skip to content
Methods in Tau Cell Biology Preview this item
ClosePreview this item
Checking...

Methods in Tau Cell Biology

Author: Stuart Feinstein; Nichole E Lapointe
Publisher: Cambridge, MA : Academic Press, 2017.
Series: Methods in cell biology, v. 141.
Edition/Format:   eBook : Document : English : First editionView all editions and formats
Summary:
Methods in Tau Cell Biology, Volume 141, the latest release in the Methods in Cell Biology series, looks at methods involved in tau cell biology. Edited by leaders in the field, this volume provides proven, state-of-art techniques and relevant historical background and theory that aids researchers with tactics for efficient design and effective implementation of experimental methodologies. Topics of note in this  Read more...
Rating:

(not yet rated) 0 with reviews - Be the first.

Subjects
More like this

Find a copy online

Links to this item

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Genre/Form: Electronic books
Additional Physical Format: (OCoLC)988300266
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Stuart Feinstein; Nichole E Lapointe
ISBN: 9780128124994 0128124997
OCLC Number: 1002303728
Language Note: Text in English.
Description: 1 online resource (xix, 322 pages) : illustrations.
Contents: Front Cover; Methods in Tau Cell Biology; Copyright; Contents; Contributors; Preface; Section I: Tau Structure, Oligomerization and Aggregation In Vitro; Chapter 1: Expression and isolation of recombinant tau; 1. Introduction; 2. Isolation of Untagged Tau Proteins; 2.1. Preparation of Tau-Expressing E. coli via Transformation; 2.2. (Alternative) Plate Cells From Previously Prepared Glycerol Stock of Transformed E. coli; 2.3. Overnight Starter Cultures; 2.4. Autoinduction of Tau Expression; 2.5. Harvest Bacteria; 2.6. Lyse by French Press; 2.7. Heat Denaturation and High-Speed Centrifugation 2.8. PC Chromatography2.8.1. Column precyclingaaDo this the same day you plan to use the column.; 2.8.2. Batch bind and run column; 2.9. HIC Column; 2.9.1. Equilibrate column 1 day in advance; 2.9.2. HIC column; 2.10. Protein Concentration and Buffer Exchange; 3. Isolation of His-Tagged Tau Proteins; 3.1. Modifications to Early Stages of the Protocol; 3.2. Affinity Chromatography With Ni-NTA Agarose; 3.3. Proteolytic Cleavage to Remove His-Tag; 3.4. Ni-NTA Column for Tag Removal; 4. Determination of Tau Concentration by SDS-PAGE and Comparison to a Tau Mass Standard; Acknowledgements 4.1.1.1. Fitting FCS curves4.2. smFRET Data Collection; 4.2.1. smFRET data analysis; 4.2.1.1. Thresholding; 4.2.1.2. Calculating ETeff; 4.2.1.3. Plotting data and analysis; 4.2.1.4. Calculating distances; 5. Summary; Acknowledgments; References; Chapter 3: Production of recombinant tau oligomers in vitro; 1. Introduction; 2. Purification of Recombinant Tau; 2.1. Growth and Induction of Proteins in E. coli; 2.1.1. Day 1: Transformation of E. coli; 2.1.2. Day 2: Initial Growth of E. coli Culture; 2.1.3. Day 3: Growth and Induction of Proteins in E. coli; 2.2. FPLC Purification of Tau Proteins 2.2.1. Day 1: Purification of Poly-His-Tagged Proteins and Size-Exclusion Chromatography2.2.2. Day 2: Anion Exchange Cleanup of Purified Tau; 2.3. Tau Protein Purification Buffers; 3. SDS-Lowry Protein Quantitation Assay Protocol; 3.1. Reagents; 4. Arachidonic Acid-Induced Formation of Tau Oligomers; 5. Sucrose Step Gradient Fractionation to Isolate Oligomers; 6. Electron Microscopy Imaging of Tau Oligomers; 6.1. Sample Preparation for TEM; 6.2. Materials; 7. Sandwich ELISA for Oligomer Detection; 7.1. TOC1 sELISA Protocol; 7.2. sELISA Quantification; 7.3. Materials; 8. Conclusion
Series Title: Methods in cell biology, v. 141.
Responsibility: edited by Stuart C. Feinstein and Nichole E. Lapointe.

Abstract:

Methods in Tau Cell Biology, Volume 141, the latest release in the Methods in Cell Biology series, looks at methods involved in tau cell biology. Edited by leaders in the field, this volume provides proven, state-of-art techniques and relevant historical background and theory that aids researchers with tactics for efficient design and effective implementation of experimental methodologies. Topics of note in this updated volume include sections on Recombinant tau expression and purification, In vitro MT dynamics and MT ends, Methods related to investigating tau structure and MT bundling, Neurite outgrowth and retraction, and Methods related to studying tau fragmentation.

Reviews

User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.
Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


Primary Entity

<http://www.worldcat.org/oclc/1002303728> # Methods in Tau Cell Biology
    a schema:Book, schema:CreativeWork, schema:MediaObject ;
    library:oclcnum "1002303728" ;
    library:placeOfPublication <http://id.loc.gov/vocabulary/countries/mau> ;
    schema:about <http://experiment.worldcat.org/entity/work/data/4531583204#Topic/tubulins> ; # Tubulins
    schema:about <http://dewey.info/class/571.6/e23/> ;
    schema:about <http://experiment.worldcat.org/entity/work/data/4531583204#Topic/science_life_sciences_anatomy_&_physiology> ; # SCIENCE / Life Sciences / Anatomy & Physiology
    schema:about <http://experiment.worldcat.org/entity/work/data/4531583204#Topic/cytology> ; # Cytology
    schema:bookEdition "First edition." ;
    schema:bookFormat schema:EBook ;
    schema:datePublished "2017" ;
    schema:description "Methods in Tau Cell Biology, Volume 141, the latest release in the Methods in Cell Biology series, looks at methods involved in tau cell biology. Edited by leaders in the field, this volume provides proven, state-of-art techniques and relevant historical background and theory that aids researchers with tactics for efficient design and effective implementation of experimental methodologies. Topics of note in this updated volume include sections on Recombinant tau expression and purification, In vitro MT dynamics and MT ends, Methods related to investigating tau structure and MT bundling, Neurite outgrowth and retraction, and Methods related to studying tau fragmentation."@en ;
    schema:description "Front Cover; Methods in Tau Cell Biology; Copyright; Contents; Contributors; Preface; Section I: Tau Structure, Oligomerization and Aggregation In Vitro; Chapter 1: Expression and isolation of recombinant tau; 1. Introduction; 2. Isolation of Untagged Tau Proteins; 2.1. Preparation of Tau-Expressing E. coli via Transformation; 2.2. (Alternative) Plate Cells From Previously Prepared Glycerol Stock of Transformed E. coli; 2.3. Overnight Starter Cultures; 2.4. Autoinduction of Tau Expression; 2.5. Harvest Bacteria; 2.6. Lyse by French Press; 2.7. Heat Denaturation and High-Speed Centrifugation"@en ;
    schema:editor <http://experiment.worldcat.org/entity/work/data/4531583204#Person/feinstein_stuart_1954> ; # Stuart Feinstein
    schema:editor <http://experiment.worldcat.org/entity/work/data/4531583204#Person/lapointe_nichole_e> ; # Nichole E. Lapointe
    schema:exampleOfWork <http://worldcat.org/entity/work/id/4531583204> ;
    schema:genre "Electronic books"@en ;
    schema:inLanguage "en" ;
    schema:isPartOf <http://experiment.worldcat.org/entity/work/data/4531583204#Series/methods_in_cell_biology> ; # Methods in cell biology ;
    schema:isSimilarTo <http://www.worldcat.org/oclc/988300266> ;
    schema:name "Methods in Tau Cell Biology"@en ;
    schema:productID "1002303728" ;
    schema:url <http://public.ebookcentral.proquest.com/choice/publicfullrecord.aspx?p=4987819> ;
    schema:url <http://lib.myilibrary.com?id=1031912> ;
    schema:url <http://proxy.library.brocku.ca/login?url=http://www.sciencedirect.com/science/bookseries/0091679X/141> ;
    schema:url <http://dproxy.library.dc-uoit.ca/login?url=https://www.sciencedirect.com/science/bookseries/0091679X/141> ;
    schema:url <http://www.sciencedirect.com/science/bookseries/0091679X/141> ;
    schema:url <http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=1483394> ;
    schema:workExample <http://worldcat.org/isbn/9780128124994> ;
    umbel:isLike <http://bnb.data.bl.uk/id/resource/GBB7C4037> ;
    wdrs:describedby <http://www.worldcat.org/title/-/oclc/1002303728> ;
    .


Related Entities

<http://dproxy.library.dc-uoit.ca/login?url=https://www.sciencedirect.com/science/bookseries/0091679X/141>
    rdfs:comment "eBook available for Durham College via Elsevier. Click link to access" ;
    .

<http://experiment.worldcat.org/entity/work/data/4531583204#Person/feinstein_stuart_1954> # Stuart Feinstein
    a schema:Person ;
    schema:birthDate "1954" ;
    schema:familyName "Feinstein" ;
    schema:givenName "Stuart" ;
    schema:name "Stuart Feinstein" ;
    .

<http://experiment.worldcat.org/entity/work/data/4531583204#Person/lapointe_nichole_e> # Nichole E. Lapointe
    a schema:Person ;
    schema:familyName "Lapointe" ;
    schema:givenName "Nichole E." ;
    schema:name "Nichole E. Lapointe" ;
    .

<http://experiment.worldcat.org/entity/work/data/4531583204#Series/methods_in_cell_biology> # Methods in cell biology ;
    a bgn:PublicationSeries ;
    schema:hasPart <http://www.worldcat.org/oclc/1002303728> ; # Methods in Tau Cell Biology
    schema:name "Methods in cell biology ;" ;
    .

<http://experiment.worldcat.org/entity/work/data/4531583204#Topic/science_life_sciences_anatomy_&_physiology> # SCIENCE / Life Sciences / Anatomy & Physiology
    a schema:Intangible ;
    schema:name "SCIENCE / Life Sciences / Anatomy & Physiology"@en ;
    .

<http://worldcat.org/isbn/9780128124994>
    a schema:ProductModel ;
    schema:isbn "0128124997" ;
    schema:isbn "9780128124994" ;
    .


Content-negotiable representations

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.