with those of biphotonic excitation. The synthesized complexes are based on a triazacyclononane platform substituted with two-photon antennae. Their one-photon spectroscopic properties have been studied, and enabled to perform two-photon multiplexed cellular imaging, using Eu3+ and Tb3+. Biphotonic probes with an optimized brightness have been obtained for Sm3+, Tb3+ and Dy3+, and applied to cell imaging. The thorough study of one of the terbium(III) complexes has moreover displayed evidence of a sensitivity towards viscosity. Analyzing the spectroscopic properties of this probe has therefore allowed to rationalize its photophysical behavior, paving the way for original functional imaging applications. Lastly, a rapid internalization of cationic lanthanide complexes with a cyclen or cyclam framework by living cells has been proved. Thus, the study of these new structures presents new perspectives for the development of lanthanide bioprobes.

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\n\n

Primary Entity<\/h3>\n
<http:\/\/www.worldcat.org\/oclc\/960197142<\/a>> # Luminescence de complexes de lanthanide par effet d\'antenne \u00E0 deux photons : vers l\'imagerie fonctionnelle<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nbgn:ComputerFile<\/a>, bgn:Thesis<\/a>, schema:MediaObject<\/a>, schema:CreativeWork<\/a> ;\u00A0\u00A0\u00A0\nbgn:inSupportOf<\/a> \"Th\u00E8se de doctorat : Chimie : Lyon : 2016.<\/span>\" ;\u00A0\u00A0\u00A0\nlibrary:oclcnum<\/a> \"960197142<\/span>\" ;\u00A0\u00A0\u00A0\nlibrary:placeOfPublication<\/a> <http:\/\/id.loc.gov\/vocabulary\/countries\/fr<\/a>> ;\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Thing\/absorption_biphotonique<\/a>> ; # Absorption biphotonique<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Thing\/lanthanides<\/a>> ; # Lanthanides<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Topic\/viscosite<\/a>> ; # Viscosit\u00E9<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Topic\/microscopie<\/a>> ; # Microscopie<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Topic\/imagerie_optique_dissertation_universitaire<\/a>> ; # Imagerie optique--Dissertation universitaire<\/span>\n\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Topic\/complexes_de_coordination_dissertation_universitaire<\/a>> ; # Complexes de coordination--Dissertation universitaire<\/span>\n\u00A0\u00A0\u00A0\nschema:author<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Person\/bui_ngoc_anh_thy_1989<\/a>> ; # Ngoc Anh Thy Bui<\/span>\n\u00A0\u00A0\u00A0\nschema:contributor<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Organization\/ecole_normale_superieure_de_lyon<\/a>> ; # \u00C9cole normale sup\u00E9rieure de Lyon.<\/span>\n\u00A0\u00A0\u00A0\nschema:contributor<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Person\/jullien_ludovic_1963<\/a>> ; # Ludovic Jullien<\/span>\n\u00A0\u00A0\u00A0\nschema:contributor<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Person\/maury_olivier<\/a>> ; # Olivier Maury<\/span>\n\u00A0\u00A0\u00A0\nschema:contributor<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Organization\/ecole_doctorale_de_chimie_lyon<\/a>> ; # \u00C9cole Doctorale de Chimie (Lyon).<\/span>\n\u00A0\u00A0\u00A0\nschema:contributor<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Organization\/laboratoire_de_chimie_umr_5182<\/a>> ; # Laboratoire de Chimie (UMR 5182).<\/span>\n\u00A0\u00A0\u00A0\nschema:contributor<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Person\/andrioletti_bruno_1968<\/a>> ; # Bruno Andrioletti<\/span>\n\u00A0\u00A0\u00A0\nschema:contributor<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Organization\/universite_de_lyon_2015<\/a>> ; # Universit\u00E9 de Lyon (2015-....).<\/span>\n\u00A0\u00A0\u00A0\nschema:contributor<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Person\/charbonniere_loic<\/a>> ; # Lo\u00EFc Charbonni\u00E8re<\/span>\n\u00A0\u00A0\u00A0\nschema:contributor<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Person\/andraud_chantal_1956<\/a>> ; # Chantal Andraud<\/span>\n\u00A0\u00A0\u00A0\nschema:datePublished<\/a> \"2016<\/span>\" ;\u00A0\u00A0\u00A0\nschema:description<\/a> \"Lanthanide ions demonstrate peculiar photophysical properties that has attracted a substantial attention in the development of luminescent bioprobes. Indeed, the advantages stemming from their sharp and characteristic emissions, as well as their long luminescence lifetimes, enable various imaging applications. However, the weak molar extinction coefficient of these elements results in a preferential excitation through an antenna, which transfers its energy to the metal.On the other hand, biphotonic absorption showed numerous advantages, since excitation of a molecule can be achieved at a wavelength twice as high as using one-photon excitation. This wavelength may therefore reach the optical transparency window (650 - 1100 nm).The work carried out during this PhD thesis aims at combining the advantages of lanthanides with those of biphotonic excitation. The synthesized complexes are based on a triazacyclononane platform substituted with two-photon antennae. Their one-photon spectroscopic properties have been studied, and enabled to perform two-photon multiplexed cellular imaging, using Eu3+ and Tb3+. Biphotonic probes with an optimized brightness have been obtained for Sm3+, Tb3+ and Dy3+, and applied to cell imaging. The thorough study of one of the terbium(III) complexes has moreover displayed evidence of a sensitivity towards viscosity. Analyzing the spectroscopic properties of this probe has therefore allowed to rationalize its photophysical behavior, paving the way for original functional imaging applications. Lastly, a rapid internalization of cationic lanthanide complexes with a cyclen or cyclam framework by living cells has been proved. Thus, the study of these new structures presents new perspectives for the development of lanthanide bioprobes.<\/span>\"@fr<\/a> ;\u00A0\u00A0\u00A0\nschema:description<\/a> \"Les ions lanthanides poss\u00E8dent des propri\u00E9t\u00E9s photophysiques particuli\u00E8res, qui ont suscit\u00E9 une forte attention dans le d\u00E9veloppement de sondes biologiques luminescentes. En effet, les avantages que pr\u00E9sentent leurs \u00E9missions en raies fines et caract\u00E9ristiques, ainsi que leurs temps de vie de luminescence longs, permettent diverses applications en imagerie. Cependant, le faible coefficient d\'absorption molaire de ces \u00E9l\u00E9ments conduit \u00E0 pr\u00E9f\u00E9rer une excitation par le biais d\'une antenne capable de transf\u00E9rer l\'\u00E9nergie au m\u00E9tal.Par ailleurs, l\'utilisation de l\'absorption biphotonique a montr\u00E9 de nombreux avantages, puisqu\'elle permet une excitation de la mol\u00E9cule \u00E0 une longueur d\'onde deux fois plus grande que l\'excitation \u00E0 un photon : cette longueur d\'onde peut alors se situer dans la fen\u00EAtre de transparence biologique (650 - 1100 nm).Les travaux de cette th\u00E8se visent \u00E0 combiner les avantages des lanthanides avec ceux de l\'excitation biphotonique. Les complexes synth\u00E9tis\u00E9s sont bas\u00E9s sur une plateforme triazacyclononane substitu\u00E9e par des antennes \u00E0 deux-photons. Leurs propri\u00E9t\u00E9s spectroscopiques \u00E0 un photon ont \u00E9t\u00E9 \u00E9tudi\u00E9es, et ont alors permis de r\u00E9aliser des images cellulaires en multiplexing \u00E0 deux photons utilisant Eu3+ et Tb3+. Des sondes biphotoniques de brillance optimis\u00E9e ont \u00E9galement \u00E9t\u00E9 obtenues pour Sm3+, Tb3+ et Dy3+, et appliqu\u00E9es \u00E0 l\'imagerie. L\'\u00E9tude approfondie d\'un des complexes de terbium(III) a de plus mis en \u00E9vidence sa sensibilit\u00E9 \u00E0 la viscosit\u00E9. L\'analyse des propri\u00E9t\u00E9s spectroscopiques de cette sonde a permis d\'en rationaliser le comportement photophysique, ouvrant ainsi la voie \u00E0 des applications originales en imagerie fonctionnelle. Enfin, nous avons montr\u00E9 une internalisation rapide de complexes de lanthanide cationiques de type cyclen ou cyclam par des cellules vivantes, et l\'\u00E9tude de ces nouvelles structures offre de nouvelles perspectives dans le d\u00E9veloppement de bio-sondes de lanthanides.<\/span>\"@fr<\/a> ;\u00A0\u00A0\u00A0\nschema:exampleOfWork<\/a> <http:\/\/worldcat.org\/entity\/work\/id\/3900681303<\/a>> ;\u00A0\u00A0\u00A0\nschema:genre<\/a> \"Th\u00E8ses et \u00E9crits acad\u00E9miques<\/span>\"@fr<\/a> ;\u00A0\u00A0\u00A0\nschema:inLanguage<\/a> \"fr<\/span>\" ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Luminescence de complexes de lanthanide par effet d\'antenne \u00E0 deux photons : vers l\'imagerie fonctionnelle<\/span>\"@fr<\/a> ;\u00A0\u00A0\u00A0\nschema:productID<\/a> \"960197142<\/span>\" ;\u00A0\u00A0\u00A0\nwdrs:describedby<\/a> <http:\/\/www.worldcat.org\/title\/-\/oclc\/960197142<\/a>> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n\n

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<http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Thing\/lanthanides<\/a>> # Lanthanides<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Thing<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Lanthanides<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
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<http:\/\/experiment.worldcat.org\/entity\/work\/data\/3900681303#Topic\/viscosite<\/a>> # Viscosit\u00E9<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Viscosit\u00E9<\/span>\"@fr<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/id.loc.gov\/vocabulary\/countries\/fr<\/a>>\u00A0\u00A0\u00A0\u00A0a \nschema:Place<\/a> ;\u00A0\u00A0\u00A0\ndcterms:identifier<\/a> \"fr<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/www.worldcat.org\/title\/-\/oclc\/960197142<\/a>>\u00A0\u00A0\u00A0\u00A0a \ngenont:InformationResource<\/a>, genont:ContentTypeGenericResource<\/a> ;\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/www.worldcat.org\/oclc\/960197142<\/a>> ; # Luminescence de complexes de lanthanide par effet d\'antenne \u00E0 deux photons : vers l\'imagerie fonctionnelle<\/span>\n\u00A0\u00A0\u00A0\nschema:dateModified<\/a> \"2021-02-17<\/span>\" ;\u00A0\u00A0\u00A0\nvoid:inDataset<\/a> <http:\/\/purl.oclc.org\/dataset\/WorldCat<\/a>> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n\n

Content-negotiable representations<\/p>\n