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Nuclear magnetic resonance

Author: H F Linskens; J F Jackson; C Abell
Publisher: Berlin ; New York : Springer-Verlag, ©1986.
Series: Modern methods of plant analysis, new ser., v. 2.
Edition/Format:   Print book : EnglishView all editions and formats
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Additional Physical Format: Online version:
Nuclear magnetic resonance.
Berlin ; New York : Springer-Verlag, ©1986
(OCoLC)610543767
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: H F Linskens; J F Jackson; C Abell
ISBN: 038715910X 9780387159102 354015910X 9783540159100
OCLC Number: 13095988
Description: xi, 196 pages : illustrations ; 25 cm.
Contents: 13C-NMR in Metabolic Studies.- 1 Introduction.- 2 The Design and Interpretation of Labelling Experiments.- 3 Radioisotope Labelling.- 4 13C NMR Spectroscopy.- 4.1 Assignment of 13C NMR Spectra.- 4.1.1 Known Chemical Shifts and Substituent Chemical Shift Effects.- 4.1.2 Off-Resonance and Specific Proton Decoupling.- 4.1.3 Analysis of Long-Range 1H-13C Couplings.- 4.1.4 Polarisation Transfer and 2D NMR Methods.- 4.1.5 Lanthanide-Induced Shift Studies.- 4.1.6 Model and Derivative Studies.- 4.1.7 Synthesis of Isotopically Labelled Compounds.- 4.1.8 Incorporation Studies.- 5 Biosynthetic Methodology.- 5.1 Precursor Incorporation.- 6 13C Enrichment Studies.- 6.1 Single 13C-Labelling.- 6.1.1 Quantitative 13C Measurements.- 6.1.2 Single 13C-Labelling Studies in Plants.- 6.2 13C-13C Spin-Spin Coupling.- 6.2.1 Singly 13C-Labelled Precursors.- 6.2.2 Double 13C-Labelled Precursors.- 6.2.3 Non-Contiguous Double 13C-Labelling.- 6.2.3.1 Two-Bond 13C-13C Couplings from Contiguous 13C2-Labelled Precursors.- 6.2.3.2 One-Bond 13C-13C Couplings from Non-Contiguous 13C2-Labelled Precursors.- 6.2.3.3 Two-Bond Couplings from Non-Contiguous 13C2-Labelled Precursors.- 7 13C, 15N Doubly Labelled Precursors.- 8 Isotope-Induced Shifts in 13C NMR.- 8.1 The Deuterium Alpha-Shift Technique.- 8.2 The Deuterium Beta-Shift Technique.- 8.3 18O Isotope Induced Shifts in 13C NMR.- 9 Conclusion.- References.- Determination of the Energy Status of Plant Cells by 31P-Nuclear Magnetic Resonance Spectroscopy.- 1 Introduction.- 2 Principles.- 3 Experimental Considerations.- 4 Quantitation of Metabolite Concentrations of vivo by NMR.- 5 Measurement of Nucleoside Di- and Triphosphate Levels by 31P-NMR.- 6 Estimation of the Extent of Magnesium Ion Binding to Nucleoside Triphosphates in vivo by 31P-NMR.- 7 Measurement of the Gibbs' Free Energy for Hydrolysis of ATP.- 8 Relationship of in vivo 31P-NMR Measurements to What Is Seen by Other Methods.- 9 Conclusions.- References.- The Use of NMR Spectroscopy to Follow Deuterium in Studies of Fungal Metabolism.- 1 Introduction.- 2 Deuterium NMR Spectroscopy.- 2.1 Background and Techniques.- 2.1.1 The Nuclear Magnetic Properties of Deuterium.- 2.1.2 The Deuterium NMR Spectrum.- 2.1.3 Recording a Deuterium NMR Spectrum.- 2.2 Applications of Deuterium NMR Spectroscopy to Fungal Metabolism.- 2.2.1 Administering the Precursor.- 2.2.2 Establishing the Retention or Loss of a Hydrogen.- 2.2.3 Establishing Precursor-Product Relationships.- 2.2.4 Mechanistic Studies.- 2.2.4.1 Displacement Reactions.- 2.2.4.2 Incorporation of Deuterium Atoms from the Medium.- 2.2.4.3 Following Hydride Shifts.- 2.2.5 The Use of [2-2H3]-Acetate as a Polyketide Precursor..- 2.3 Kinetic Isotope Effects.- 2.4 The Use of Shift Reagents.- 3 Detection of Deuterium Indirectly in the 13C-NMR Spectrum.- 3.1 Background and Techniques.- 3.1.1 Detecting Deuterium Directly Attached to a Carbon Reporter Nucleus (the ?-Shift Technique).- 3.1.2 Detecting Deuterium on a Carbon Adjacent to the Reporter Nucleus (the ?-Shift Technique).- 3.2 Applications to the Study of Biosynthesis in Fungi.- 3.2.1 Quantitative Measurements of Deuterium Retention.- 3.2.2 Determining the Multiplicity of Labelling.- 3.2.3 Labelling a Bond.- 4 Conclusions.- References.- Proton NMR Studies on DNA Structure.- 1 Introduction.- 2 Theoretical Background.- 2.1 Chemical Shifts.- 2.2 Coupling Constants.- 2.3 NOE.- 2.4 Assignments.- 3 Examples.- 4 Summary.- References.- NMR Methods for Determination of Intracellular pH.- 1 Introduction.- 2 Some Principles.- 3 Determinants of Chemical Shift.- 4 Measurement of Chemical Shifts.- 5 Estimating Intracellular pH from Chemical Shifts: Titration Curves.- 6 Other Factors Contributing to Potential Errors in Intracellular pH Measurements Using NMR.- 7 Observation of pH Heterogeneity in Cell Populations and Tissues by NMR.- 8 NMR Signals That Have Been Used as Intracellular pH Indicators.- 9 Assignment of Resonances.- 10 Can the pH in Compartments Other Than Cytoplasm and Vacuole be Measured in Vivo by NMR Methods?.- 11 Conclusions.- References.- Orientation of Chloroplasts in Leaves by 1H NMR Spectroscopy.- 1 Introduction.- 1.1 What is NMR?.- 1.2 NMR Spectra of Plant Leaves.- 2 Experimental Technique.- 2.1 Choosing an NMR Spectrometer.- 2.2 Sample Holders.- 2.3 Sample Preparation.- 2.4 Operating the Spectrometer.- 3 Data Analysis.- 3.1 The Need for a Theory.- 3.2 Review of Leaf Anatomy and Physiology.- 3.3 The Theory.- 3.4 The Model.- 3.5 Calculations.- 4 Examples of Interpreted Spectra.- 4.1 Orientation Dependence.- 4.2 Species Dependence.- 4.3 Effects of Drying.- 4.4 Effects of Freezing.- 5 Future Directions.- References.- 13C-NMR Determination of Rubber Content in Guayule Bushes.- 1 Introduction.- 1.1 Background.- 1.2 Analytical Methods.- 2 Experimental.- 2.1 13C-NMR Spectra.- 2.2 Sample Preparation.- 2.2.1 Guayule Material.- 2.2.2 Sample Geometry.- 2.3 Calibration Curve.- 3 Results and Discussion.- 3.1 13C-NMR Spectra.- 3.2 Sample Preparation.- 3.2.1 Guayule Sample.- 3.2.2 Sample Geometry.- 3.2.2.1 Calibration Plot.- 3.3 Quantitative Results.- 3.3.1 Milled Bush.- 3.3.1.1 Precision of the Method.- 3.3.2 Guayule Branches.- 3.3.3 Branches of Different Sizes; Bark.- 3.3.4 Branches, Trunk, Roots.- 3.3.4.1 Extraction Options.- 4 Conclusions.- Appendix A 13C-NMR Method.- References.- Nuclear Magnetic Resonance and Pollen Quality.- 1 What is the Pollen Grain?.- 1.1 Organization and Role.- 1.2 Water Content, Membrane Organization and Pollen Viability.- 2 1H-NMR - Water Content.- 2.1 Method.- 2.2 General.- 2.3 Water Content Evolution.- 2.4 Water State Evolution.- 2.4.1 Relaxation Time.- 2.4.2 Hydric Compartmentation.- 2.5 Dehydration Control and Cryptobiotic Organisms.- 3 31P, 13C-NMR and Membrane State.- 3.1 Structural Polymorphism.- 3.2 Functional Aspects of Membrane Polymorphism.- 3.2.1 Hexagonal Configuration: HII.- 3.2.2 Vesicular, Micellar or Inverted Micellar Configuration..- 3.2.3 Application to Water Stress.- 3.3 13C-NMR and Membrane State.- 4 Conclusions.- References.
Series Title: Modern methods of plant analysis, new ser., v. 2.
Responsibility: edited by H.F. Linskens and J.F. Jackson ; contributors, C. Abell [and others].

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<http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Topic\/nmr_spektroskopie<\/a>> # NMR-Spektroskopie<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"NMR-Spektroskopie<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Topic\/nuclear_magnetic_resonance<\/a>> # nuclear magnetic resonance<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"nuclear magnetic resonance<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Topic\/nuclear_magnetic_resonance_spectroscopy<\/a>> # nuclear magnetic resonance spectroscopy<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"nuclear magnetic resonance spectroscopy<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Topic\/pflanzenzelle<\/a>> # Pflanzenzelle<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Pflanzenzelle<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Topic\/plant_physiology<\/a>> # plant physiology<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"plant physiology<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Topic\/plantenfysiologie<\/a>> # plantenfysiologie<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"plantenfysiologie<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Topic\/plantkunde<\/a>> # plantkunde<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"plantkunde<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Topic\/plants<\/a>> # Plants<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Plants<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Topic\/resonance_magnetique_nucleaire<\/a>> # R\u00E9sonance magn\u00E9tique nucl\u00E9aire<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"R\u00E9sonance magn\u00E9tique nucl\u00E9aire<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Topic\/spectroscopie_de_la_resonance_magnetique_nucleaire<\/a>> # Spectroscopie de la r\u00E9sonance magn\u00E9tique nucl\u00E9aire<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Spectroscopie de la r\u00E9sonance magn\u00E9tique nucl\u00E9aire<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Topic\/tracer_technieken<\/a>> # tracer technieken<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"tracer technieken<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Topic\/tracer_techniques<\/a>> # tracer techniques<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"tracer techniques<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Topic\/tracers<\/a>> # tracers<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"tracers<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/id.loc.gov\/authorities\/subjects\/sh85102843<\/a>> # Plants--Analysis<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Plants--Analysis<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/id.loc.gov\/vocabulary\/countries\/gw<\/a>>\u00A0\u00A0\u00A0\u00A0a \nschema:Place<\/a> ;\u00A0\u00A0\u00A0\ndcterms:identifier<\/a> \"gw<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/id.worldcat.org\/fast\/1040325<\/a>> # Nuclear magnetic resonance<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Nuclear magnetic resonance<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/id.worldcat.org\/fast\/1065831<\/a>> # Plants--Analysis<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Intangible<\/a> ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Plants--Analysis<\/span>\"@en<\/a> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/viaf.org\/viaf\/108295624<\/a>> # John F. Jackson<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Person<\/a> ;\u00A0\u00A0\u00A0\nschema:birthDate<\/a> \"1935<\/span>\" ;\u00A0\u00A0\u00A0\nschema:familyName<\/a> \"Jackson<\/span>\" ;\u00A0\u00A0\u00A0\nschema:givenName<\/a> \"John F.<\/span>\" ;\u00A0\u00A0\u00A0\nschema:givenName<\/a> \"J. F.<\/span>\" ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"John F. Jackson<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/viaf.org\/viaf\/162089832<\/a>> # C. Abell<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Person<\/a> ;\u00A0\u00A0\u00A0\nschema:familyName<\/a> \"Abell<\/span>\" ;\u00A0\u00A0\u00A0\nschema:givenName<\/a> \"C.<\/span>\" ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"C. Abell<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/viaf.org\/viaf\/66526639<\/a>> # Hans F. Linskens<\/span>\n\u00A0\u00A0\u00A0\u00A0a \nschema:Person<\/a> ;\u00A0\u00A0\u00A0\nschema:birthDate<\/a> \"1921<\/span>\" ;\u00A0\u00A0\u00A0\nschema:familyName<\/a> \"Linskens<\/span>\" ;\u00A0\u00A0\u00A0\nschema:givenName<\/a> \"Hans F.<\/span>\" ;\u00A0\u00A0\u00A0\nschema:givenName<\/a> \"H. F.<\/span>\" ;\u00A0\u00A0\u00A0\nschema:name<\/a> \"Hans F. Linskens<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/worldcat.org\/isbn\/9780387159102<\/a>>\u00A0\u00A0\u00A0\u00A0a \nschema:ProductModel<\/a> ;\u00A0\u00A0\u00A0\nschema:isbn<\/a> \"038715910X<\/span>\" ;\u00A0\u00A0\u00A0\nschema:isbn<\/a> \"9780387159102<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/worldcat.org\/isbn\/9783540159100<\/a>>\u00A0\u00A0\u00A0\u00A0a \nschema:ProductModel<\/a> ;\u00A0\u00A0\u00A0\nschema:isbn<\/a> \"354015910X<\/span>\" ;\u00A0\u00A0\u00A0\nschema:isbn<\/a> \"9783540159100<\/span>\" ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/www.worldcat.org\/oclc\/610543767<\/a>>\u00A0\u00A0\u00A0\u00A0a \nschema:CreativeWork<\/a> ;\u00A0\u00A0\u00A0\nrdfs:label<\/a> \"Nuclear magnetic resonance.<\/span>\" ;\u00A0\u00A0\u00A0\nschema:description<\/a> \"Online version:<\/span>\" ;\u00A0\u00A0\u00A0\nschema:isSimilarTo<\/a> <http:\/\/www.worldcat.org\/oclc\/13095988<\/a>> ; # Nuclear magnetic resonance<\/span>\n\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/www.worldcat.org\/title\/-\/oclc\/13095988<\/a>>\u00A0\u00A0\u00A0\u00A0a \ngenont:InformationResource<\/a>, genont:ContentTypeGenericResource<\/a> ;\u00A0\u00A0\u00A0\nschema:about<\/a> <http:\/\/www.worldcat.org\/oclc\/13095988<\/a>> ; # Nuclear magnetic resonance<\/span>\n\u00A0\u00A0\u00A0\nschema:dateModified<\/a> \"2019-06-20<\/span>\" ;\u00A0\u00A0\u00A0\nvoid:inDataset<\/a> <http:\/\/purl.oclc.org\/dataset\/WorldCat<\/a>> ;\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n
<http:\/\/www.worldcat.org\/title\/-\/oclc\/13095988#PublicationEvent\/berlin_new_york_springer_verlag_1986<\/a>>\u00A0\u00A0\u00A0\u00A0a \nschema:PublicationEvent<\/a> ;\u00A0\u00A0\u00A0\nschema:location<\/a> <http:\/\/dbpedia.org\/resource\/New_York_City<\/a>> ; # New York<\/span>\n\u00A0\u00A0\u00A0\nschema:location<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Place\/berlin<\/a>> ; # Berlin<\/span>\n\u00A0\u00A0\u00A0\nschema:organizer<\/a> <http:\/\/experiment.worldcat.org\/entity\/work\/data\/355575937#Agent\/springer_verlag<\/a>> ; # Springer-Verlag<\/span>\n\u00A0\u00A0\u00A0\u00A0.\n\n\n<\/div>\n\n

Content-negotiable representations<\/p>\n