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Molecular cloning : a laboratory manual

Author: Joseph Sambrook; David W Russell
Publisher: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, ©2001.
Edition/Format:   Print book : English : 3rd edView all editions and formats
Summary:
DSU Title III 2002-2012.
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Genre/Form: Laboratory manuals
Laboratory Manual
Manuels de laboratoire
Manuales de laboratorio
Additional Physical Format: Online version:
Sambrook, Joseph.
Molecular cloning.
Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, ©2001
(OCoLC)606593834
Online version:
Sambrook, Joseph.
Molecular cloning.
Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, ©2001
(OCoLC)608404168
Document Type: Book
All Authors / Contributors: Joseph Sambrook; David W Russell
ISBN: 0879695773 9780879695774 0879695765 9780879695767 9780879698140 0879698144
OCLC Number: 45015638
Description: 3 volumes : illustrations ; 28 cm
Contents: Vol. 1: Ch. 1. Plasmids and their usefulness in molecular cloning --
Ch. 2. Bacteriophage and its vectors --
Ch. 3. Working with bacteriophage M13 vectors --
Ch. 4. Working with high-capacity vectors --
Ch. 5. Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis --
Ch. 6. Preparation and analysis of eukaryotic genomic DNA --
Ch. 7. Extraction, purification, and analysis of mRNA from eukaryotic cells --
Vol. 2: Ch. 8. In Vitro amplification of DNA by the polymerase chain reaction --
Ch. 9. Preparation of radiolabeled DNA and RNA probes --
Ch. 10. Working with synthetic oligonucleotide probes --
Ch. 11. Preparation of cDNA libraries and gene identification --
Ch. 12. DNA sequencing --
Ch. 13. Mutagenesis --
Ch. 14. Screening expression libraries --
Vol. 3: Ch. 15. Expression of cloned genes in Escherichia coli --
Ch. 16. Introducing cloned genes into cultured mammalian cells --
Ch. 17. Analysis of gene expression in cultured mammalian cells --
Ch. 18. Protein interaction technologies.
Responsibility: Joseph Sambrook, David W. Russell.

Abstract:

DSU Title III 2002-2012.

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