Protocol: an improved and universal protocol for whole-mount immunolocalization in plants (eBook, 2015) [WorldCat.org]
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Protocol: an improved and universal protocol for whole-mount immunolocalization in plants

Author: Taras PasternakOlaf TietzKatja RappRoland NitschkeKlaus PalmeAll authors
Publisher: Freiburg Universität 2015
Edition/Format:   eBook : Document : English
Summary:
Abstract: Rapid advances in microscopy have boosted research on cell biology. However sample preparation enabling excellent reproducible tissue preservation and cell labeling for in depth microscopic analysis of inner cell layers, tissues and organs still represents a major challenge for immunolocalization studies. Here we describe a protocol for whole-mount immunolocalization of proteins which is applicable to a  Read more...
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Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Taras Pasternak; Olaf Tietz; Katja Rapp; Roland Nitschke; Klaus Palme; Molekulare Pflanzenphysiologie; Albert-Ludwigs-Universität Freiburg Institut für Biologie II; Albert-Ludwigs-Universität Freiburg Fakultät für Biologie; Albert-Ludwigs-Universität Freiburg Centre for Biological Signalling Studies; Zentrum für Biosystemanalyse; Albert-Ludwigs-Universität Freiburg Freiburg Institute of Advanced Studies; Albert-Ludwigs-Universität Freiburg
OCLC Number: 1003248418
Notes: Plant Methods. 11 (2015), 50, DOI 10.1186/s13007-015-0094-2, issn: 1746-4811
IN COPYRIGHT http://rightsstatements.org/page/InC/1.0 rs
Description: Online-Ressource

Abstract:

Abstract: Rapid advances in microscopy have boosted research on cell biology. However sample preparation enabling excellent reproducible tissue preservation and cell labeling for in depth microscopic analysis of inner cell layers, tissues and organs still represents a major challenge for immunolocalization studies. Here we describe a protocol for whole-mount immunolocalization of proteins which is applicable to a wide range of plant species. The protocol is improved and robust for optimal sample fixation, tissue clearing and multi-protein staining procedures and can be used in combination with simultaneous detection of specific sequences of nucleic acids. In addition, cell wall and nucleus labelling can be implemented in the protocol, thereby allowing a detailed analysis of morphology and gene expression patterns with single-cell resolution. Besides enabling accurate, high resolution and reproducible protein detection in expression and localization studies, the procedure takes a single working day to complete without the need for robotic equipment.<br><br>Keywords<br><br>Immunolocalization - Tissue multi-protein expression - Whole-mount - 3D reconstruction - Protein-protein interaction

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