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Protocols for oligonucleotide conjugates : synthesis and analytical techniques

Author: Sudhir Agrawal
Publisher: Totowa, N.J. : Humana Press, ©1994.
Series: Methods in molecular biology (Clifton, N.J.), v. 26.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
You will easily synthesize and analyze oligonucleotide conjugates by following the step-by-step protocols presented in this volume. These techniques are widely used by all molecular biologists and antisense researchers and find special application by pharmacologists working in new drug development and quality assurance assay.
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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Protocols for oligonucleotide conjugates
(DLC) 93023127
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Sudhir Agrawal
ISBN: 0896032523 9780896032521 9781592595136 1592595138
OCLC Number: 55638682
Language Note: English.
Notes: Source of title; title from title screen.
Description: 1 online resource (xiv, 377 pages) : illustrations.
Contents: Protecting groups in oligonucleotide synthesis / Etienne Sonveaux --
Incorporation of modified bases into oligonucleotides / Rich B. Meyer --
Functionalization of oligonucleotides with amino groups and attachment of amino specific reporter groups / Sudhir Agrawal --
Functionalization of oligonucleotides by the incorporation of thio-specific reporter groups / Jacqueline A Fidanza, Hiroaki Ozaki, Larry W. McLaughlin --
Postsynthesis functionalization of oligonucleotides / Barbara C. Chu, Leslie E. Orgel --
Oligonucleotide-enzyme conjugates / Jerry L Ruth --
Oligonucleotides containing degenerate bases: synthesis and uses / Paul Kong Lin, Daniel M. Brown --
Synthesis of ¹⁵N-labeled DNA fragments / Roger A Jones --
Analysis and purification of synthetic oligonucleotides by high-performance liquid chromatography / William J. Warren, George Vella --
Sequence analysis of oligodeoxyribonucleotides / Diane M. Black, Peter T. Gilham --
Gel-capillary electrophoresis analysis of oligonucleotides / Alex Andrus --
Nuclear magnetic resonance studies of oligonucleotides / Jerzy W. Jaroszewski, Siddhartha Roy, Jack S. Cohen --
Mass spectrometry of nucleotides and oligonucleotides / Thomas D. McClure, Karl H. Schram --
Extracting thermodynamic data from equilibrium melting curves for oligonucleotide order-disorder transitions / Kenneth J. Breslauer.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 26.
Responsibility: edited by Sudhir Agrawal ; foreword by H. Gobind Khorana.
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Abstract:

In this book, leading researchers provide the latest protocols for synthesizing oligonucleotide conjugates, and explain the latest techniques for analyzing them. These techniques are widely used by  Read more...

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...an extremely useful and well-produced volume...a very well-qualified and prestigious group of authors...produce an up-to-date review of current topics in this important and growing field. - Trends Read more...

 
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