skip to content
Primary glioblastoma cultures: can profiling of stem cell markers predict radiotherapy sensitivity?. Preview this item
ClosePreview this item
Checking...

Primary glioblastoma cultures: can profiling of stem cell markers predict radiotherapy sensitivity?.

Author: Dieter LemkeMarkus WeilerJonas BlaesBenedikt WiestlerLeonie JestaedtAll authors
Publisher: Oxford : Blackwell, 2014.
Edition/Format:   Downloadable article : Document   Computer File : English
Publication:Journal of neurochemistry
Summary:
Abstract Human glioblastomas may be hierarchically organized. Within this hierarchy, glioblastoma-initiating cells have been proposed to be more resistant to radiochemotherapy and responsible for recurrence. Here, established stem cell markers and stem cell attributed characteristics such as self-renewal capacity and tumorigenicity have been profiled in primary glioblastoma cultures to predict radiosensitivity.  Read more...
Rating:

(not yet rated) 0 with reviews - Be the first.

Subjects
More like this

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Material Type: Document
Document Type: Article, Computer File
All Authors / Contributors: Dieter Lemke; Markus Weiler; Jonas Blaes; Benedikt Wiestler; Leonie Jestaedt; Ann-Catherine Klein; Sarah Löw; Günter Eisele; Bernhard Radlwimmer; David Capper; Kirsten Schmieder; Michel Mittelbronn; Stephanie E Combs; Martin Bendszus; Michael Weller; Michael Platten; Wolfgang Wick
ISSN:0022-3042
OCLC Number: 1021130802
Notes: In: Journal of neurochemistry, Vol. 131, no. 2 (Oct. 2014), p.251-264.
Description: 1 online resource

Abstract:

Abstract Human glioblastomas may be hierarchically organized. Within this hierarchy, glioblastoma-initiating cells have been proposed to be more resistant to radiochemotherapy and responsible for recurrence. Here, established stem cell markers and stem cell attributed characteristics such as self-renewal capacity and tumorigenicity have been profiled in primary glioblastoma cultures to predict radiosensitivity. Furthermore, the sensitivity to radiotherapy of different subpopulations within a single primary glioblastoma culture was analyzed by a flow cytometric approach using Nestin, SRY (sex-determining region Y)-box 2 (SOX2) and glial fibrillary acidic protein. The protein expression of Nestin and SOX2 as well as the mRNA levels of Musashi1, L1 cell adhesion molecule, CD133, Nestin, and pleiomorphic adenoma gene-like 2 inversely correlated with radioresistance in regard to the clonogenic potential. Only CD44 protein expression correlated positively with radioresistance. In terms of proliferation, Nestin protein expression and Musashi1, pleiomorphic adenoma gene-like 2, and CD133 mRNA levels are inversely correlated with radioresistance. Higher expression of stem cell markers does not correlate with resistance to radiochemotherapy in the cancer genome atlas glioblastoma collective. SOX2 expressing subpopulations exist within single primary glioblastoma cultures. These subpopulations predominantly form the proliferative pool of the primary cultures and are sensitive to irradiation. Thus, profiling of established stem cell markers revealed a surprising result. Except CD44, the tested stem cell markers showed an inverse correlation between expression and radioresistance. Markers used to define glioma-initiating cells (GIC) are generally not defining a more resistant, but rather a more sensitive group of glioma cells. An exemption is CD44 expression. Also proliferation of the GIC culture itself was not systematically associated with radiosensitivity or - resistance, but a SOX-2 positive, proliferative subgroup within a GIC culture is showing the highest radiosensitivity.

Reviews

User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.

Similar Items

Related Subjects:(1)

Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.