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Mutagenicity.

Author: Ashutosh Kumar; Vasily N Dobrovolsky; Alok Dhawan; Rishi Shanker
Publisher: Saint Louis : Elsevier Science, 2017.
Edition/Format:   eBook : Document : EnglishView all editions and formats
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Kumar, Ashutosh.
Mutagenicity: Assays and Applications.
Saint Louis : Elsevier Science, ©2017
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Ashutosh Kumar; Vasily N Dobrovolsky; Alok Dhawan; Rishi Shanker
ISBN: 9780128092606 0128092602
OCLC Number: 1005613269
Notes: ACKNOWLEDGMENT.
Description: 1 online resource (352 pages)
Contents: Front Cover; MUTAGENICITY: ASSAYS AND APPLICATIONS ; MUTAGENICITY: ASSAYS AND APPLICATIONS ; Copyright; CONTENTS; LIST OF CONTRIBUTORS; FOREWORD; PREFACE; ACKNOWLEDGMENTS; 1 --
Mutagenesis, Genetic Disorders and Diseases; 1. INTRODUCTION; 2. MUTAGENS; 2.1 Physical Mutagens; 2.2 Chemical Mutagens; 2.3 Biological Mutagens; 3. MUTATIONS; 3.1 Break in Homologous Chromosome; 3.2 Breaks in Nonhomologous Chromosomes; 4. DNA DAMAGE RESPONSE AND REPAIR; 5. DNA REPAIR PATHWAYS; 5.1 Direct Repair; 5.2 Single-Strand Break Repair; 5.2.1 Base Excision Repair; 5.2.2 Nucleotide Excision Repair. 5.2.3 Mismatch Repair5.2.4 Interstrand Cross-Linking Repair; 5.3 Double-Strand DNA Breaks Repair; 6. GENETIC DISORDERS AND DISEASES; 6.1 Noonan Syndrome (OMIM 163950); 6.2 Costello Syndrome (OMIM 218040); 6.3 Cardio-Facio-Cutaneous Syndrome (OMIM 115150); 6.4 Hirschsprung Disease (OMIM 142623); 6.5 Hutchinsons-Gilford Progeria Syndrome (OMIM 176670); 6.6 Cancer; 6.7 Parkinson Disease (OMIM 168600); 6.8 Alzheimer Disease (OMIM 104300); 6.9 Ataxia-Telangiectasia (OMIM 208900); 6.10 Seckel Syndrome (OMIM 210600); 6.11 Cockayne Syndrome (OMIM 216400); 6.12 Fanconi Anemia (OMIM 227650). 6.13 Bloom Syndrome (OMIM 210900)6.14 Werner Syndrome (OMIM 277700); 6.15 Xeroderma Pigmentosum (OMIM 278700); 7. SUMMARY; ACKNOWLEDGMENT; REFERENCES; 2 --
Detection of Mutation in Prokaryotic Cells; 1. INTRODUCTION; 2. MATERIALS AND METHODOLOGIES; 2.1 Preparation of Mammalian Liver S9 Fraction; 2.1.1 Induction of Rat Liver Enzymes; 2.1.2 Removal of Liver From Rats; 2.1.3 Preparation of Liver S9 Fraction; 2.2 Preparation of Reagents; 2.2.1 Vogel-Bonner (VB Salt) Solution (50×); 2.2.2 Glucose Solution (10% W/V); 2.2.3 Minimal Glucose Agar Plates; 2.2.4 Histidine-Biotin Solution (0.5mM). 2.2.5 Top Agar Supplemented With Histidine-Biotin Solution2.2.6 Buffers for Metabolic Activation; 2.2.6.1 Sodium Phosphate Buffer (0.1mM, pH 7.4); 2.2.6.2 To Activate the NADP Regenerating System in Presence of Liver S9 Fraction; 2.3 Methodology; 2.3.1 Culture Preparation; 2.3.2 Genetic Analysis of the Tester Strains; 2.3.3 Histidine and Biotin (his, bio) or Tryptophan (trp) Dependence; 2.3.4 rfa (Deep Rough) Mutation; 2.3.5 Treatment of Culture; 2.3.6 Plating of Treated Culture; 3. RESULT EVALUATION; 4. RESULT INTERPRETATION; 5. EXPERIMENTAL DESIGN; 6. PRECAUTION; REFERENCES; FURTHER READING. 3 --
Detection of Gene Mutation in Cultured Mammalian Cells1. INTRODUCTION; 2. PCR-BASED MUTATION DETECTION; 3. DETECTION OF MUTATIONS BY DENATURING GRADIENT GEL ELECTROPHORESIS; 4. SINGLE-STRAND CONFORMATION POLYMORPHISM; 5. HETERODUPLEX ANALYSIS; 6. MICROARRAY; 7. ARRAYED PRIMER EXTENSION TECHNOLOGY; 8. SINGLE-BASE EXTENSION-TAGS TECHNOLOGY; 9. ATOMIC FORCE MICROSCOPY; 10. FLUORESCENCE IN SITU HYBRIDIZATION; 11. DNA SEQUENCING; 12. KARYOTYPING; 13. HYPOXANTHINE PHOSPHORIBOSYL TRANSFERASE, THYMIDINE KINASE, AND XANTHINE-GUANINE PHOSPHORIBOSYL TRANSFERASE GENE MUTATION DET ... ; 14. CONCLUSION.

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