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Minicircle and miniplasmid DNA vectors : the future of nonviral and viral gene transfer

Author: M Schleef
Publisher: Weinheim, Germany : Wiley-Blackwell, [2013] ©2013
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

* Covers a new approach to gene therapy with a greatly improved safety profile * Complete coverage of the topic including molecular basis, production and biomedical applications * Has a wide  Read more...

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Document Type: Book
All Authors / Contributors: M Schleef
ISBN: 9783527324569 3527324569
OCLC Number: 843345752
Description: xxi, 235 pages : illustrations (black and white, and colour) ; 25 cm
Contents: The History of Minicircle (Martin Grund, Martin Schleef) Operator-Repressor Titration: Stable Plasmid Maintenance without Selectable Marker Genes (Rocky M. Cranenburgh) Selection by RNA/RNA Interaction: Maximally Minimized Antibiotic-Resistance Free Plasmids (Reingard Grabherr, Jurgen Mairhofer) Plasmid-Based Medicinal Products - Focus on pFAR: A Miniplasmid Free of Antibiotic Resistance Markers (Daniel Scherman, Corinne Marie, Mickael Quiviger, Helen Foster, George Dickson) Plasmid DNA Concatemers - Influence of Plasmid Structure on Transfection Efficiency (Christof Maucksch, Bronwen Connor, Carsten Rudolph) Analytical Tools in Minicircle Production (Martin Schleef, Anja Rischmuller, Martina Viefhues, Mareike Dieding, Markus Blaesen, Marco Schmeer, Ruth Baier, Dario Anselmetti) Utilising Minicircle Vectors for the Episomal Modification of Cells (Richard P. Harbottle, Orestis Argyros, Suet-Ping Wong, Charles Coutelle) Replicating Minicircles: Overcoming the Limitations of Transient and of Stable Expression Systems (Jurgen Bode, Kristina Nehlsen, Sandra Broll, Raju Kandimalla, Niels Heinz, Markus Heine, Stefanie Binius, Axel Schambach) Magnetofection of Minicircle DNA Vectors (Olivier Zelphati, Flavie Sicard, Cedric Sapet, Nicolas Laurent, Elodie Bertosio, Melanie Bertuzzi) Minicircle Based Vectors for Non-Viral Gene Therapy: in vitro Characterization and in vivo Application (Dennis Kobelt, Jutta Aumann, Martin Schleef, Marco Schmeer, Ulrike Stein, Peter M. Schlag, Wolfgang Walther) Episomal Expression of Minicircles and Conventional Plasmids in Mammalian Embryos (Wilfried A. Kues, Wiebke Garrels, Khursheed Iqbal) Tissue Targeted Gene Electro-delivery of Minicircle DNA (Sophie Chabot, Muriel Golzio, Justin Teissie) Increased Efficiency of Minicircles vs Plasmids under Gene Electrotransfer Suboptimal Conditions: An Influence of the Extracellular Matrix (V. Joubert, Franck M. Andre, Marco Schmeer, Martin Schleef, Lluis M. Mir)
Responsibility: edited by Martin Schleef.

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