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Protein lipidation protocols

Author: Michael H Gelb
Publisher: Totowa, N.J. : Humana Press, ©1999.
Series: Methods in molecular biology (Clifton, N.J.), v. 116.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Publication:Springer Protocols
Summary:
In Protein Lipidation Protocols, Michael Gelb brings together a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods-many never published before in a "hands-on" format-deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Included are novel techniques for  Read more...
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Genre/Form: Laboratory Manual
Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Protein lipidation protocols.
Totowa, N.J. : Humana Press, ©1999
(DLC) 98050534
(OCoLC)40311554
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Michael H Gelb
ISBN: 0585238987 9780585238982 1592592643 9781592592647 0896035344 9780896035348
OCLC Number: 45729384
Description: 1 online resource (x, 240 pages) : illustrations
Contents: In Vitro Analysis of GPI Biosynthesis in Mammalian Cells / Victoria L. Stevens --
Selection of Mammalian Cell Mutants in GPI Biosynthesis / Victoria L. Stevens --
Analysis of the Cell-Surface Distribution of GPI-Anchored Proteins / Satyajit Mayor --
Imaging Fluorescence Resonance Energy Transfer as Probe of Membrane Organization and Molecular Associations of GPI-Anchored Proteins / Anne K. Kenworthy and Michael Edidin --
Purification of Caveolae-Derived Membrane Microdomains Containing Lipid-Anchored Signaling Molecules, Such as GPI-Anchored Proteins, H-Ras, Src-Family Tyrosine Linases, eNOS and G-Protein a-, b-, and g-Subunits / Michael P. Lisanti, Massimo Sargiacomo, and Philipp E. Scherer --
Analysis of Lipids in Caveolae / Jun Liu and Jan E. Schnitzer --
Analysis of the Carbohydrate Components of Glycosylphosphatidylinositol Structures Using Fluorescent Labeling / Nicole Zitzmann and Michael A.J. Ferguson --
Analysis of the Lipid Moiety of GPI Anchor in the Yeast Saccharomyces cerevisiae / Fulvio Reggiori, Elisabeth Canivenc-Gansel, and Andreas Conzelmann --
Rapid Identification of Cysteine-Linked Isoprenyl Groups By Metabolic Labeling with [3H]Farnesol and [3H]Geranylgeraniol / Douglas A. Andres, Dean C. Crick, Brian S. Finlin, and Charles J. Waechter --
Incorporation of Radiolabeled Prenyl Alcohols and Their Analogs into Mammalian Cell Proteins: A Useful Tool for Studying Protein Prenylation / Alberto Corsini, Christopher C. Farnsworth, Paul McGeady, Michael H. Gelb, and John A. Glomset --
Reconstitution of Yeast Farnesyltransferase from Individually Purified Subunits / Jun Urano and Fuyuhiko Tamanoi --
Probing the Role of H-Ras Lipidation for Signaling Functions in Xenopus laevis Oocytes / Thomas Dudler and Michael H. Gelb --
Fluorescence Measurement of Lipid-Binding Affinity and Interbilayer Transfer of Bimane-Labeled Lipidated Peptides / John R. Silvius --
Determination of the Kinetics of Intervesicle Transfer and Transbilayer Diffusion of Bimane-Labeled Lipidated Peptides / John R. Silvius --
Preparation and Assay of Myristoyl-CoA: Protein N-Myristoyltransferase / Rajala V.S. Raju and Rajendra K. Sharma --
Metabolic Labeling of Protein-Derived Lipid Thioesters in Palmitoyl-Protein Thioesterase-Deficient Cells / Sandra L. Hofmann and Jui-Yun Lu. Fatty Acid Analysis of Protein-Derived Lipid Thioesters Isolated from Palmitoyl-Protein Thioesterase-Deficient Cells / Sandra L. Hofmann and Linda A. Verkruyse.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 116.
Responsibility: edited by Michael H. Gelb.

Abstract:

This text contains a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods deal with glycosyl  Read more...

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