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Molecular imaging : FRET microscopy and spectroscopy

Author: Ammasi Periasamy; Richard N Day; American Physiological Society (1887- )
Publisher: Oxford ; New York : Published for the American Physiological Society by Oxford University Press, 2005.
Series: Methods in physiology series.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an  Read more...
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Genre/Form: Electronic book
Electronic books
Additional Physical Format: Print version:
Molecular imaging.
Oxford ; New York : Published for the American Physiological Society by Oxford University Press, 2005
(DLC) 2004061702
(OCoLC)57406048
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Ammasi Periasamy; Richard N Day; American Physiological Society (1887- )
ISBN: 9780195177206 0195177207 9780080536873 0080536875
OCLC Number: 162131550
Reproduction Notes: Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010. MiAaHDL
Description: 1 online resource (xv, 312 pages) : illustrations (some color).
Details: Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Contents: Proteins and the Flow of Information in Cellular Function; Basics of Fluorescence and FRET; An Introducrion to Filters and Mirrors for FRET; FRET Imaging in the Wide-field Microscope; Confocal FRET Microscopy --
Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes; Multiphoton FRET Microscopy for Protein Localization in Tissue; Single-Molecule FRET; FRET Measurements using Multispectral Imaging; Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers; Streak FLIM: A Novel Technology for Quantitative FRET Imaging; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization;Bioluminescence RET (BRET): Techniques and Potential; Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy; Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization.
Series Title: Methods in physiology series.
Responsibility: edited by Ammasi Periasamy, Richard N. Day.
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Abstract:

FRET microscopy and spectroscopy techniques provide the tools for measuring protein interactions within living cells at the nanomolecular level. This book presents the fundamental principles behind  Read more...

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"This is a gold mine of information for those interested in using FRET microscopy to study protein-protein interactions. A major strength of this book is that it is focused and yet Read more...

 
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