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Plant vacuolar trafficking : methods and protocols

Author: Cláudia Pereira
Publisher: New York, NY : Humana Press, 2018.
Series: Methods in molecular biology (Clifton, N.J.), v. 1789.
Edition/Format:   eBook : Document : EnglishView all editions and formats

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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manual
Additional Physical Format: (OCoLC)1023525313
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Cláudia Pereira
ISBN: 9781493978564 149397856X 1493978551 9781493978557
OCLC Number: 1041852870
Notes: Includes index.
Description: 1 online resource (xii, 222 pages) : illustrations (some color).
Contents: Green fluorescent seed, to evaluate vacuolar trafficking in Arabidopsis seeds / Tomoo Shimada, Kentaro Fuji, Takuji Ichino, Ooi-Kock Teh, Yasuko Koumoto, Ikuko Hara-Nishimura --
Detection of CRISPR/Cas9-induced genomic fragment deletions in barley and generation of homozygous edited lines via embryogenic pollen culture / Eszter Kapusi, Eva Stöger --
Vacuolar sorting determinants: isolation and study / Bruno Peixoto, Susana Pereira, Cláudia Pereira, José Pissarra --
Vacuole-targeted proteins: ins and outs of subcellular localization studies / Inês Carqueijeiro, Liuda J. Sepúlveda, Angela Mosquera, Richard Payne, Cyrielle Corbin, Nicolas Papon, Thomas Dugé de Bernonville, Sébastien Besseau, Arnaud Lanoue, Gaëlle Glévarec, Marc Clastre, Benoit St-Pierre, Lucia Atehortùa, Nathalie Giglioli-Guivarc'h, Sarah E. O'Connor, Audrey Oudin, Vincent Courdavault --
Plant cell vacuoles: staining and fluorescent probes / Giovanni Stefano, Luciana Renna, Federica Brandizzi --
Vacuolar targeting and characterization of recombinant antibodies / Carolina Gabriela Ocampo, Silvana Petruccelli --
Isolation of vacuoles from the leaves of the medicinal plant Catharanthus roseus / Inês Carqueijeiro, Henrique Noronha, Sara Bettencourt, Joana G. Guedes, Patrícia Duarte, Hernâni Gerós, Marinana Sottomayor --
Flow cytometry and fluorescence microscopy as tools for structural and functional analysis of vacuoles isolated from yeast and plant cells / Jorge M.P. Rodrigues, Cátia S. Pereira, Natacha Fontes, Hernâni Gerós, Manuela Côrtes-Real --
Live cell imaging and confocal microscopy / Luciana Renna, Giovanni Stefano, Federica Brandizzi --
Imaging vacuolar anthocyanins with fluorescence lifetime microscopy (FLIM) / Alexandra Chanoca, Brian Burkel, Erich Grotewold, Kevin W. Eliceiri, Marisa S. Otegui --
The use of drugs in the study of vacuole morphology and trafficking to the vacuole in Arabidopsis thaliana / Ricardo Tejos, Claudio Osorio-Navarro, Lorena Norambuena --
Use of brefeldin A and wormannin to dissect post-golgi organelles related to vacuolar transport in Arabidopsis thaliana / Junpei Takagi, Tomohiro Uemura --
Use Endosidin2 to study plant exocytosis and vacuolar trafficking / Lei Huang, Chunhua Zhang --
Visualization of RMRs (Receptor membrane RING-H2) dimerization in Nicotiana benthamiana leaves using a bimolecular fluorescence complementation (BiFC) assay / Alessandro Occhialini --
Vacuole dynamics in rice cells invaded by the blast fungus / Kiersun Jones, Chang Hyun Khang --
Characterization of plant glycoproteins: analysis of plant glycopeptide mass spectrometry data with plantGlycoMS, a package in the R statistical computing environment / Margaret R. Baker, Travers Ching, David L. Tabb, Qing X. Li.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1789.
Responsibility: edited by Cláudia Pereira.



This volume provides a collection of state-of-the-art protocols on plant vacuolar trafficking. Chapters guide the reader through design and preparation of fusion proteins, expression vectors, and detailed analysis of different microscopy techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Plant Vacuolar Trafficking: Methods and Protocols aims to be a highly valuable resource for researchers interested in learning more about this field.


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