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Metabolic signaling : methods and protocols

Author: Sarah-Maria Fendt; Sophia Y Lunt
Publisher: New York, N.Y. : Humana Press : Springer, [2018] ©2019
Series: Methods in molecular biology (Clifton, N.J.), v. 1862.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

This book provides protocols to quantify metabolism, to identify metabolic crosstalk, and to setup and develop tools and models to gain insight into metabolic signaling using experimental and  Read more...

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Genre/Form: Electronic books
Laboratory Manual
Laboratory manuals
Additional Physical Format: Print version:
Metabolic signaling.
New York, N.Y : Springer, ©2019
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Sarah-Maria Fendt; Sophia Y Lunt
ISBN: 9781493987696 1493987690
OCLC Number: 1056712663
Description: 1 online resource (xii, 294 pages) : illustrations (some color).
Contents: Protocol to compare methods for untargeted metabolomics / Lingjue Wang, Fuad J. Naser, Jonathan L. Spalding, and Gary J. Patti --
High-throughput measure of bioactive lipids using non-targeted mass spectrometry / Kim A. Lagerborg, Jeramie D. Watrous, Susan Cheng, and Mohit Jain--
Measuring the nutrient metabolism of adherent cells in culture / Martin P. Ogrodzinski, Shao Thing Teoh, Lei Yu, Deanna Broadwater, Elliot Ensink, and Sophia Y. Lunt --
¹³C tracer analysis and metabolomics in 3D cultured cancer cells / Marit van Gorsel, Ilaria Elia, and Sarah-Maria Fendt --
Measuring in vivo tissue metabolism using 13C glucose infusions in mice / Dorien Broekaert and Sarah-Maria Fendt --
Measuring human lipid metabolism using deuterium labeling : in vivo and in vitro protocols / Katherine E. Pinnick, Pippa J. Gunn, and Leanne Hodson --
Measuring rates of ATP synthesis / Matthew J. Bird, Silvia Radenkovic, Pieter Vermeersch, and David Cassiman --
Direct estimation of metabolic flux by heavy isotope labeling simultaneous with pathway inhibition : metabolic flux inhibition assay / Tong Zhang, Christiaan F. Labuschagne, Karen H. Vousden, and Oliver D.K. Maddocks --
Measuring glycolytic and mitochondrial fluxes in endothelial cells using radioactive tracers / Koen Veys, Abdiel Alvarado-Diaz, and Katrien De Bock --
Determining compartment-specific metabolic fluxes / Yannic Nonnenmacher, Roberta Palorini, and Karsten Hiller --
Determining the impact of metabolic nutrients on autophagy / Jessica D. Guillaume, Stephanie L. Celano, Katie R. Martin, and Jeffrey P. MacKeigan --
Measuring the activation of cell death pathways upon inhibition of metabolism / Franziska Puschel and Cristina Munoz-Pinedo --
Determining macrophage polarization upon metabolic perturbation / Pu-Ste Liu and Ping-Chih Ho --
Assessing the impact of the nutrient microenvironment on the metabolism of effector CD8+ T cells / Juan Fernandez Garcia and Sarah-Maria Fendt --
Development of patient-derived tumor xenograft models / Daniela Annibali, Eleonora Leucci, Els Hermans, and Frederic Amant --
Imaging glioma progression by intravital microscopy / Fabio Stanchi, Ken Matsumoto, and Holger Gerhardt --
Lipectomizing mice for applications in metabolism / Debrup Chakraborty and Jamie J. Bernard --
Quantitative multiplex immunoassay for profiling bone turnover biomarkers in human bone tissue culture supernatants / Wen-Rong Lie, Derek F. Amanatullah, and Bonnie L. King --
Determining the intracellular organization of organelles / Bruno Latge and Kristine Schauer --
Fundamentals of constructing and interpreting heat maps / Nathaniel M. Vacanti.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1862.
Responsibility: edited by Sarah-Maria Fendt, Sophia Y. Lunt.

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