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Photorespiration : methods and protocols

Author: Alisdair R Fernie; Hermann Bauwe; Andreas P M Weber
Publisher: New York, NY : Humana Press, 2017.
Series: Methods in molecular biology (Clifton, N.J.)
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This volume presents a comprehensive overview of contemporary methods to analyze photorespiration in higher plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Read more...
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Details

Genre/Form: Laboratory Manual
Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Photorespiration.
New York, NY : Humana Press, 2017
(OCoLC)987282604
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Alisdair R Fernie; Hermann Bauwe; Andreas P M Weber
ISBN: 9781493972258 1493972251
OCLC Number: 1001807682
Notes: Includes index.
Description: 1 online resource (xiv, 274 pages) : illustrations (some color)
Contents: Estimation of Photorespiratory Fluxes by Gas Exchange --
Measurement of Transcripts Associated with Photorespiration and Related Redox Signaling --
Measurement of Enzyme Activities --
In vitro Alkylation Methods for Assessing the Protein Redox State --
Dimethyl-labeling Based Quantification of the Lysine Acetylome and Proteome of Plants --
In vitro Analysis of Metabolite Transport Proteins --
Quantification of Photorespiratory Intermediates by Mass Spectrometry-based Approaches --
Targeted Isolation and Characterization of T-DNA Mutants Defective in Photorespiration --
Exploiting Natural Variation to Discover Candidate Genes Involved in Photosynthesis-Related Traits --
Metabolic Engineering of Photorespiration --
13CO2 Labelling and Mass Spectral Analysis of Photorespiration --
Isotopically Nonstationary Metabolic Flux Analysis (INST-MFA) of Photosynthesis and Photorespiration in Plants --
Genome-scale Modeling of Photorespiratory Pathway Manipulation --
Kinetic Modelling of Photorespiration --
Investigating the Role of the Photorespiratory Pathway in Non-photosynthetic Tissues --
Studying the Function of the Phosphorylated Pathway of Serine Biosynthesis in Arabidopsis thaliana --
Light Microscopy, Transmission Electron Microscopy and Immunohistochemistry Protocols for Studying Photorespiration.
Series Title: Methods in molecular biology (Clifton, N.J.)
Responsibility: edited by Alisdair R. Fernie, Hermann Bauwe, Andreas P.M. Weber.

Abstract:

This volume presents a comprehensive overview of contemporary methods to analyze photorespiration in higher plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Photorespiration: Methods and Protocols aims to ensure successful results in the further study of this vital field.

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