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Monoclonal antibodies : a manual of techniques

Author: Heddy Zola
Publisher: Boca Raton, Fl. : CRC Press, 1987.
Edition/Format:   eBook : Document : EnglishView all editions and formats
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Zola, Heddy.
Monoclonal antibodies.
Boca Raton, Fl. : CRC Press, 1987
(DLC) 86018394
(OCoLC)13947311
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Heddy Zola
ISBN: 9781482242683 1482242680 9780429165313 0429165315
OCLC Number: 1048938182
Description: 1 online resource
Contents: Cover; Title Page; Copyright Page; Contents; Chapter 1: Introduction; I. Aim and Scope of This Book; II. Antibody as a Specific Probe; III. Monoclonal Antibody; IV. Monoclonal and Polyclonal Antibody Compared; A. One Antibody or Many Different Antibodies; B. Different Antibodies Against the Same Antigen; C. Single or Multiple Antigenic Epitopes; D. Recognition of Patterns Consisting of Several Antigenic Determinants; E. Sensitivity and Specificity; F. Labile Epitopes; G. Labile Antibodies; H. Affinity; I. Cross Reactivity; V. Monoclonal Antibodies as Standard Reagents VI. Comments on Publication, Supply, and Commercialization of Monoclonal AntibodiesReferences; Chapter 2: Overview; I. Aim of the Chapter; II. Applications and Limitations of Monoclonal Antibodies (Do I Need Monoclonals, and What Can I Realistically Expect to Do With Them?); A. Identification and Quantitation of a Soluble Molecule in a Mixture; B. Purification of a Soluble Molecule from a Mixture; C. Detection and Quantitation of a Cell Type in Suspension; D. Separation of a Particular Cell Type from a Mixture; E. Identification of Molecular and Cellular Structures in Tissue F. Clinical ApplicationsIII. Availability (Shall I Make My Own Monoclonal Antibodies or Can I Obtain Them?); IV. Resources Needed to Make Monoclonal Antibodies; A. Major Equipment; 1. Cabinet for Sterile Work; 2. Gassed Incubator; 3. Liquid Nitrogen Facility; 4. Animal House; B. Minor Equipment; C. Staff and Skills Required; References; Further Reading; Chapter 3: Making Hybridomas; I. Scope of the Chapter; II. Strategy; III. Materials to be Ordered; A. Quantities Needed; B. Description; 1. Cell Culture Medium: RPMI 1640 or Equivalent; 2. Fetal Calf Serum (FCS) or Other Serum 3. HAT Selective Medium Components4. Dimethyl Sulfoxide; 5. Polyethylene Glycol (PEG); IV. Stock and Working Solutions; A. Medium; B. HAT Medium; C. HT Medium; D. Geys Hemolytic Medium; 1. Geys Solution A; 2. Geys Solution B; 3. Geys Solution C; 4. Working Solution; E. Phosphate-Buffered Saline (PBS); F. PEG/DMSO Fusing Agent Solution; V. Immunization; A. The Antibody Response; B.A "Typical" Immunization Protocol; C. List of Variables; D. Detailed Analysis of Variables; 1. Choice of Animal; 2. Antigen; 3. Dose, Adjuvant, Route; 4. Booster Injections; 5. Improving Specificity 6. Serum Antibody to Assess Immunization7. Local Immune Stimulation; VI. Hybridization; A. The Myeloma Cell Line; B. Spleen Cells; C. The Fusion Process; 1. Introduction and Rationale; 2. Materials and Preparation; 3. Procedure; 4. Notes; VII. Maintenance and Propagation; A. Introduction and Rationale; B. Culture of Hybrids after Fusion; VIII. Screening; A. Introduction and Rationale; 1. Primary and Secondary Interactions; 2. Single and Multiple Determinants; 3. The Prozone Phenomenon; 4. Sensitivity and Background Staining; 5. Speed and Convenience; B. Specific Applications and Examples
Responsibility: author, Heddy Zola.

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