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Microarrays & microplates : applications in biomedical sciences

Author: S Ye; Ian N M Day
Publisher: Oxford, UK : BIOS ; New York : Distributed in the U.S.A. by Springer-Verlag, 2003.
Series: Advanced methods.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

This title looks at the new microarray and microplate-based technologies which facilitate large-scale analysis of DNA sequence variants, mRNA molecules and proteins.

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Details

Genre/Form: Laboratory Manual
Document Type: Book
All Authors / Contributors: S Ye; Ian N M Day
ISBN: 9781859960745 185996074X
OCLC Number: 51032550
Description: ix, 178 pages : illustrations ; 25 cm.
Contents: Instrumentation for Genomics and Proteomics / Sarah K. Stephens, Mark R. Truesdale, Julian F. Burke --
History of the microplate --
Microplate-handling instrumentation --
Applications in genomics --
Applications in proteomics --
Arraying of PCR products onto nylon membranes --
Processing colony arrays --
Expression Microarrays / Carl Whatling, Shu Ye, Per Eriksson --
Array fabrication --
Preparation of labelled probes --
Microarray hybridization --
Generation and analysis of expression data --
The need for databases --
Validation of gene expression changes --
PCR amplification of cDNAs --
PCR product purification by precipitation --
Post-processing of glass slides --
Fluorescent probe labelling --
Pre-hybridization --
Microarray hybridization and washing --
Tissue Microarrays: A High-Throughput Technology for Translational Research / Susan Henshall --
Tissue microarray construction --
Staining of tissue microarrays --
Tissue microarray data and image management --
Applications in translational research --
Validation of tissue microarrays --
Future developments --
Protein Chips and Microarrays / C. David O'Connor, Karen Pickard --
Microarray technology --
Versatility of protein microchips --
Single Nucleotide Polymorphism Genotyping Using Microarrays / Tomi Pastinen --
DNA microarrays for sequence analysis --
Arrays for resequencing and SNP-scoring --
Multiplexed allele-specific extension coupled with tag-arrays --
Microplate-Based mRNA Analysis / Taku Murakami, Masato Mitsuhashi --
Microplate-based RNA purification.
Series Title: Advanced methods.
Other Titles: Microarrays and microplates
Responsibility: S. Ye & I.N.M. Day (eds.).
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