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Methods in enzymology

Author: William A Bradley
Publisher: San Diego : Academic Press, 1996.
Series: Methods in enzymology, 263.
Edition/Format:   Print book : English
This volume provides alternative methods for the quantitation of apolipoproteins and key enzymes in lipoprotein metabolism which can be adapted by investigators with different expertise and laboratory equipment.

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Document Type: Book
All Authors / Contributors: William A Bradley
ISBN: 0121821641 9780121821647
OCLC Number: 422844338
Target Audience: For adults.
Description: 373 pages : illustrations.
Contents: Characterization of Plasma LipoproteinsW. Patsch and A.M. Gotto, Jr., Apolipoproteins: Pathophysiology and Clinical Implications. P. Alaupovic, Significance of Apolipoproteins for Structure, Function, and Classification of Plasma Lipoproteins.Apolipoproteins Apolipoprotein BG.L. Vega and S.M. Grundy, Quantitation of Apolipoprotein B by Chemical Methods. N. Bergeron, L. Kotite, and R.J. Havel, Simultaneous Quantification of Apolipoproteins B-100, B-48, and E Separated by SDS-PAGE. F. Karpe, A. Hamsten, K. Uffelman, and G. Steiner, Apolipoprotein B-48. J.D. Sparks and C.E. Sparks, Chromatographic Method for Isolation and Quantification of Apolipoproteins B-100 and B-48. S.G. Young, E.S. Krul, S. McCormick, R.V. Farese, Jr., and M.F. Linton,Identification and Characterization of Truncated Forms of Apolipoprotein B in Hypobetalipoproteinemia. D.M. Lee, Apolipoprotein B-48: Problems Related to Quantification. R.W. Milne, Immunochemical Separation of Apolipoprotein B-48- and B-100-Containing Lipoproteins.Apolipoprotein EE.S. Krul and T.G. Cole, Quantitation of Apolipoprotein E.Apolipoprotein CP.W. Connelly, C. Vezina, and G.F. Maguire, Quantification of Apolipoprotein C-II by Immunochemical and Chromatographic Methods. M.L. Kashyap, Immunochemical Methods for Quantification of Human Apolipoprotein C-III.Apolipoprotein [a]J.W. Gaubatz, P. Mital, and J.D. Morrisett, Electrophoretic Methods for Quantitation of Lipoprotein [a]. G.M. Fless and M.L. Snyder, Quantitation of Lipoprotein (a) after Lysine-Sepharose Chromatography and Density Gradient Centrifugation. Apolipoprotein A: C.J. Fielding and P.E. Fielding, Two-Dimensional Nondenaturing Electrophoresis of Lipoproteins: Applications to High-Density Lipoprotein Speciation. S.T. Kunitake, P. O'Connor, and J. Naya-Vigne, Heterogeneity of High-Density Lipoproteins and Apolipoprotein A-I as Related to Quantification of Apolipoprotein A-I. G.M. Anantharamaiah and D.W. Garber, Chromatographic Methods for Quantitation of Apolipoprotein A-I. R.B. Weinberg, R.A. Hopkins, and J.B. Jones,Purification, Isoform Characterization, and Quantitation of Human Apolipoprotein A-IV. N.H. Fidge, Immunochemical Methods for Quantification of Apolipoprotein A-IV. Apolipoprotein JS.H. Jenkins, W.D. Stuart, L.A. Bottoms, and J.A.K. Harmony, Quantitation of Plasma Apolipoprotein J.Lipases and Cholesteryl Ester Transfer ProteinR. Potenz, J.-Y. Lo, E. Zsigmond, L.C. Smith, and L. Chan, Human Lipoprotein Lipase: Production in Vitro, Purification, and Generation of Polyclonal Antibody. E. Zsigmond, J.-Y. Lo, L.C. Smith, and L. Chan, Immunochemical Quantitationof Lipoprotein Lipase. A. Bensadoun, Sandwich Immunoassay for Measurement of Human Hepatic Lipase. K.C. Glenn and M.A. Melton, Quantification of Cholesteryl Ester Transfer Protein: Activity and Immunochemical Assay. A. Mitchell and N. Fidge, Determination of Apolipoprotein mRNA Levels by Ribonuclease Protection Assay.
Series Title: Methods in enzymology, 263.
Responsibility: by William A. Bradley ; edited by William A. Bradley, Sandra H. Gianturco, Jere P. Segrest.


Presents methods for the quantitation of apolipoproteins related to cardiovascular disease. This book includes problems of lipoprotein heterogeneity and quantitation. It discusses apolipoproteins  Read more...


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Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE "Incomparably useful." --ANALYTICAL BIOCHEMISTRY "It is a true 'methods' series, including almost Read more...

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