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Metabolic signaling : methods and protocols

Author: Sarah-Maria Fendt; Sophia Y Lunt
Publisher: New York, N.Y. : Humana Press : Springer, [2018] ©2019
Series: Methods in molecular biology (Clifton, N.J.), v. 1862.
Edition/Format:   eBook : Document : EnglishView all editions and formats

This book provides protocols to quantify metabolism, to identify metabolic crosstalk, and to setup and develop tools and models to gain insight into metabolic signaling using experimental and  Read more...


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Genre/Form: Electronic books
Laboratory Manual
Laboratory manuals
Additional Physical Format: Print version:
Metabolic signaling.
New York, N.Y : Springer, ©2019
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Sarah-Maria Fendt; Sophia Y Lunt
ISBN: 9781493987696 1493987690
OCLC Number: 1056712663
Description: 1 online resource (xii, 294 pages) : illustrations (some color).
Contents: Protocol to compare methods for untargeted metabolomics / Lingjue Wang, Fuad J. Naser, Jonathan L. Spalding, and Gary J. Patti --
High-throughput measure of bioactive lipids using non-targeted mass spectrometry / Kim A. Lagerborg, Jeramie D. Watrous, Susan Cheng, and Mohit Jain--
Measuring the nutrient metabolism of adherent cells in culture / Martin P. Ogrodzinski, Shao Thing Teoh, Lei Yu, Deanna Broadwater, Elliot Ensink, and Sophia Y. Lunt --
¹³C tracer analysis and metabolomics in 3D cultured cancer cells / Marit van Gorsel, Ilaria Elia, and Sarah-Maria Fendt --
Measuring in vivo tissue metabolism using 13C glucose infusions in mice / Dorien Broekaert and Sarah-Maria Fendt --
Measuring human lipid metabolism using deuterium labeling : in vivo and in vitro protocols / Katherine E. Pinnick, Pippa J. Gunn, and Leanne Hodson --
Measuring rates of ATP synthesis / Matthew J. Bird, Silvia Radenkovic, Pieter Vermeersch, and David Cassiman --
Direct estimation of metabolic flux by heavy isotope labeling simultaneous with pathway inhibition : metabolic flux inhibition assay / Tong Zhang, Christiaan F. Labuschagne, Karen H. Vousden, and Oliver D.K. Maddocks --
Measuring glycolytic and mitochondrial fluxes in endothelial cells using radioactive tracers / Koen Veys, Abdiel Alvarado-Diaz, and Katrien De Bock --
Determining compartment-specific metabolic fluxes / Yannic Nonnenmacher, Roberta Palorini, and Karsten Hiller --
Determining the impact of metabolic nutrients on autophagy / Jessica D. Guillaume, Stephanie L. Celano, Katie R. Martin, and Jeffrey P. MacKeigan --
Measuring the activation of cell death pathways upon inhibition of metabolism / Franziska Puschel and Cristina Munoz-Pinedo --
Determining macrophage polarization upon metabolic perturbation / Pu-Ste Liu and Ping-Chih Ho --
Assessing the impact of the nutrient microenvironment on the metabolism of effector CD8+ T cells / Juan Fernandez Garcia and Sarah-Maria Fendt --
Development of patient-derived tumor xenograft models / Daniela Annibali, Eleonora Leucci, Els Hermans, and Frederic Amant --
Imaging glioma progression by intravital microscopy / Fabio Stanchi, Ken Matsumoto, and Holger Gerhardt --
Lipectomizing mice for applications in metabolism / Debrup Chakraborty and Jamie J. Bernard --
Quantitative multiplex immunoassay for profiling bone turnover biomarkers in human bone tissue culture supernatants / Wen-Rong Lie, Derek F. Amanatullah, and Bonnie L. King --
Determining the intracellular organization of organelles / Bruno Latge and Kristine Schauer --
Fundamentals of constructing and interpreting heat maps / Nathaniel M. Vacanti.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1862.
Responsibility: edited by Sarah-Maria Fendt, Sophia Y. Lunt.


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