Methods in mouse atherosclerosis (eBook, 2015) []
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Methods in mouse atherosclerosis

Author: Beatriz Dorado; Vicente Andrés
Publisher: New York : Humana Press : Springer, ©2015.
Series: Methods in molecular biology (Clifton, N.J.), v. 1339; Springer protocols (Series)
Edition/Format:   eBook : Document : EnglishView all editions and formats
This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. The chapters catalogue a wide range of in vitro and in vivo experimental methods used for atherosclerosis research, including thorough protocols and  Read more...

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Genre/Form: Electronic books
Additional Physical Format: Print version:
Methods in mouse atherosclerosis.
New York : Humana Press, [2015]
(DLC) 2015948251
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Beatriz Dorado; Vicente Andrés
ISBN: 9781493929290 1493929291 1493929283 9781493929283
OCLC Number: 927456076
Description: 1 online resource (xiv, 400 pages) : illustrations (some color)
Contents: Use of mouse models in atherosclerosis research / Godfrey S. Getz and Catherine A. Reardon --
Cytokines and immune responses in murine atherosclerosis / Pascal J.H. Kusters and Esther Lutgens --
Intravital microscopy for atherosclerosis research / Remco T.A. Megens and Oliver Soehnlein --
Noninvasive molecular imaging of mouse atherosclerosis / Martina A. McAteer and Robin P. Choudhury --
Oil red O and hematoxylin and eosin staining for quantification of atherosclerosis burden in mouse aorta and aortic root / M. Jesús Andrés-Manzano, Vicente Andrés, and Beatriz Dorado --
Isolation, culture, and polarization of murine bone marrow-derived and peritoneal macrophages / Inés Pineda-Torra [and others] --
Isolation of mouse primary aortic endothelial cells by selection with specific antibodies / Pedro Molina-Sánchez and Vicente Andrés --
Isolation and culture of aortic smooth muscle cells and in vitro calcification assay / Ricardo Villa-Bellosta and Magda R. Hamczyk --
Immunostaining of macrophages, endothelial cells, and smooth muscle cells in the atherosclerotic mouse aorta / Prashanthi Menon and Edward A. Fisher --
Immunostaining of lymphocytes in mouse atherosclerotic plaque / Anton Gisterå and Daniel F.J. Ketelhuth --
Flow cytometric analysis of immune cells within murine aorta / Breanne N. Gjurich, Parésa L. Taghavie-Moghadam, and Elena V. Galkina --
In vitro differentiation of naïve CD4+ T cells : a tool for understanding the development of atherosclerosis / Salvador Iborra and Jose M. González-Granado --
Quantification of apoptosis in mouse atherosclerotic lesions / Nichola L. Figg and Martin R. Bennett --
Quantification of cellular proliferation in mouse atherosclerotic lesions / José J. Fuster --
Quantification of in vitro macrophage cholesterol efflux and in vivo macrophage-specific reverse cholesterol transport / Joan Carles Escolà-Gil [and others] --
In vitro macrophage phagocytosis assay / Magda R. Hamczyk, Ricardo Villa-Bellosta, and Vicente Andrés --
Glucose and insulin tolerance tests in the mouse / Ángela Vinué and Herminia González-Navarro --
Wire myography to study vascular tone and vascular structure of isolated mouse arteries / Lara del Campo and Mercedes Ferrer --
Pressure myography to study the function and structure of isolated small arteries / Olav L. Schjørring, Rune Carlsson, and Ulf Simonsen --
Generation of aorta transcript atlases of wild-type and apolipoprotein E-null mice by laser capture microdissection-rased mRNA expression microarrays / Changjun Yin [and others] --
Analysis of gene and protein expression in atherosclerotic mouse aorta by western blot and quantitative real-time PCR / José Rivera-Torres --
Bone marrow transplantation in mice to study the role of hematopoietic cells in atherosclerosis / Vinatha Sreeramkumar and Andrés Hidalgo --
Tandem stenosis to induce atherosclerotic plaque instability in the mouse / Yung Chih Chen, Jennifer Rivera, and Karlheinz Peter --
Detection of intraplaque hemorrhage in mouse atherosclerotic lesions / Judith C. Sluimer, Marion J. Gijbels, and Sylvia Heeneman --
Mechanical stabilization of mouse carotid artery for in vivo intravital microscopy imaging of atherogenesis / Raphaël Chèvre --
Intravital microscopy in the cremaster muscle microcirculation for endothelial dysfunction studies / Cristina Rius and María J. Sanz --
Fluorescent molecular tomography for in vivo imaging of mouse atherosclerosis / Alicia Arranz [and others] --
In vivo 18F-FDG-PET imaging in mouse atherosclerosis / Jesús Mateo [and others] --
Magnetic resonance imaging of the atherosclerotic mouse aorta / Jesús Mateo [and others].
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1339; Springer protocols (Series)
Responsibility: edited by Vicente Andrés, Beatriz Dorado.


This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive  Read more...


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