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Principles of gene manipulation : an introduction to genetic engineering

Author: R W Old; S B Primrose
Publisher: Oxford : Blackwell Scientific, 1994.
Series: Studies in microbiology.
Edition/Format:   Print book : English : 5th edView all editions and formats
Summary:

The basic philosophy of this text is to present the principles and techniques of gene manipulation in sufficient detail to enable the non-specialist reader to understand them. The book has been  Read more...

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Document Type: Book
All Authors / Contributors: R W Old; S B Primrose
ISBN: 0632037121 9780632037124
OCLC Number: 32132450
Notes: Previous edition: 1989.
Description: vi, 474 pages
Contents: Introduction to Gene Manipulation Why do we clone genes? How do we clone genes?- the basic technique Cutting and joining DNA Cloning Genes in E. coli Plasmids as vectors Phage and cosmid vectors Cloning strategies, gene libraries and cDNA cloning Recombinant selection and screening Expression of cloned genes Analyzing DNA nucleotide sequences Changing genes - macro- and micro-modifications The polymerase chain reaction - an all embracing technique Impact of recombinant DNA on human genetics Cloning in Organisms other than E. coli Cloning in bacteria other than E. coli Cloning in yeast and other microbial eukaryotes Gene transfer to plants Introducing genes into animal cells Transferring genes into animal oocytes, eggs and embryos Biotechnology: The generation of novelty
Series Title: Studies in microbiology.
Responsibility: R.W. Old, S.B. Primrose.

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