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Metalloestrogen cadmium stimulates proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis.
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Metalloestrogen cadmium stimulates proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis.

Author: N Silva Affiliation: Department of Physiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Sri Lanka. Electronic address: yasanga@sjp.ac.lk.; K Tennekoon Affiliation: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo, Sri Lanka.; H Senanayake Affiliation: Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka.; S Samarakoon Affiliation: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo, Sri Lanka.
Edition/Format: Article Article : English
Publication:Taiwanese journal of obstetrics & gynecology, 2013 Dec; 52(4): 540-5
Summary:
OBJECTIVE: To assess the effect of metalloestrogen cadmium (Cd) on the proliferation of endometrial stromal cells (ESC) isolated from the eutopic endometrium of women with endometriosis MATERIALS AND METHODS: ESC were isolated from eutopic endometrial samples from 10 women with endometriosis and 10 women without endometriosis. ESC cultures were established and maintained in RPMI medium. Cultures were treated with Cd  Read more...
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Document Type: Article
All Authors / Contributors: N Silva Affiliation: Department of Physiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Sri Lanka. Electronic address: yasanga@sjp.ac.lk.; K Tennekoon Affiliation: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo, Sri Lanka.; H Senanayake Affiliation: Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka.; S Samarakoon Affiliation: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo, Sri Lanka.
ISSN:1028-4559
Language Note: English
Unique Identifier: 5534576187
Awards:

Abstract:

OBJECTIVE: To assess the effect of metalloestrogen cadmium (Cd) on the proliferation of endometrial stromal cells (ESC) isolated from the eutopic endometrium of women with endometriosis MATERIALS AND METHODS: ESC were isolated from eutopic endometrial samples from 10 women with endometriosis and 10 women without endometriosis. ESC cultures were established and maintained in RPMI medium. Cultures were treated with Cd at a concentration of 10(-6) M and after 24 hours and 48 hours, cell number was counted using the Neubauer hemocytometer to calculate the relative cell proliferation. At 48 hours, the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay was used to test the effect of different concentrations (10(-8) M to 10(-3) M) of Cd on ESC cultures. Relative cell proliferation and MTT assay results were analyzed with ANOVA. RESULTS: At 48 hours, Cd-induced ESC proliferation was higher (p = 0.02) in women with endometriosis than in women without endometriosis, which was confirmed by the MTT assay. CONCLUSION: The results of this study demonstrate the ability of metalloestrogen Cd to induce the proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis.

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