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Microinjection

Author: Juan Carlos Lacal; Rosario Perona; James Feramisco
Publisher: Basel ; Boston : Birkhäuser Verlag, ©1999.
Series: Methods and tools in biosciences and medicine.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:
"The technique of needle microinjection has become a prominent experimental approach in biological research."--BOOK JACKET. "This book presents information on the technique's principles, the required equipment, preparation of recipient cells and injected material and the analysis of the results. It provides detailed protocols for a wide range of important applications in different cellular systems from the fields of  Read more...
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Document Type: Book
All Authors / Contributors: Juan Carlos Lacal; Rosario Perona; James Feramisco
ISBN: 0817659730 9780817659738 3764359730 9783764359737 0817660194 9780817660192 3764360194 9783764360191
OCLC Number: 40331082
Description: 248 pages : illustrations ; 25 cm.
Contents: 1. Needle Microinjection: A Brief History / J. Feramisco, R. Perona and J.C. Lacal --
2. Microinjection of Macromolecules into Mammalian Cells in Culture / R. Perona, F. Dolfi and J. Feramisco / [and others] --
3. The Use of Plasmid Microinjection to Study Specific Cell Cycle Phase Transitions / E.S. Knudsen --
4. Combining Microinjection with Microanalytical Methods to Investigate Regulation of Metabolic Pathways / J. Manchester and J.C. Lawrence, Jr. --
5. Import of Stably Folded Proteins into Peroxisomes / P.A. Walton --
6. Study of the Function of Microtubule Proteins / R. Armas-Portela and J. Avila --
7. Cytoskeleton Regulation by Rho Proteins / R. Kozma, S. Ahmed and L. Lim --
8. Cytoskeletal Regulation by Rho-p21 Associated Kinases: Analysis of ROK and PAK Function by Plasmid Microinjection / T. Leung, E. Manser and L. Lim --
9. Use of Microinjection to Study Apoptosis and its Prevention / F. Kohlhuber, H. Hermeking and D. Eick --
10. Role of the InsPase and Casuperscript 2+] Entry / R.S. Mathias and H.E. Ives --
11. Microinjection of Xenopus laevis Oocytes: A Model System / J.C. Lacal --
12. Xenopus laevis Oocytes as a Model for Studying the Activation of Intracellular Kinases / A. Carnero and J.C. Lacal --
13. Signal Transduction in Xenopus laevis Oocytes by Ras Oncoproteins and Lipid Metabolites / A. Carnero and J.C. Lacal --
14. The Multiple Roles of Mos During Meiosis / M.S. Murakami and G.F. Vande Woude --
15. Use of the Xenopus Oocyte System to Study RNA Transport / L.A. Allison --
16. Functional Expression of G Protein-Coupled Receptors in Xenopus laevis Oocytes / P. de la Pena and F. Barros --
17. Expression of Glucose Transporters in Xenopus laevis Oocytes: Applications for the Study of Membrane Transporter Function and Regulation / M.J. Seatter and G.W. Gould --
18. DNA Injection into Xenopus laevis Embryos as a Tool to Study Spatial Gene Activity / M. Kuhl, M. Walter and J. Clement / [and others]. 19. Transformation of Nematodes by Microinjection / S. Hashmi, G. Hashmi and R. Gaugler --
Guide to Solutions --
Guide to Protocols --
Troubleshooting Guide.
Series Title: Methods and tools in biosciences and medicine.
Responsibility: edited by Juan Carlos Lacal, Rosario Perona, James Feramisco.

Abstract:

This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. Information is presented on the  Read more...

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